Effects of tidal emersion and marine heatwaves on cuttlefish early ontogeny

Coelho, J.P.; Court, Mélanie; Otjacques, Eve; Lopes, Vanessa M.; Paula, José; Repolho, Tiago; Diniz, Mário; Rosa, Rui

doi:10.1007/s00227-022-04150-8

Cited by 7 publications

(9 citation statements)

References 53 publications

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“…The amount of protein in the samples was quantified following the Bradford method, as described in Coelho et al [31].…”

Section: Total Protein Determinationmentioning

confidence: 99%

Effects of Exposure to Urban Atmospheric Particulate Matter Suspended in Seawater on the Mussel Mytilus galloprovincialis

Rodrigues,

Ferreira,

Duarte

et al. 2024

Environments

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Atmospheric particulate matter (PM) contains numerous constituents, including organic molecules, inorganic ions, and metals, with some of them possessing hazardous properties. Although mainly associated with air pollution, PM can rapidly be transferred from air and land to aquatic ecosystems, and consequently poses a risk to marine biota. The aim of this work was to evaluate how urban atmospheric PM (a standard reference mixture of urban PM, known to contain various organic and inorganic contaminants), suspended in seawater, may cause toxicity in marine organisms. To this purpose, mussels (Mytilus galloprovincialis) were exposed to two concentrations of suspended PM: 5.7 and 11.4 mg/L. After 7, 14, and 21 days, the animals were collected and the gills and digestive gland were analysed for stress biomarkers (CAT, SOD, GPX, GST, MDA, and Ubi). In general, the results show that exposure to different concentrations of PM caused an increase in GST, UBI, and GPx activities compared to their respective controls. The average activities of GST (87.65 ± 30.23 nmol/min/mg of total protein) in the gills of the animals exposed to 11.4 mg/L of PM increased after 21 days of exposure, and the activity of GPx (8.04 ± 3.09 nmol/min/mg of total protein) in the gills increased after 14 days in the animals exposed to 5.7 mg/L of PM. MDA results also provided information on cellular damage, with the most pronounced effects being found in the gills of exposed mussels. This study confirms that mussels are useful as “early warning” indicators of environmental contamination and provides important information on the effects of PM on marine biota.

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“…The amount of protein in the samples was quantified following the Bradford method, as described in Coelho et al [31].…”

Section: Total Protein Determinationmentioning

confidence: 99%

Effects of Exposure to Urban Atmospheric Particulate Matter Suspended in Seawater on the Mussel Mytilus galloprovincialis

Rodrigues,

Ferreira,

Duarte

et al. 2024

Environments

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“…However, the life history of females may also be important for embryo development. Before spawning, female octopuses acquire the energy for egg production directly from food, which is vital for embryos and offspring because their nutritional condition in uence the morphological and biochemical characteristics of the next generation [12][13][14] . Recent studies in O. maya demonstrated that thermally stressed females produce embryos with higher metabolic rates than animals from non-stressed females 15,16 .…”

Section: Introductionmentioning

confidence: 99%

How hard is Octopus maya embryo’s life?

Ramos-Rodriguez,

Ortega,

Mendez-Can

et al. 2024

Preprint

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Embryos inherit ROS (reactive oxygen species) from females, suggesting they are produced in females due to the metabolic processes that occur in the ovary during the last part of the yolk synthesis and implying ROS production in females is a natural part of their reproductive processes. Embryos have a piece of genetic machinery to produce antioxidant/detoxification enzymes. ANTIOX enzymes help reduce ROS levels once organogenesis is completed and indicate the embryos are prepared to buffer and neutralize the ROS inherited from females. Thus, the present study provides some information on SOD (superoxide dismutase), CAT (catalase), and HIF1 (hypoxia-inducible factor 1) gene expressions during embryonic development. CAT gene expression is present from the first developmental stage, increases during organogenesis, and remains stable until hatching indicates that this gene is maintained throughout the embryonic development process. For SOD genes that SOD1 expression is present from the first development stage. Regarding the HIF1A gene, ROS induces its expression and is crucial for embryonic vascularization, suggesting that this gene expression may be elevated during the embryonic development activation stage when mitochondrial ROS production increases. ROS in O. maya eggs were controlled by embryos during the embryo growth phase. When measuring HMR, stimulated by the increase in temperature (30°C), the embryos maintain a metabolic rate very similar to their RMR measured at 24°C.

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“…The eggs are attached to natural or artificial support in the intertidal area, where they are subject at low tide to large variations in temperature, low humidity and high amounts of sunshine (irradiance). The embryonic development of S. officinalisis within the egg involves 30 developmental stages grouped into five different phases: cleavage (stages 1-9), gastrulation (stages 10-13), organogenesis, flat stage (stages 14-18), organogenesis, extension stage (stages [19][20][21][22] and organogenesis, growth stage (stages [23][24][25][26][27][28][29][30] when the embryo has the general adult conformation [9]. The light sensitivity of S. officinalis has been demonstrated in the later stages [growth stage (stages [23][24][25][26][27][28][29][30]] of development [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Enzymatic activities of superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione reductase, glutathione peroxidase (GPx) and catalase (CAT) play an essential role in the detoxifying of ROS into less reactive products [17,18]. Oxidative stress may also activate heat shock proteins (HSPs), especially the HSP70, which play vital roles in protein quality control and in repairing denatured proteins and provide a protective mechanism after exposure to stress [19,20]. Herrera-Vásquez et al [21] and Zhou et al [22] suggest that HSPs and GSTs support ROS processing systems initiated by antioxidant enzymes such as SOD, playing an essential role in the control of ROS levels and oxidative damage in the tolerance response to UVB light and photooxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Does the egg capsule protect against chronic UV-B radiation? A study based on encapsulated and decapsulated embryos of cuttlefish Sepia officinalis

et al. 2023

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Although the egg capsule plays a crucial role in the embryonic development of cephalopods, its ability to protect embryos from Ultraviolet (UV) radiation is unknown. Our study evaluated the photoprotection mechanisms of S. officinalis to UV-B radiation and estimated the ability of the black capsule to act as a physical shield against it. Embryos with and without capsule and juveniles were exposed to four experimental UVB conditions for 55 days. The effects of different UVB doses were evaluated in terms of morphological abnormalities and differences in gene expression between each group. We observed that the development might be severely impaired in embryos exposed to UVB without capsule protection, and these effects were time- and UVB-dose-dependent. In addition, we found variations in gene expression levels (light-sensitive, stress response and DNA repair) in different tissues as a function of UVB doses. We suggest a relationship between morphological abnormalities and the limit of molecular regulation. These results suggest that the quantitative differences in expression are essential for defining the survivability of the embryo face to UVB. Thus, we demonstrated that the egg capsule could ensure successful embryonic development of the cuttlefish S. officinalis even at high doses of UVB.

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Effects of tidal emersion and marine heatwaves on cuttlefish early ontogeny

Cited by 7 publications

References 53 publications

Effects of Exposure to Urban Atmospheric Particulate Matter Suspended in Seawater on the Mussel Mytilus galloprovincialis

Effects of Exposure to Urban Atmospheric Particulate Matter Suspended in Seawater on the Mussel Mytilus galloprovincialis

How hard is Octopus maya embryo’s life?

Does the egg capsule protect against chronic UV-B radiation? A study based on encapsulated and decapsulated embryos of cuttlefish Sepia officinalis

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