Effects of the surface modification of poly(amino acid)/hydroxyapatite/calcium sulfate biocomposites on the adhesion and proliferation of osteoblast‐like cells

Fan, Xing; Ren, Haohao; Liu, Pengzheng; Wang, Peng; Hong, Liu; Yan, Yonggang; Lv, Guoyu

doi:10.1002/app.42427

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…The challenge in developing biomaterials for engineered tissue is to create surfaces that can direct new tissue regeneration. 35 Also, the interaction of cells with surface topology has been proved to be a significant signaling modality in regulating cell functions such as cell adhesion, migration, and proliferation. 36 Figure 7 showed the cell proliferation of MC3T3-E1 cells grown on the different scaffolds.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Enhanced in Vitro Mineralization and in Vivo Osteogenesis of Composite Scaffolds through Controlled Surface Grafting of l-Lactic Acid Oligomer on Nanohydroxyapatite

Wang

et al. 2016

Biomacromolecules

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Nanocomposite of hydroxyapatite (HA) surface grafted with L-lactic acid oligomer (LAc oligomer) (op-HA) showed improved interface compatibility, mechanical property, and biocompatibility in our previous study. In this paper, composite scaffolds of op-HA with controlled grafting different amounts of LAc oligomer (1.1, 5.2, and 9.1 wt %) were fabricated and implanted to repair rabbit radius defects. The dispersion of op-HA nanoparticles was more uniform than n-HA in chloroform and nanocomposites scaffold. Calcium and phosphorus exposure, in vitro biomineralization ability, and cell proliferation were much higher in the op-HA1.1 wt %/PLGA scaffolds than the other groups. The osteodifferentiation and bone fusion in animal tests were significantly enhanced for op-HA5.2 wt %/PLGA scaffolds. The results indicated that the grafted LAc oligomer of 5.2 or 9.1 wt %, which formed a barrier layer on the HA surface, prevented the exposure of nucleation sites. The shielded nucleation sites of op-HA particles (5.2 wt %) might be easily exposed as the grafted LAc oligomer was decomposed easily by enzyme systems in vivo. Findings from this study have revealed that grafting 1.1 wt % amount of LAc oligomer on hydroxyapatite could improve in vitro mineralization, and 5.2 wt % could promote in vivo osteogenesis capacity of composite scaffolds.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Enhanced in Vitro Mineralization and in Vivo Osteogenesis of Composite Scaffolds through Controlled Surface Grafting of l-Lactic Acid Oligomer on Nanohydroxyapatite

Wang

et al. 2016

Biomacromolecules

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“…In vitro bioactivity evaluation by Alamar Blue Assay gave the same results as the in vitro gain weight via immersion in SBF. Cell attachment and proliferation depend on the surface chemistry and surface area 51 . The HA/PEEK surface nanocomposite showed higher rate of cell proliferation than that of bare PEEK owing to the existence of HA and higher surface roughness of HA/PEEK nanocomposite.…”

Section: Discussionmentioning

confidence: 99%

Fabrication and in vitro study of 3D novel porous hydroxyapatite/polyether ether ketone surface nanocomposite

et al. 2021

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The unique characteristics of polyether ether ketone (PEEK) including low elastic modulus, high mechanical strength, and biocompatibility have made it an attractive alternative for the metallic biomaterials. However, its bioinert property is always the main concern, which could lead to poor osseointegration and subsequent clinical failure of the implant. Changing the surface structure to porous structure and mixing it with bioactive hydroxyapatite (HA) are the common methods, which could be used to enhance the properties of the PEEK‐based implants. In this study, friction stir processing was utilized for the fabrication of porous HA/PEEK surface nanocomposite. Scanning electron microscopic image of the nanocomposite surface showed nano‐scale roughness of the porous structure. Water contact angle test confirmed the increase in the wettability of the treated specimens. In vitro bioactivity test via simulated body fluid solution, initial cell adhesion, cell proliferation, and cell differentiation assay also confirmed the enhancement in bioactivity of the treated surface in comparison to the bare PEEK. This surface modification method requires no special equipment and would not damage the heat‐sensitive PEEK substrate due to the low temperature used during the fabrication process.

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“…The electrical properties of nanocomposites were measured using a high resistivity meter (Model Hiresta UP, MCP‐HT450, Japan) at a voltage of 250 V. An MTT (3‐[4,5‐Dimethylthiazol‐2‐yl]‐2, 5‐diphenyltetrazolium bromide) assay was used to determine the biocompatibility of nanocomposites by evaluating cell proliferation and morphology as described in a previous study with tissue culture plate (TCP) as a control .…”

Section: Methodsmentioning

confidence: 99%

Poly(amino acid)/carbon nanotube nanocomposites with enhanced thermal, electrical, and mechanical properties

2018

Self Cite

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Poly(amino acid) (PAA)‐based nanocomposites were synthesized by in situ melting polycondensation with various carbon nanotube (CNT) loadings; the morphology, thermal, mechanical, electrical, and biocompatible properties of the resulting nanocomposites were investigated and characterized. The nanotubes were dispersed within the polymer matrix without the need for coupling agents or surfactants. The crystallization temperature showed a gradual rise with increasing CNT loading, confirming that the nanotubes act as nucleating agents for PAA crystallization. The nanotubes increased the thermal stability of the matrix, leading to an increase in the initial degradation temperature (Ti) and the maximum degradation temperature (Tm). The mechanical properties were gradually enhanced by increasing the CNT content except for the property of elongation at break, which showed its highest value at 1 wt% CNT content. The nanocomposites showed better electrical and biocompatible properties than CNT‐free PAA, suggesting that the incorporation of CNTs is effective for improving the PAA performance, and the nanocomposites are suitable as performance materials for the manufacture of medical apparatus. POLYM. COMPOS., 39:E1939–E1949, 2018. © 2018 Society of Plastics Engineers

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Effects of the surface modification of poly(amino acid)/hydroxyapatite/calcium sulfate biocomposites on the adhesion and proliferation of osteoblast‐like cells

Cited by 8 publications

References 41 publications

Enhanced in Vitro Mineralization and in Vivo Osteogenesis of Composite Scaffolds through Controlled Surface Grafting of l-Lactic Acid Oligomer on Nanohydroxyapatite

Enhanced in Vitro Mineralization and in Vivo Osteogenesis of Composite Scaffolds through Controlled Surface Grafting of l-Lactic Acid Oligomer on Nanohydroxyapatite

Fabrication and in vitro study of 3D novel porous hydroxyapatite/polyether ether ketone surface nanocomposite

Poly(amino acid)/carbon nanotube nanocomposites with enhanced thermal, electrical, and mechanical properties

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