Effects of the type of dietary fat on acetylcholine-evoked amylase secretion and calcium mobilization in isolated rat pancreatic acinar cells

Yago, M. D.; Diaz, Ricardo J.; Martínez, M.a C. López; Audi, Nama’a; Naranjo‐Páramo, José; Martínez-Victoria, Emilio; Mañas, Mariano

doi:10.1016/j.jnutbio.2005.04.009

Cited by 5 publications

(4 citation statements)

References 40 publications

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“…At a concentration of 100 nM, ACh stimulated amylase secretion; this ACh-induced amylase release was blocked by pretreatment of acini with atropine (a muscarinic receptor antagonist; 1 and 10 μM; Fig. S2) confirming previously published reports (14,15). Coculture of rat acini (2 million acinar cells) with human PSCs (150,000 cells) significantly stimulated amylase secretion by acini (Fig.…”

Section: Resultssupporting

confidence: 87%

Pancreatic stellate cells produce acetylcholine and may play a role in pancreatic exocrine secretion

Phillips

Yang

Shulkes

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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The pancreatic secretagogue cholecystokinin (CCK) is widely thought to stimulate enzyme secretion by acinar cells indirectly via activation of the vagus nerve. We postulate an alternative pathway for CCK-induced pancreatic secretion. We hypothesize that neurally related pancreatic stellate cells (PSCs; located in close proximity to the basolateral aspect of acinar cells) play a regulatory role in pancreatic secretion by serving as an intermediate target for CCK and secreting the neurotransmitter acetylcholine (ACh), which, in turn, stimulates acinar enzyme secretion. To determine whether PSCs (i) exhibit CCK-dependent ACh secretion and (ii) influence acinar enzyme secretion, primary cultures of human and rat PSCs were used. Immunoblotting and/or immunofluorescence was used to detect choline acetyltransferase (ACh synthesizing enzyme), vesicular ACh transporter (VAChT), synaptophysin, and CCK receptors 1 and 2. Synaptic-like vesicles in PSCs were identified by EM. ACh secretion by PSCs exposed to 20 pM CCK was measured by LC-MS/MS. Amylase secretion by acini [pretreated with and without the muscarinic receptor antagonist atropine (10 μM) and cocultured with PSCs] was measured by colorimetry. PSCs express ACh synthesizing enzyme, VAChT, synaptophysin, and CCK receptors; exhibit CCK-dependent ACh secretion; and stimulate amylase secretion by acini, which is blocked by atropine. In conclusion, PSCs express the essential elements for ACh synthesis and secretion. CCK stimulates ACh secretion by PSCs, which, in turn, induces amylase secretion by acini. Therefore, PSCs may represent a previously unrecognized intrapancreatic pathway regulating CCK-induced pancreatic exocrine secretion.acinar cells | cholecystokinin | pancreas T he pancreas is the major source of the body's digestive enzymes (1). The functional unit of the exocrine pancreas that produces these enzymes is the acinus, composed of individual acinar cells surrounding a central lumen. Acinar cells are capable of synthesizing and secreting as many as 10 million protein (enzyme) molecules per day. The process of enzyme synthesis and secretion at the acinar cell level is highly regulated and involves enzyme synthesis on the rough endoplasmic reticulum, posttranslational changes in the Golgi apparatus, and packaging into zymogen granules. Enzyme secretion is accomplished by the movement of zymogen granules to the apical pole of the acinar cell, fusion of the zymogen granule membrane with the apical plasma membrane followed by discharge of zymogen granule contents into the lumen, a process termed exocytosis (2).Postprandial secretion of pancreatic enzymes is predominantly mediated by regulatory peptides such as cholecystokinin (CCK) and by the vagovagal reflex, which activates cholinergic postganglionic neurons in the gland (3). CCK is known to be a major secretagogue and a trophic factor for the pancreas (4). It is synthesized in I cells of the small intestine and is released into the circulation following a meal. CCK is also present in neurons of the gastrointest...

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Section: Resultssupporting

confidence: 87%

Pancreatic stellate cells produce acetylcholine and may play a role in pancreatic exocrine secretion

Phillips

Yang

Shulkes

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…Overall, the observed changes in the fatty acid composition of pancreatic plasma membranes of HCHOL animals may have occurred as part of a homeostatic mechanism to control membrane fluidity following overload of SFA and cholesterol. In previous experiments in rats, we found that pancreatic plasma membranes show a marked enrichment with those fatty acids most abundant in the diet (Yago et al, 2004(Yago et al, , 2006. This did not happen in the present study in rabbits, probably because of the above-mentioned compensatory mechanism.…”

Section: Discussioncontrasting

confidence: 67%

“…Pancreatic enzyme synthesis and secretion is regulated by a complex integration of stimulatory and inhibitory hormones whose plasma levels are modified by dietary fat (Serrano et al, 1997;. A second, more direct mechanism has been subsequently suggested by our results showing that chronic intake of diets containing different fats can change the responsiveness of the pancreas to pancreatic secretagogues such as cholecystokinin or acetylcholine, in association with profound modification of pancreatic membrane lipid composition (Yago et al, , 2006.…”

Section: Introductionmentioning

confidence: 68%

Effects of a Western-style diet high in cholesterol and saturated faton the rabbit exocrine pancreas

Burgos

Torregrosa²,

Garcãa³

et al. 2015

Turk J Biol

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Despite the well-known cardiovascular effects of an atherogenic diet, there are no data relating to its effect on the exocrine pancreas. Therefore, the aim of this study was to investigate the consequences of this eating pattern on the exocrine pancreas. Twelve New Zealand rabbits were randomised to one of two dietary groups, control group (C) and hypercholesterolemic (HCHOL), that were fed for 50 days with a standard chow or a diet containing 95.7% standard chow, 3% lard, and 1.3% cholesterol, respectively. Pancreatic enzyme activity, cholesterol, and membrane fatty acids were determined by established methods. The activity of pancreatic lipase (6.46 ± 0.948 vs. 1.40 ± 0.460; P < 0.05), colipase (8.93 ± 2.138 vs. 2.36 ± 0.512; P < 0.05), and chymotrypsin (18.54 ± 3.125 vs. 9.69 ± 1.363; P < 0.05) were greater in group HCHOL than in group C. The HCHOL diet increased monounsaturated fatty acids and decreased saturated fatty acids in pancreatic plasma membranes compared with the standard chow. These results suggest a homeostatic adaptation of the pancreas to an atherogenic diet and a greater resistance to the development of lesions than exists in other organs. Further studies are needed, given the lack of research on this issue.

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“…Surprisingly, we found that, in spite of the wide usage in recent years, no information was available on the baseline lipid composition of AR42J cell membranes, and there were no attempts made in recorded scientific literature to modify the fatty acid composition of AR42J cells in culture. Our aim was then to determine the membrane fatty acid composition of AR42J cells, to investigate whether these cells adapt their membranes after exposure to different fatty acids in the culture medium, to confirm if this process is similar to the adaptation of the rat exocrine pancreas that occurs when dietary fat intake is modified (Yago et al, 2006) and if this adaptation is accompanied by a similar modulation of the secretory activity and Ca 2 signaling.…”

Section: Ar42j Studiesmentioning

confidence: 99%

Effects of Olive Oil on Fatty Acid Composition of Pancreatic Cell Membranes

Yago

Martínez

Pariente

et al. 2010

Olives and Olive Oil in Health and Disease Prevention

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Effects of the type of dietary fat on acetylcholine-evoked amylase secretion and calcium mobilization in isolated rat pancreatic acinar cells

Cited by 5 publications

References 40 publications

Pancreatic stellate cells produce acetylcholine and may play a role in pancreatic exocrine secretion

Pancreatic stellate cells produce acetylcholine and may play a role in pancreatic exocrine secretion

Effects of a Western-style diet high in cholesterol and saturated faton the rabbit exocrine pancreas

Effects of Olive Oil on Fatty Acid Composition of Pancreatic Cell Membranes

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