Effects of Tocotrienols on Life Span and Protein Carbonylation in Caenorhabditis elegans

Adachi, Hiroshi; Ishii, Naoaki

doi:10.1093/gerona/55.6.b280

Cited by 127 publications

(66 citation statements)

References 40 publications

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“…Lifespan studies were performed as in Adachi and Ishii (2000). Eggs were collected from gravid nematodes by standard hypochlorite treatment and grown on standard nematode growth media (NGM) plates at 16°C to prevent dauer formation in daf-2 worms until L4-adult stage.…”

Section: Lifespan Assaysmentioning

confidence: 99%

Evidence for only two independent pathways for decreasing senescence in Caenorhabditis elegans

Yen

Mobbs

2009

AGE

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Cold temperature, dietary restriction, reduced insulin/insulin-like growth factor signaling, and mutations in mitochondrial genes have all been shown to extend the lifespan of Caenorhabditis elegans (Kenyon et al., Nature 366:461-464, 1993; Klass, Mech Ageing Dev 6:413-429, 1977; Lakowski and Hekimi, Science 272:1010-1013. Additionally, all of them extend the lifespan of mice (Bluher et al., Science 299:572-574, 2003; Conti et al., Science 314:825-828, 2006; Holzenberger et al., Nature 421:182-187, 2003; Liu et al., Genes Dev 19:2424-2434 Weindruch and Walford, Science 215:1415-1418, 1982. The mechanism by which these treatments extend lifespan is currently unknown, but our study uses an epistatic approach to show that these four manipulations are mainly additive in terms of lifespan. Classical interpretation of this data suggests that these manipulations are independent of each other. However, using a Gompertz mortality rate analysis, the maximum mortality rate doubling time can be achieved through the use of only dietary restriction and cold temperature, suggesting that the mechanisms by which cold temperature and caloric restriction extend lifespan are the only independent mechanisms.

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Section: Lifespan Assaysmentioning

confidence: 99%

Evidence for only two independent pathways for decreasing senescence in Caenorhabditis elegans

Yen

Mobbs

2009

AGE

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“…Other notable accounts involving antioxidants and their effects on invertebrate lifespan include studies on Vitamin E (Harrington and Harley 1988;Adachi and Ishii 2000;Ishii et al 2004;Driver and Georgeou 2003), Coenzyme Q 10 (Ishii et al 2004), and elegans. Synchronous populations of hermaphrodites were cultured in liquid medium as previously described and transferred to fresh media each day of the lifespan experiment.…”

Section: Pharmacological Interventions That Increase Lifespanmentioning

confidence: 99%

“…However, with the exception of Coenzyme Q 10 , the other antioxidants mentioned have been shown to have differing lifespan effects both in the same organism (Harrington and Harley 1988;Adachi and Ishii 2000;Ishii et al 2004;Driver and Georgeou 2003) and between species (Driver and Georgeou 2003;Bonilla et al 2002;Izmaylov and Obukhova 1999;Anisimov et al 1997;Anisimov 2003).…”

Section: Pharmacological Interventions That Increase Lifespanmentioning

confidence: 99%

Pharmacological intervention in invertebrate aging

Lithgow

Gill

Olsen

et al. 2005

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Prompted by successful genetic interventions in aging we now consider the utility of pharmacological interventions. A few clear cases of lifespan extension resulting from exposure to compounds suggest that aging can be slowed and that the pharmacology of lifespan extension is a feasible area for research. Compounds that slow aging may prompt the rational design of therapeutics against age-related diseases. Here we evaluate invertebrate models for drug discovery in aging and outline how the field may develop from these simple first steps.

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“…Two previous studies had assessed the effect of tocopherols on C. elegans lifespan and found that α-tocopherol either had no effect (Adachi and Ishii, 2000) or a slightly beneficial effect (Harrington and Harley, 1988). In our study, we compared the effect of α-or γ-tocopherols on C. elegans lifespan at either 20 or 200 μg/mL.…”

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confidence: 94%

Comparative approaches to facilitate the discovery of prolongevity interventions: Effects of tocopherols on lifespan of three invertebrate species

Zou

Sinclair

Wilson

et al. 2007

Mechanisms of Ageing and Development

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SummaryMany compounds hold promise for pharmacologic manipulation of aging. However, such claims are difficult to investigate due to time and budget constraints. Here, we took a comparative approach, using short-lived invertebrate species, to directly test the effects of two tocopherols (vitamin E) on longevity. γ-tocopherol represents the most abundant tocopherol in the Western diet, while α-tocopherol is selectively enriched in human plasma. Both isoforms demonstrate antioxidant activity and are proposed to have anti-aging activities. We compared the effects of α-and γ-tocopherol supplementation on lifespan in three invertebrate species. γ-, but not α-, tocopherol slightly extended lifespan in nematodes, but neither significantly affected lifespan in two fly species. This study shows that a comparative approach, utilizing multiple invertebrate species, can increase the robustness of invertebrate-based pilot screens for prolongevity interventions.Pharmacological and nutritional approaches to retard aging and increase longevity have been proposed (Floyd, 2006, Joseph et al., 2005. Large-scale screens are being considered for a number of model organisms to accelerate discovery of compounds with the greatest potential for extending lifespan (Bauer et al., 2004, Wang et al., 2004. One class of compounds with potentially beneficial effects on aging are the tocopherol isomers found in Vitamin E and which are found naturally in plant oils (Ames et al., 2005). Vitamin E is a mixture of several related tocopherol and tocotrienol isomers, including the α-and γ-tocopherols. The Western diet is enriched in γ-tocopherol, but α-tocopherol is selectively retained in human plasma (Jiang et al., 2001, Tucker andTownsend, 2005). Both tocopherols are derived from fatty acids and exist within membranes, where they affect membrane fluidity and can act as antioxidants.The high antioxidant capacity of vitamin E makes it a candidate for a prolongevity therapy, since aging is correlated with increased oxidative stress in cells (Ames et al., 1993, Harman, 1 These authors contributed equally to this work. * Corresponding author: CAW: Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, 5600 Nathan Shock Drive, Baltimore MD 21224; Tel: 410-558-8566; Fax: 410-558-8323; wolkowca@grc.nia.nih.gov.Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. One efficient strategy for directly examining whether these compounds have any effect on lifespan is to determine whether supplementation can affect lifespan in short-lived invertebrate species, which offer models for aging in...

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Effects of Tocotrienols on Life Span and Protein Carbonylation in Caenorhabditis elegans

Cited by 127 publications

References 40 publications

Evidence for only two independent pathways for decreasing senescence in Caenorhabditis elegans

Evidence for only two independent pathways for decreasing senescence in Caenorhabditis elegans

Pharmacological intervention in invertebrate aging

Comparative approaches to facilitate the discovery of prolongevity interventions: Effects of tocopherols on lifespan of three invertebrate species

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