Effects of Tityus serrulatus scorpion venom on lung mechanics and inflammation in mice

Peres, Ana Claudia Paneque; Nonaka, Paula Naomi; Carvalho, Paulo de Tarso Camillo de; Toyama, Marcos H.; Silva, César Augusto Melo e; Vieira, Rodolfo de Paula; Dolhnikoff, Marisa; Zamunér, Stella Regina; Oliveira, Luís Vicente Franco de

doi:10.1016/j.toxicon.2009.02.002

Cited by 26 publications

(16 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a subsequent step, effective lung resistance (R L ) and elastance (E L ) were computed by linear regression fitting of the recorded signals P tr , V′, and V to the conventional respiratory mechanics model P tr = P o + E L × V + R L × V′, where P o is a parameter to account for the external positive end-expiratory pressure applied by the ventilator. For each animal, R L and E L were computed from data obtained during 5 breathing cycles [21, 22]. …”

Section: Methodsmentioning

confidence: 99%

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

et al. 2018

View full text Add to dashboard Cite

Background: Pseudomonas aeruginosa (PS) infection results in severe morbidity and mortality, especially in immune-deficient populations. Aerobic exercise (AE) modulates the immune system, but its effects on the outcomes of pulmonary PS infection in elderly mice are unknown. Methods: BALB/c mice (24 weeks old) were randomized to sedentary, exercise (EX), PS, and PS + EX groups for the acute experimental setting, and PS and PS + EX groups for the chronic setting. Low-intensity AE was performed for 5 weeks, 60 min/day; 24 h after the final AE session, mice were inoculated with 5 × 10⁴ colony-forming units (CFU) of PS, and 24 h and 14 days after PS inoculation, mice were studied. Results: AE inhibited PS colonization (p < 0.001) and lung inflammation (total cells, neutrophils, lymphocytes [p < 0.01] in bronchoalveolar lavage [BAL]), with significant differences in BAL levels of IL-1β (p < 0.001), IL-6 (p < 0.01), CXCL1 (p < 0.001), and TNF-α (p < 0.001), as well as parenchymal neutrophils (p < 0.001). AE increased BAL levels of IL-10 and parenchymal (p < 0.001) and epithelial (p < 0.001) IL-10 expression, while epithelial (p < 0.001) and parenchymal (p < 0.001) NF-κB expression was decreased. AE diminished pulmonary lipid peroxidation (p < 0.001) and increased glutathione peroxidase (p < 0.01). Pre-incubation of BEAS-2B with IL-10 inhibited PS-induced epithelial cell expression of TNF-α (p < 0.05), CD40 (p < 0.01), and dichlorodihydrofluorescein diacetate (p < 0.05). Conclusions: AE inhibits PS-induced lung inflammation and bacterial colonization in elderly mice, involving IL-10/NF-κB, and redox signaling.

show abstract

Section: Methodsmentioning

confidence: 99%

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Paneque Peres et al 2009 [135] shown that the Brazilian scorpion T. serrulatus resulted in increased lung, kidney, liver, and heart inflammation, characterized by an increased density of mononuclear cells after injection in rats. These authors concluded that this venom leads to acute lung injury, characterized by altered lung mechanisms and increased pulmonary inflammation.…”

Section: Organ Dysfunction and The Inflammatory Responsementioning

confidence: 99%

Scorpion Venom and the Inflammatory Response

Petricevich

2010

Mediators of Inflammation

200

135

View full text Add to dashboard Cite

Scorpion venoms consist of a complex of several toxins that exhibit a wide range of biological properties and actions, as well as chemical compositions, toxicity, and pharmacokinetic and pharmacodynamic characteristics. These venoms are associated with high morbility and mortality, especially among children. Victims of envenoming by a scorpion suffer a variety of pathologies, involving mainly both sympathetic and parasympathetic stimulation as well as central manifestations such as irritability, hyperthermia, vomiting, profuse salivation, tremor, and convulsion. The clinical signs and symptoms observed in humans and experimental animals are related with an excessive systemic host inflammatory response to stings and stings, respectively. Although the pathophysiology of envenomation is complex and not yet fully understood, venom and immune responses are known to trigger the release of inflammatory mediators that are largely mediated by cytokines. In models of severe systemic inflammation produced by injection of high doses of venom or venoms products, the increase in production of proinflammatory cytokines significantly contributes to immunological imbalance, multiple organ dysfunction and death. The cytokines initiate a cascade of events that lead to illness behaviors such as fever, anorexia, and also physiological events in the host such as activation of vasodilatation, hypotension, and increased of vessel permeability.

show abstract

“…Transcriptome analysis showed that sodium channel toxins represent 16% of the transcripts in Ts venom [17]. These neurotoxins disrupt the neuromuscular, cardiovascular, and respiratory systems and elicit a complex pattern of clinical signs and symptoms that can lead to death [9], [24]–[27]. Other important neurotoxins, which represent 22% of Ts venom transcripts [17], act on potassium channels [28]–[30].…”

Section: Introductionmentioning

confidence: 99%

Molecular, Immunological, and Biological Characterization of Tityus serrulatus Venom Hyaluronidase: New Insights into Its Role in Envenomation

et al. 2014

View full text Add to dashboard Cite

BackgroundScorpionism is a public health problem in Brazil, and Tityus serrulatus (Ts) is primarily responsible for severe accidents. The main toxic components of Ts venom are low-molecular-weight neurotoxins; however, the venom also contains poorly characterized high-molecular-weight enzymes. Hyaluronidase is one such enzyme that has been poorly characterized.Methods and principal findingsWe examined clones from a cDNA library of the Ts venom gland and described two novel isoforms of hyaluronidase, TsHyal-1 and TsHyal-2. The isoforms are 83% identical, and alignment of their predicted amino acid sequences with other hyaluronidases showed conserved residues between evolutionarily distant organisms. We performed gel filtration followed by reversed-phase chromatography to purify native hyaluronidase from Ts venom. Purified native Ts hyaluronidase was used to produce anti-hyaluronidase serum in rabbits. As little as 0.94 µl of anti-hyaluronidase serum neutralized 1 LD50 (13.2 µg) of Ts venom hyaluronidase activity in vitro. In vivo neutralization assays showed that 121.6 µl of anti-hyaluronidase serum inhibited mouse death 100%, whereas 60.8 µl and 15.2 µl of serum delayed mouse death. Inhibition of death was also achieved by using the hyaluronidase pharmacological inhibitor aristolochic acid. Addition of native Ts hyaluronidase (0.418 µg) to pre-neutralized Ts venom (13.2 µg venom+0.94 µl anti-hyaluronidase serum) reversed mouse survival. We used the SPOT method to map TsHyal-1 and TsHyal-2 epitopes. More peptides were recognized by anti-hyaluronidase serum in TsHyal-1 than in TsHyal-2. Epitopes common to both isoforms included active site residues.ConclusionsHyaluronidase inhibition and immunoneutralization reduced the toxic effects of Ts venom. Our results have implications in scorpionism therapy and challenge the notion that only neurotoxins are important to the envenoming process.

show abstract

Effects of Tityus serrulatus scorpion venom on lung mechanics and inflammation in mice

Cited by 26 publications

References 36 publications

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

Scorpion Venom and the Inflammatory Response

Molecular, Immunological, and Biological Characterization of Tityus serrulatus Venom Hyaluronidase: New Insights into Its Role in Envenomation

Contact Info

Product

Resources

About