Effects of Thidiazuron and Nutrient Salt Formulations on Micropropagation of Hardy Hibiscus (Hibiscus Moscheutos L.)

West, Todd P.; Preece, John E.

doi:10.17660/actahortic.2004.630.36

Cited by 12 publications

(11 citation statements)

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“…Scoville and West tested different types of nutrient salt formulations and their initial results indicated that axillary shoot initiation of H. rosa-sinensis L. was better at high nutrient salt formulation in combination with low phenyl-N 1 -1,2,3,-thiadiazol-5-ylurea (TDZ) concentrations. This corresponds to reports in H. moscheutos L. where the low nutrient salt McCown Woody Plant Medium (WPM) (Lloyd and McCown 1980) did not proliferate axillary shoots rather the primary shoot elongated with no branching, but high nutrient salt medium, on the other hand, was reported to provide satisfactory results (West and Preece 2004). Our preliminary studies showed that extensive chlorosis could be observed in H. rosa-sinenesis L. shoots multiplied on WPM.…”

Section: Introductionsupporting

confidence: 89%

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In vitro culture of Hibiscus rosa-sinensis L.: Influence of iron, calcium and BAP on establishment and multiplication

Christensen

Sriskandarajah

Serek

et al. 2008

Plant Cell Tiss Organ Cult

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Some factors influencing in vitro cultures of potted Hibiscus rosa-sinensis L. using nodal cuttings were investigated. A protocol using a modified MS medium helped to overcome chlorosis, shoot tip necrosis (STN) and leaf drop. These disorders have been caused by mineral imbalance associated with calcium and iron deficiency. STN and leaf drop were overcome by increasing calcium level from 3 mM (MS standard concentration) to 9 mM, and this increase, in addition, enhanced shoot dry weight and shoot extension. The chlorophyll content and leaf area increased by increasing the iron concentration 3-fold from 98 lM to 295 lM. Furthermore, substituting Fe-EDTA with Fe-EDDHA resulted in an increase in chlorophyll content, leaf area and shoot extension. The most suitable multiplication medium for H. rosa-sinensis L. was demonstrated to be a modified MS medium containing 2.2 lM BAP and increased concentrations of calcium at 9 mM and iron at 295 lM provided as Fe-EDDHA. The shoots were rooted in half-strength modified MS medium containing 2.7 lM NAA. Acclimatization was successful with all shoots with or without roots.

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Section: Introductionsupporting

confidence: 89%

“…In vitro methods have been reported for some related species of H. rosa-sinensis L. such as H. cannabinus L. (Zapata et al 1999;Srivatanakul et al 2000;Herath et al 2004), H. moscheutos L. (West and Preece 2004), H. acetosella Welw. ex Hiern (Reynolds and Blackmon 1983) and H. syriacus L. (Yang et al 1995;Jenderek and Olney 2001).…”

Section: Introductionmentioning

confidence: 99%

In vitro culture of Hibiscus rosa-sinensis L.: Influence of iron, calcium and BAP on establishment and multiplication

Christensen

Sriskandarajah

Serek

et al. 2008

Plant Cell Tiss Organ Cult

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“…Nodal explants of the two cultivars were placed in vitro using the methods described by West and Preece (2004). All media contained Driver and Kuniyuki Walnut (DKW) minerals and vitamins (Driver and Kuniyuki 1984), 3% sucrose, and 10 -7 M thidiazuron (TDZ).…”

Section: Aseptic Nutrient Media and Proliferation Stock Culturesmentioning

confidence: 99%

Encapsulation, cold storage, and growth of Hibiscus moscheutos nodal segments

West

Ravindra²,

Preece

2006

Plant Cell Tiss Organ Cult

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Nodal segments of Hibiscus moscheutos (hardy hibiscus) were excised from proliferating axillary shoot cultures and encapsulated in high density sodium alginate hardened by 50 mM CaCl 2 . Nodal segments 4 mm long grew as well as and were easier to encapsulate than 8 mm long nodal segments. Although nodal segments grew regardless of the concentration of sodium alginate, 2.75% was determined to produce the highest quality encapsulated nodal segments beads (sufficient alginate coating and ease of use) because of the viscosity produced by the 2.75% sodium alginate solution. When encapsulated segments were stored at 5°C they did not grow in light or darkness. During the first month on fresh proliferation medium under normal incubation conditions following 5°C storage in the dark for up to 24 weeks, root number and root and shoot elongation were inhibited linearly as storage time increased. All encapsulated nodal segments survived 24 weeks of 5°C storage in two separate experiments. In fact, 80% of encapsulated hardy hibiscus nodal segments survived refrigerated storage for 1½ years (78 weeks) and after 3 months on proliferation medium, the nodal segments produced nearly the same length axillary shoots with the same number of axillary nodes per shoot as compared to encapsulated segments either not stored at 5°C or stored for 24 weeks at 5°C. Growth from encapsulated and cold-stored 'Lord Baltimore' nodal segments was more vigorous than from 'Southern Belle' nodal segments.

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“…The best rooting material should be taken from actively growing stock plants. The response of the clones to growth regulators or propagation by tissue culture techniques (West and Preece, 2004) has not been evaluated.…”

Section: Culturementioning

confidence: 99%

‘Lufkin Red’ and ‘Lufkin White’ Winter-hardy Hibiscus (Hibiscus ×laevis All.)

Pounders¹

2013

horts

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Effects of Thidiazuron and Nutrient Salt Formulations on Micropropagation of Hardy Hibiscus (Hibiscus Moscheutos L.)

Cited by 12 publications

References 0 publications

In vitro culture of Hibiscus rosa-sinensis L.: Influence of iron, calcium and BAP on establishment and multiplication

In vitro culture of Hibiscus rosa-sinensis L.: Influence of iron, calcium and BAP on establishment and multiplication

Encapsulation, cold storage, and growth of Hibiscus moscheutos nodal segments

‘Lufkin Red’ and ‘Lufkin White’ Winter-hardy Hibiscus (Hibiscus ×laevis All.)

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