Effects of thermal disinfection and autoclave sterilisation on the quality of microalgae concentrates

Dan, Shigeki; Ashidate, Masakazu; Yamashita, Takashi; Hamasaki, Katsuyuki

doi:10.1111/are.13822

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…It has been recognized that Artemia nauplii and early metanauplii lack the capability to digest microalgae possessing thick cell walls, such as Chlorella and Nannochloropsis (Dan et al, 2016; Dan, Ashidate, et al, 2018; Dhont & van Stappen, 2003; Lavens & Sorgeloos, 1991; Seixas et al, 2009; Sick, 1976). However, the point at which they become capable of digesting these microalgae remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…The microalgae genera Chlorella and Nannochloropsis have thick cell walls, and therefore, their living cells can be concentrated at high densities of over 10 10 cells ml −1 and are capable of preservation in a refrigerator for months (Camacho‐Rodríguez et al, 2016; Maruyama et al, 2007; Sales et al, 2019). Moreover, their shelf life can be further prolonged using sterilization treatment (Dan, Ashidate, et al, 2018). These genera are capable of large‐scale industrial culturing and have advantages concerning nutritional status; Nannochloropsis oculata contains a rich amount of eicosapentaenoic acid (EPA) more than 20% in total fatty acids in its cells, and the cells of Chlorella vulgaris can be fortified with designated fatty acids, including EPA and docosahexaenoic acid (DHA) (Camacho‐Rodríguez et al, 2016; Hayashi et al, 2001; Kobayashi et al, 2009; Maruyama et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Considering these advantages, concentrates and pastes of C. vulgaris and N. oculata are commercially available mainly as foods for culturing the rotifers of the Brachionus plicatilis species complex and as nutritional enrichment materials, as well as additive agents supplemented into larval rearing water (green water technology) (Bentley et al, 2008; Dhert et al, 2001; Kobayashi et al, 2009; Maruyama et al, 2007; Palmer et al, 2007; Pfeiffer & Ludwig, 2007). However, young Artemia (nauplii and early metanauplii) are not capable of easy digesting C. vulgaris and N. oculata due to their thick cell walls (Dan et al, 2016; Dan, Ashidate, et al, 2018; Dhont & van Stappen, 2003; Lavens & Sorgeloos, 1991; Seixas et al, 2009; Sick, 1976), and the size at which Artemia become capable of digesting these microalgae remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Capability of Artemia to digest Chlorella vulgaris and Nannochloropsis oculata under stagnant culture conditions

Dan

Yamashita

Matsunari

et al. 2022

Aquaculture Research

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Artemia is an important live food for rearing aquatic zooplanktivorous animals (Dhont et al., 2013;Dhont & van Stappen, 2003;Sorgeloos et al., 2001). The size of Artemia franciscana from Great Salt Lake, Utah, USA, which is the most widely adopted strain in hatcheries and laboratories, ranges from 0.4 mm to 12 mm in total length (TL) depending on life stage. This size range is suitable for consumption by larvae and juveniles of various commercially important fish, crustacean and cephalopod species, most of which exhibit aggressive feeding behaviour on Artemia (Dhont et al., 2013;Sorgeloos et al., 2001). Furthermore, a sufficient supply of Artemia can be obtained from commercially available cysts, which can be stored for years at low temperatures. For these reasons, the use of Artemia is essential for the production of juveniles of many species for aquaculture, stock enhancement programmes and the farming of aquatic ornamental species.A number of reports have discussed hatching technologies, culture methods, ontogenetic changes in nutritional status and nutritional enrichment methods of Artemia in terms of improving dietary values for cultured animals (

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Capability of Artemia to digest Chlorella vulgaris and Nannochloropsis oculata under stagnant culture conditions

Dan

Yamashita

Matsunari

et al. 2022

Aquaculture Research

Self Cite

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“…The harvested microalgae were then centrifuged for 10 min at 15°C (Sutkowy & Klosowski, 2018) for both live and digestible microalgae. For the preparation of digestible microalgae, the concentrated microalgae were autoclaved at 121 °C and 0.2 Mpa for 15 min (Dan et al, 2018).…”

Section: Microalgae Culturementioning

confidence: 99%

Effects of rotifer and Artemia enrichment in the first feeding and larval morphogenesis of purple mud crab, Scylla tranquebarica larvae

Sanudin

Thien

Hamasaki

et al. 2022

Aquaculture Research

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The effects of feeding rotifers and Artemia enriched with different types of enrichment in the first feeding and later stages of Scylla tranquebarica larvae were investigated. Two different experiments were conducted each with five enrichment treatments including live and digestible Nannochloropsis sp. and Tetraselmis sp. or commercial product. In Experiment I, the zoea 1 (Z1) larvae were fed with enriched or unenriched rotifers as their first feed until they moulted into the zoea 2 (Z2) stage, while in Experiment II, the zoea 3 (Z3) larvae were fed with enriched or unenriched Artemia until they metamorphosed into megalopa stage. In Experiment I, Z1 fed enriched rotifers showed higher survival, larval stage index (LSI) and carapace length than those in unenriched treatment. However, the highest survival was recorded in larvae fed Nannochloropsis sp. treatment. In Experiment II, Z3 fed Artemia enriched with digestible Tetraselmis sp. exhibited the highest survival and LSI, lowest chela‐carapace length ratio and abnormal moulting frequencies. In comparison to live microalgae, digestible treatment resulted in a smaller chela‐carapace length ratio with lower abnormal moulting. In conclusion, enrichments tested in this study can be used to enrich rotifers for the first feeding of Z1, while later stage (Z3 onwards) fed Artemia enriched with digestible Tetraselmis sp. improved survival and decreased the abnormal moulting frequencies of Z5 to megalopa stage.

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Chlorella in aquaculture: challenges, opportunities, and disease prevention for sustainable development

Aly

ElBanna²,

Fathi

2023

Aquacult Int

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Aquaculture is the farming of aquatic organisms like fish, crustaceans, mollusks, and aquatic plants, which has become a crucial source of protein and income. However, bacterial infections pose a significant challenge to the aquaculture industry and traditional treatments, such as antibiotics and chemicals, have limitations and environmental concerns. Disease prevention and control measures, such as the use of probiotics, vaccines, and biosecurity measures, are essential for the sustainable development of the aquaculture industry. Further research is also needed to develop more effective and sustainable strategies for the prevention and control of bacterial fish pathogens in aquaculture, where alternative treatments such as herbal extracts, essential oils, and probiotics require further investigation for efficacy and safety. Microalgae, particularly Chlorella, have potential applications in various industries such as biofuels, pharmaceuticals, and wastewater treatment. However, their large-scale production and commercialization face challenges. Safety of Chlorella to fish is a crucial issue that requires careful evaluation, with hematology being an essential tool to assess its effects on fish health and physiology. Studies show that Chlorella is safe for fish and does not have adverse effects on growth, survival, or immune system function. Chlorella is a safe and sustainable option for aquaculture, free from harmful chemicals and antibiotics. The Green Water System utilizes Chlorella as a natural filter and nutrient recycler, improving water quality and providing a well-balanced diet for aquatic animals. This eco-friendly approach also enhances fish immune systems, growth rates, and survival rates. The scientometric review shows significant research activity, with Chang JS being a prominent author and People’s R China and the Chinese Academy of Sciences leading in contributions. The use of Chlorella shows promise as an alternative treatment for bacterial fish pathogens in aquaculture due to its antibacterial properties, safety, and sustainability. However, challenges such as cost-effectiveness and standardization need to be addressed for successful implementation in the aquaculture industry.

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Effects of thermal disinfection and autoclave sterilisation on the quality of microalgae concentrates

Cited by 4 publications

References 36 publications

Capability of Artemia to digest Chlorella vulgaris and Nannochloropsis oculata under stagnant culture conditions

Capability of Artemia to digest Chlorella vulgaris and Nannochloropsis oculata under stagnant culture conditions

Effects of rotifer and Artemia enrichment in the first feeding and larval morphogenesis of purple mud crab, Scylla tranquebarica larvae

Chlorella in aquaculture: challenges, opportunities, and disease prevention for sustainable development

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