Effects of the topical application of an insect growth regulator (fenoxycarb) on some physiological parameters in the fifth instar larvae of the silkworm Bombyx mori

Leonardi, M.G.; Cappellozza, Silvia; Ianne, P; Cappellozza, L.; Parenti, Paolo; Giordana, B.

doi:10.1016/0305-0491(95)02051-9

Cited by 38 publications

(28 citation statements)

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“…Acute toxicity effects like loss of balance, tremors and extrusion of haemolymph immediately after topical application were consistent with earlier observations made in Leptinotarsa decemlineata Say, 1824 and Epilachna arivestis Mulsant, 1850 (Aller & Ramsay 1988), Popillia japonica Newman, 1841 (Monthean & Potter 1992) and Harmonia axyridis Pallas, 1773 (Carton et al 2003). The observations on cessation of feeding were in good agreement with the findings of Wing et al (1988) in M. sexta, Silhacek et al (1990) in P. interpunctella, Darvas et al (1992) in S. littura, Smagghe & Degheele (1993) in S. exigua and S. exempta, Leonardi et al (1996) in B. mori, Retnakaran et al (1997) in C. fumiferana. Tremor and loss of balance could be due to blockage of K + channel in nerves and muscles in response to non-steroidal ecdysteroid agonist (Salgado 1992).…”

Section: Discussionsupporting

confidence: 90%

“…Dose-dependent inhibition of larval growth and morphogenesis observed is apparently due to reduced food intake associated with a reduced physiological conversion (Leonardi et al 1996). Similar decreases in weight gain in response to other analogues of RH-2485 were reported by Oberlander et al (1995), Smagghe et al (1995Smagghe et al ( , 1997 and Blackford & Dinan (1997a, b).…”

Section: Discussionsupporting

confidence: 79%

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Effect of RH-2485 on development, metamorphosis and synthesis of major proteins in female silkworm Bombyx mori

2010

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Toxicological data on silkworm Bombyx mori are quite comparable to those of other lepidopteran pest insects, therefore, it is considered as a suitable model for exploring effects of any new synthetic formulations. In this study, female V instar larvae of silk moth B. mori were chosen to evaluate the lethal and sublethal toxicity effects of RH-2485 (methoxyfenozide), a non-steroidal ecdysteroid agonist and to substantiate the ecdysteroid mimicking action of RH-2485 on ovary development, vitellogenin incorporation and egg production in isolated pupal abdomen (IPA). Probit analysis was carried out to find the median lethal dose (LD50) from 96 h cumulative mortality percent. Protein profile of haemolymph, fat body, ovary and eggs were separated in SDS-PAGE. Western blot analysis was carried out to confirm vitellogenin in the ovary. Sublethal effects on feeding, cocoon spinning, pupation, adult emergence and egg production were studied at doses of 1/5 th , 1/10 th and 1/20 th of LD50. Significant changes were observed in all these parameters at all three sublethal doses. The morphological effects were related to underlying biochemical changes by finding the changes in haemolymph, fat body, ovary and egg protein profile. Marked changes were observed in storage proteins (80 kDa) and 30 kDa proteins in the haemolymph at all three sublethal doses. The larvae that escaped the sublethal effects at a dose of 1/20 of LD50 and emerged as adults with malformed wings produced significantly lower number of eggs. The isolated pupal abdomen (IPA) treated with RH-2485 did not metamorphose into adult but the oocyte development and vitellogenesis were normal but the egg precursor processing was incomplete leading to failure in choriogenesis.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 79%

Effect of RH-2485 on development, metamorphosis and synthesis of major proteins in female silkworm Bombyx mori

2010

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“…Increases in these characteristics occurred on larvae treated with up to 1 ng methoprene; above this rate, a decline was verified (Figures 2a, 2b and 2c). These curves are explained in that larvae receiving high rates of juvenile hormone analogue have the feeding rhythm and the consumption rate reduced during the extended feeding period (Leonardi et al, 1996). The maintenance cost of this extended period might have contributed for the decrease of the pupa body weight.…”

Section: Resultsmentioning

confidence: 99%

Development and silk production by silkworm larvae after topical application of methoprene

Miranda

Bortoli

Takahashi

2002

Sci. agric. (Piracicaba, Braz.)

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Juvenile hormone analogues have been tested as insect growth regulators in silkworm (Bombyx mori), seeking an increment of silk production. These chemical products, when applied in small or moderate rates, promote the extension of the last larval instar. To understand the physiologic consequences on silk production by the silkworm strain C115 x N108, the application of methoprene, a juvenile hormone analogue, was performed to evaluate its effects on larval development and silk production. Methoprene was topically applied 48h after the fourth larval ecdysis, on the dorsal integument of the 2nd thoracic segment of the insects, at seven rates between 0 and 20 ng a.i. Methoprene influenced positively the duration of the fifth instar and the weight gain of the insects. The application of 1ng methoprene resulted in the heaviest silkglands, cocoons, shell cocoons and pupae weights. Comparatively to the control, the increment on silk production (approximately 24%) by the use of 1ng methoprene was more accentuated than the corresponding negative effects on the cocooning rate (approximately 12%). Key words: Bombyx mori, JHA, sericulture, juvenile hormone DESENVOLVIMENTO E PRODUÇÃO DE SEDA POR LAGARTAS DO BICHO-DA-SEDA APÓS APLICAÇÃO TÓPICA DE METHOPRENERESUMO: Análogos do hormônio juvenil têm sido testados como reguladores de crescimento de insetos em Bombyx mori, com vistas ao incremento da produção de seda. Estes produtos químicos, quando aplicados em pequenas ou moderadas doses, promovem prolongamento do último ínstar larval. O presente estudo visou o entendimento das conseqüências fisiológicas na produção do bicho-da-seda da linhagem C115xN108 face a aplicação de methoprene, um análogo do hormônio juvenil, através da determinação de seus efeitos sobre o desenvolvimento larval e a produção de seda. Methoprene foi aplicado, por via tópica, 48 horas após a quarta ecdise larval, sobre o tegumento dorsal do segundo segmento torácico dos insetos, em sete doses entre 0 e 20 ng de i.a. Methoprene influenciou positivamente a duração do ínstar e o ganho de peso dos insetos. A aplicação de 1 ng de methoprene resultou nos pesos mais elevados de glândulas sericígenas (1,89 g), casulos cheios (2,36 g), casulos vazios (0,62 g) e pupa (1,74 g). Comparativamente ao controle, o incremento na produção de seda pelo uso de 1 ng de methoprene (aproximadamente 24%) foi mais acentuado que os correspondentes efeitos negativos na taxa de encasulamento (aproximadamente 12%).

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“…Antifeedant Activity. The antifeedant activity of these compounds was also carried out by leaf dip method, 34,35 using fourth instar larvae of Spodoptera litura. The leaf discs of about 25 cm 2 were prepared and dipped for thirty seconds in various test compounds.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of Some New Biologically Active Benzothiazole Derivatives Containing Benzimidazole and Imidazoline Moieties

Chaudhary¹,

Pareek²,

Pareek³

et al. 2011

Bulletin of the Korean Chemical Society

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Synthesis of N-(1H -benzimidazol-2-yl)-6-substituted-1,3-benzothiazol-2-amines and 6-substituted-N-(4,5-dihydro-1H-imidazol-2-yl)-1,3-benzothiazol-2-amines by the reaction of substituted 2-aminobenzothiazoles with carbon disulphide and methyl iodide followed by the reaction with o-phenylene diamine/ethylene diamine are reported. All the synthesized compounds were characterized by elemental analysis, IR spectra and 1 H NMR spectral studies. The potent antibacterial and entomological (antifeedant, acaricidal, contact toxicity and stomach toxicity) activities of the synthesized compounds were investigated.

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Effects of the topical application of an insect growth regulator (fenoxycarb) on some physiological parameters in the fifth instar larvae of the silkworm Bombyx mori

Cited by 38 publications

References 14 publications

Effect of RH-2485 on development, metamorphosis and synthesis of major proteins in female silkworm Bombyx mori

Effect of RH-2485 on development, metamorphosis and synthesis of major proteins in female silkworm Bombyx mori

Development and silk production by silkworm larvae after topical application of methoprene

Synthesis of Some New Biologically Active Benzothiazole Derivatives Containing Benzimidazole and Imidazoline Moieties

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