Effects of Therapeutic Ultrasound on the Regeneration of Skeletal Myofibers After Experimental Muscle Injury

Rantanen, Jussi; Thorsson, Ola; Wollmer, Per; Hurme, Timo; Kalimo, Hannu

doi:10.1177/03635465990270011701

Cited by 134 publications

(104 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies have shown that low-intensity US (LIUS) is effective in regenerating injured tissues, including muscles (3,4) and bones (5)(6)(7)(8)(9). In particular, LIUS has been shown to enhance the healing of fractured bones by inducing chondrocyte proliferation (7,9).…”

mentioning

confidence: 99%

Effects of low‐intensity ultrasound (LIUS) stimulation on human cartilage explants

Min

Woo²,

Cho

et al. 2006

Scandinavian Journal of Rheumatology

View full text Add to dashboard Cite

Objective: To evaluate the effects of low-intensity ultrasound (LIUS) stimulation on the anabolic state of human cartilage from patients with osteoarthritis (OA). Methods: Explant cultures of human OA cartilage were stimulated for 10 min every day for 7 consecutive days using continuous-wave sonication at a frequency of 1 MHz with spatial and temporal average intensities of 0 (control), 40, 200, 500, or 700 mW/cm 2 . The effects of LIUS on cell proliferation were evaluated by 3 H-thymidine incorporation. Proteoglycan synthesis was evaluated by the incorporation of 35 S-sulfate and by Safaranin O staining. Collagen synthesis was evaluated by 3 H-proline incorporation and immunohistochemistry. Results: At an intensity of 200 mW/cm 2 , LIUS treatment induced the expression of collagen type II and proteoglycan measured by the incorporation of radioactivity and specific staining of the cartilage explants. However, the expression decreased again at the higher intensities of 500 or 700 mW/cm 2 . Ultrasound had no stimulatory effect on cell proliferation at any intensity. Conclusion: LIUS has anabolic effects on human cartilage in explant cultures, indicating a potentially important method for the repair of osteoarthritic cartilage.Osteoarthritis (OA) is the most common joint disease and is characterized by progressive joint destruction, ultimately leading to the need to replace major joints. Although the precise pathological mechanism has not yet been defined, changes in the function of the chondrocytes are thought to be the main cause of the pathology of OA, particularly the imbalance between the anabolism and the catabolism of the extracellular matrix produced from chondrocytes. Methods to increase synthesis of the cartilage matrix and inhibit its degradation may therefore provide effective treatments for OA.Ultrasound (US) has been used extensively in diagnostic applications (1, 2) and there has recently been increasing interest in its therapeutic use for various musculoskeletal disorders. Several studies have shown that low-intensity US (LIUS) is effective in regenerating injured tissues, including muscles (3, 4) and bones (5-9). In particular, LIUS has been shown to enhance the healing of fractured bones by inducing chondrocyte proliferation (7,9). When applied to a monolayer culture of human chondrocytes, LIUS also increased cellular proliferation and matrix synthesis (10). In animal studies, LIUS stimulation in both bone fractures and cartilage resulted in increased expression of proteoglycan or type II collagen rather than that of bone formation factors such as transforming growth factor-b (TGF-b), osteocalcin, alkaline phosphatase, and a1(I)-procollagen, hence protecting the collagen matrix and preventing the destruction or degradation of cartilage (11-13). However, the effects of LIUS treatment on chondrocyte proliferation and the expression of matrix proteins such as proteoglycan and collagen type II have not been clearly shown in intact human cartilage.In this study, we evaluated the LIUS effects on the ana...

show abstract

mentioning

confidence: 99%

Effects of low‐intensity ultrasound (LIUS) stimulation on human cartilage explants

Min

Woo²,

Cho

et al. 2006

Scandinavian Journal of Rheumatology

View full text Add to dashboard Cite

show abstract

“…Although there is no promising animal or clinical evidence that US can help repair muscle injuries [20][21][22][23] , there is plenty of evidence showing the effects of US in collagen repair and re-synthesis 25,[33][34][35][36][37][38][39][40][41] . The use of US in the present clinical case was oriented at improving muscle collagen extracellular matrix (CEM) repair, based upon US effects on fibroblast proliferation and collagen synthesis 25,39) .…”

Section: Discussionmentioning

confidence: 99%

“…Although therapeutic ultrasound (US) is normally recommended for muscle skeletal rehabilitation 20,21) , the scientific evidence from animal studies is not promising 20,22,23) . However, these previous studies focused on myofibrillar changes rather than fibroelastic components such as the muscle extracellular collagen matrix, which has an important role in muscle force production 6,7) .…”

Section: Introductionmentioning

confidence: 99%

“…However, these previous studies focused on myofibrillar changes rather than fibroelastic components such as the muscle extracellular collagen matrix, which has an important role in muscle force production 6,7) . From this viewpoint, there is evidence that mechanical stimuli like therapeutic ultrasound 22,24,25) and early mobilization 13,16) may enhance the muscle injury recovery, stimulating fibroblast metabolism.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Controlled Re-Injury of a Thigh Muscle Tear in a Soccer Player: a Case Study

Gurovich

2012

J Phys Ther Sci

View full text Add to dashboard Cite

Abstract.[Purpose] This case study describes an interesting therapeutic approach to an athlete's muscle injury, which was erroneously diagnosed and treated for the first 48 hours.[Case] During a regular season match, a 17 yearold male, soccer player was hit on his front thigh while he was kicking the ball. The first diagnosis was a contusion, and the player was treated with warm-packs for 48 hours. Ultrasound images revealed a massive muscle tear on the left vastus intermedius of the quadriceps muscle 60 hours after injury. The rehabilitation approach was based on three therapeutic principles: 1) to prevent inflammatory secondary damage, we performed a controlled re-injury to start with a new acute injury; 2) use of physical agents as acute control of the inflammation process; and 3) overlapping rehabilitation phases, such as rest, pain control, healing process, muscle strengthening and aerobic training, to reduce total rehabilitation time.[Results] Weekly ultrasound images showed a total repair after 4 weeks of treatment. Due to the effectiveness of the rehabilitation program the athlete was able to play competitively 35 days after the injury and no re-injury was observed over a 1 year follow-up.[Conclusion] The therapeutic approach used in this case study, which involved the prevention of secondary damage and the overlap of rehabilitation phases, was effective and time efficient.

show abstract

“…In various cell lines ultrasound treatment has been shown to lead to cell proliferation [1,2], enhanced protein activity, enhanced endocytosis [3] and several other biochemical effects [4][5][6]. Some of the beneficial effects of ultrasound upon wound healing have been attributed to inducement of angiogenesis-related cytokine production, increased nitric oxide production and calcium intake [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Ultrasound fails to induce proliferation of human brain and mouse endothelial cell lines

Rodemer¹,

Jenne²,

Fatar³

et al. 2012

AIP Conference Proceedings

View full text Add to dashboard Cite

Abstract. Both in vitro and in vivo studies suggest that ultrasound (US) is capable of inducing angiogenesis. There is no information, however, on whether ultrasound can induce proliferation of brain endothelial cells. We therefore explored the angiogenic potential of ultrasound on a novel immortalised human brain endothelial cell line (hCMEC/D3) and on mouse brain microvascular endothelial cells (bEND3). Ultrasound failed to enhance cell proliferation in both cell lines at all acoustic pressures studied. Endothelial cell damage occurred at 0.24 MPa with significantly slower proliferation. Cells growing in Opticell™ dishes did not show damage or reduced proliferation at these pressures.

show abstract

Effects of Therapeutic Ultrasound on the Regeneration of Skeletal Myofibers After Experimental Muscle Injury

Cited by 134 publications

References 26 publications

Effects of low‐intensity ultrasound (LIUS) stimulation on human cartilage explants

Effects of low‐intensity ultrasound (LIUS) stimulation on human cartilage explants

Controlled Re-Injury of a Thigh Muscle Tear in a Soccer Player: a Case Study

Ultrasound fails to induce proliferation of human brain and mouse endothelial cell lines

Contact Info

Product

Resources

About