Effects of thyroxin (T4) and activin A on in vitro growth of preantral follicles in domestic cats

Wongbandue, Grisnarong; Jewgenow, Katarina; Chatdarong, Kaywalee

doi:10.1016/j.theriogenology.2012.12.008

Cited by 16 publications

(13 citation statements)

References 43 publications

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“…The benefit of the 3D/alginate approach is believed to be related to promotion of cell-to-cell contact and cell-to-extracellular matrix interaction within the follicle, both key to normal folliculogenesis [42]. Thus, we speculate that this same incubation system was at least partially overcoming a subpar microenvironment used in earlier cat investigations [37, 38] that likely was disrupting somatic cell-oocyte communication.…”

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

“…Most studies to-date have relied on a 2-dimensional (2D) culture system and conventional culture drops to grow follicles in vitro [37, 38]. Earlier efforts using that approach have demonstrated that cat preantral follicles (<150 µm in diameter) survive for the first 7 d of culture, but then degenerate [38]. Here, we demonstrated that a 3D culture technique utilizing alginate hydrogel (a system previously developed by others) [11, 39] sustained the ability of cat secondary and early antral follicles to grow and survive in vitro for up to 15 d. Furthermore, unlike in earlier cat studies [37, 38], some of the resident oocytes (up to 21%) recovered from donor follicles cultured in the 3D alginate system retained the capacity to resume meiosis and complete nuclear maturation.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier efforts using that approach have demonstrated that cat preantral follicles (<150 µm in diameter) survive for the first 7 d of culture, but then degenerate [38]. Here, we demonstrated that a 3D culture technique utilizing alginate hydrogel (a system previously developed by others) [11, 39] sustained the ability of cat secondary and early antral follicles to grow and survive in vitro for up to 15 d. Furthermore, unlike in earlier cat studies [37, 38], some of the resident oocytes (up to 21%) recovered from donor follicles cultured in the 3D alginate system retained the capacity to resume meiosis and complete nuclear maturation. In that context, this alternative mode of incubation itself imparted advantages for incubating premature cat follicles, as it has for the mouse [7], human [9, 10, 40] and non-human primate [11, 12].…”

Section: Discussionmentioning

confidence: 99%

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Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model

et al. 2017

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Using the domestic cat as a non-rodent, larger animal model, the objective of the present study was to determine the impact of a brief incubation in a hypertonic microenvironment on (1) ovarian follicle and oocyte growth in vitro, (2) developmental capacity of the resident oocyte, and (3) expression of aquaporin (AQP) genes in parallel with genes involved in the regulation of folliculogenesis. In Study 1: Secondary or early antral follicles encapsulated in alginate were allocated to one of three treatment groups: 1) culture in standard medium at 290 mOsm for 15 d (Control); 2) incubation in 350 mOsm medium for 1 h followed by culture in standard medium for 15 d (Hypertonic-1h); or 3) incubation in 350 mOsm medium for 24 h followed by incubation in standard medium for additional 14 d (Hypertonic-24h). After measuring follicle and oocyte diameters on Day 15, in vitro-grown oocytes were incubated for 24 h before assessing their nuclear status. In Study 2: secondary or early antral follicles were subjected to one of the three treatments: 1) culture in standard medium at 290 mOsm for 48 h; 2) incubation in 350 mOsm medium for 1 h followed by culture in standard medium for additional 47 h; or 3) incubation in 350 mOsm medium for 24 h followed by culture in standard medium for additional 24 h. At the end of the culture period, all follicles were assessed for mRNA level of Cyp17a1, Cyp19a1, Star (Steroidogenesis genes)AQP1, 3, 5, 7 and 8 as well as Fshr using qPCR. Freshly collected follicles also were subjected to gene expression analysis and served as ‘Non-cultured control’. Hypertonic-24h follicles grew larger (P < 0.05) than the control, whereas those in Hypertonic-1h group exhibited intermediate growth, especially when the culture started at the early antral stage. Oocytes in the Hypertonic-24h group were larger and resumed meiosis at a higher rate than in the other treatments. In vitro culture affected (P < 0.05) mRNA expression of Cyp19a1, Star, AQP1 and AQP7 in both secondary and early antral stage while FSHr was only affected in the former compared to the non-cultured control. Pre-incubating follicles in 350 mOsm medium for 24 h enhanced (P < 0.05) Star and AQP7 while decreasing (P < 0.05) AQP1 expression compared to the control in secondary follicles, but not in the early antral stage. In summary, short-term hypertonic exposure promoted cat follicle development in vitro (including the meiotic competence of the enclosed oocyte) possibly through a mechanism that does not involve water transport genes.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model

et al. 2017

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“…The use of T3 and/or T4 in association to other hormones enhance survival and development of secondary follicles in murine (12, 14), and ovine (17), as well as bovine embryo development (13, 16). Thyroxine concentrations usually used in the experiments with preantral follicles vary from 0.5 µg /ml to 2 µg/ml (15, 17-20). Nevertheless, even though few in vitro studies reported the effects of TH on the development of late preantral follicle categories to the best of our knowledge of its in vitro role on primordial follicle survival and development remains unknown.…”

Section: Introductionmentioning

confidence: 99%

The subtle balance of insulin and thyroxine on survival and development of primordial follicles culturedin vitroenclosed in ovarian tissue

Paes

Lima

Ferreira

et al. 2019

Preprint

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23Thyroid hormones have presented a positive hormonal interaction on follicular 24 development of secondary follicles and oocytes from antral follicles; however, the effect 25 of thyroid hormones on primordial follicles is unclear. Here we investigated the in vitro 26 effects of combined insulin and thyroxine on caprine primordial follicle survival and 27 development. Ovarian tissues were cultured for 1 or 7 days using 10 ng/ml (low) or 10 28 µg/ml (high) insulin in the absence or presence of thyroxine at 0.5, 1 or 2 µg/ml. 29Thereafter, follicular survival and development, gene expression related to apoptosis 30 (Bcl2/Bax), insulin and thyroid receptors, and estradiol and reactive oxygen species 31 production were evaluated. In low-insulin conditions, supplementation with 2 µg/ml 32 thyroxine maintained follicular survival similar to non-cultured control, while 0.5 µg/ml 33 thyroxine enhanced the survival (P<0.05) in comparison to thyroxine-free treatment. Only 34 treatments containing low-insulin and thyroxine at 0.5 or 2 µg/ml increased (P<0.05) 35 reactive oxygen species production from day 1 to day 7. Contrarily to high-insulin 36 containing medium, the presence of thyroxine in low-insulin medium yielded higher 37 stromal cell density (P<0.05). There were higher (P<0.05) estradiol production and 38 Bcl2/Bax ratio in low-insulin versus high-insulin treatments on day 1 and 7, respectively. 39High levels of both insulin and thyroxine showed better follicular development (P<0.05), 40 yielding great follicle and oocyte diameter. Finally, the high-insulin level led to insulin 41 and thyroid receptors expression reduction as compared to non-cultured control. In 42 conclusion, the combination of low concentrations of insulin and thyroxine better 43 maintained follicle survival, while high levels ensured better follicular development. 44 45 3 46 65 20). Nevertheless, even though few in vitro studies reported the effects of TH on the 66 development of late preantral follicle categories to the best of our knowledge of its in 67 vitro role on primordial follicle survival and development remains unknown. 68 Among the various hormones that act on folliculogenesis, insulin has an important 69 role in follicle development due to its action on cell survival and proliferation (21). Our 4 70team has shown that insulin effects could be influenced by follicular category (22, 23), as 71 well as its interactions with other substances (24)(25)(26). For instance, the use of insulin at 72 10 ng/ml during in vitro culture of either primordial (22), secondary or tertiary follicles 73 (23) yielded greater oocyte growth than using 10 µg/ml insulin. On the other hand, 74 secondary follicles cultured in vitro with insulin at 10 µg/ml in association to FSH (24) 75 and GH (25), or FSH + VEGF (26) enhance meiosis resumption in goat oocytes. 76Although the hormonal network is a complex process, the balance of insulin and 77 thyroxine concentration on in vitro primordial follicles development is unclear. 78Therefore, the novelty of the...

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Effects of thyroid hormone on the GH signal transduction pathway

Ocaranza

Lammoglia

Íñiguez

et al. 2014

Growth Hormone & IGF Research

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Effects of thyroxin (T4) and activin A on in vitro growth of preantral follicles in domestic cats

Cited by 16 publications

References 43 publications

Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model

Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model

The subtle balance of insulin and thyroxine on survival and development of primordial follicles culturedin vitroenclosed in ovarian tissue

Effects of thyroid hormone on the GH signal transduction pathway

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