Effects of thioredoxin on the preimplantation development of bovine embryos

Bing, Yinzhong; Hirao, Yuji; Takenouchi, Naoki; Che, Limei; Nakamura, Hajime; Yodoi, J; Nagai, Takashi

doi:10.1016/s0093-691x(02)01158-5

Cited by 27 publications

(22 citation statements)

References 49 publications

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“…TXN helps the embryo to resist against oxidative stress (Kobayashi-Miura et al 2002). For instance, supplementation of the culture medium with thioredoxin was found to improve blastocyst formation, cell numbers, and incidence of apoptosis of in vitroproduced embryos of pigs (Ozawa et al 2006), cattle (Bing et al 2003), and mice (Nonogaki et al 1991). Lopata et al (2001) demonstrated the importance of …”

Section: Discussionmentioning

confidence: 99%

Bovine blastocysts with developmental competence to term share similar expression of developmentally important genes although derived from different culture environments

Ghanem¹,

Salilew‐Wondim²,

Gad³

et al. 2011

Reproduction

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This study was conducted to investigate the gene expression profile of in vivo-derived bovine embryo biopsies based on pregnancy outcomes after transferring to recipients. For this, biopsies of 30-40% embryos were taken from grade I blastocysts (International Embryo Transfer Society Manual) and the remaining 60-70% of the intact embryos were transferred to recipients. Frozen biopsies were pooled into three distinct groups based on the pregnancy outcome after transferring the corresponding parts, namely those resulting in no pregnancy (NP), pregnancy loss (PL), and calf delivery (CD). Array analysis revealed a total of 41 and 43 genes to be differentially expressed between biopsies derived from blastocysts resulting in NP versus CD and PL versus CD respectively. Genes regulating placental development and embryo maternal interaction (PLAC8) were found to be upregulated in embryo biopsies that ended up with CD. Embryo biopsies that failed to induce pregnancy were enriched with mitochondrial transcripts (Fl405) and stress-related genes (HSPD1). Overall, gene expression profiles of blastocysts resulting in NP and CD shared similar expression profiles with respect to genes playing significant roles in preimplantation development of embryo. Finally, comparing the transcript signatures of in vivo-and in vitro-derived embryos with developmental competence to term revealed a similarity in the relative abundance of 18 genes. Therefore, we were able to present a genetic signature associated with term developmental competence independent of the environmental origin of the transferred blastocysts.

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Section: Discussionmentioning

confidence: 99%

Bovine blastocysts with developmental competence to term share similar expression of developmentally important genes although derived from different culture environments

Ghanem¹,

Salilew‐Wondim²,

Gad³

et al. 2011

Reproduction

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“…In somatic cells, the overexpression of H6PD induces the expression of prooxidative enzymes (Leopold et al 2003, Park et al 2006, which is consistent with the overexpression of thioredoxin (TXN) in our parthenotes. The ubiquitous protein disulfide reductase TXN plays a role in the mouse implantation (Nonogaki et al 1991), and responds to oxidative stress in bovine and mouse embryos (Nonogaki et al 1991, Bing et al 2003. The TXN gene is downregulated in embryos that fail to give pregnancies and those leading to resorption (El-Sayed et al 2006), while the addition of the compound to a pig embryo culture improves blastocyst formation, cell numbers, and apoptosis (Ozawa et al 2006).…”

Section: Development and Gene Expression In Parthenotesmentioning

confidence: 99%

Biological differences between in vitro produced bovine embryos and parthenotes

Gómez¹,

Gutiérrez‐Adán²,

Díez³

et al. 2009

Reproduction

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Parthenotes may represent an alternate ethical source of stem cells, once biological differences between parthenotes and embryos can be understood. In this study, we analyzed development, trophectoderm (TE) differentiation, apoptosis/necrosis, and ploidy in parthenotes and in vitro produced bovine embryos. Subsequently, using real-time PCR, we analyzed the expression of genes expected to underlie the observed differences at the blastocyst stage. In vitro matured oocytes were either fertilized or activated with ionomycin C6-DMAP and cultured in simple medium. Parthenotes showed enhanced blastocyst development and diploidy and reduced TE cell counts. Apoptotic and necrotic indexes did not vary, but parthenotes evidenced a higher relative proportion of apoptotic cells between inner cell mass and TE. The pluripotence-related POU5F1 and the methylation DNMT3A genes were downregulated in parthenotes. Among pregnancy recognition genes, TP-1 was upregulated in parthenotes, while PGRMC1 and PLAC8 did not change. Expression of p66 shc and BAX/BCL2 ratio were higher, and p53 lower, in parthenotes. Among metabolism genes, SLC2A1 was downregulated, while AKR1B1, PTGS2, H6PD, and TXN were upregulated in parthenotes, and SLC2A5 did not differ. Among genes involved in compaction/blastulation, GJA1 was downregulated in parthenotes, but no differences were detected within ATP1A1 and CDH1. Within parthenotes, the expression levels of SLC2A1, TP-1, and H6PD, and possibly AKR1B1, resemble patterns described in female embryos. The pro-apoptotic profile is more pronounced in parthenotes than in embryos, which may differ in their way to channel apoptotic stimuli, through p66 shc and p53respectively, and in their mechanisms to control pluripotency and de novo methylation.Reproduction (2009) 137 285-295

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“…The consequences of such damage alter the mitochondrial functions, inhibit embryonic cell division, deplete adenosine triphosphatase, and finally result in apoptosis (programmed cell death) (Kowaltowski and Vercesi, 1999;Hyslop et al, 1988;Yang et al, 1998). Therefore, several antioxidants, such as thioredoxin (Bing et al, 2003), ␤-mercaptoethanol, cysteine (de Matos and Furnus, 2000), cysteamine (Kobayashi et al, 2006), anthocyanin (You et al, 2010), and melatonin (Wang et al, 2013;Gao et al, 2012), have been added to in vitro maturation (IVM) and in vitro culture (IVC) systems to improve the maturation of oocytes and the developmental competence of pre-implantation blastocysts.…”

Section: Introductionmentioning

confidence: 99%