Effects of the Seminal Plasma Zinc Content and Catalase Activity on the Semen Quality of Water Buffalo (Bubalus bubalis) Bulls

Abstract

In order to determine zinc and catalase content of seminal plasma in the buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen volume and sperm concentration, gross and progressive motility and viability were evaluated, seminal plasma was then harvested by centrifugation and its zinc content was estimated by atomic absorption spectrophotometer and its catalase activity determined by using a commercial kit. The zinc content of the seminal plasma (Mean +/- SEM) was recorded as 154.40 +/- 1.74 mg L(-1), while, the mean catalase value was 32.00 +/- 0.42 U mL(-1). The mean zinc values was highly correlated with sperm progressive motility and viability and with catalase values (p = 0.000 for all) and also was associated with gross motility (p = 0.020) and negatively with abnormal morphology (p = 0.049). The catalase values were highly associated with sperm progressive motility, viability and zinc content (p = 0.000 for all) and was associated with sperm gross motility (p = 0.024). For further clarification of these correlations, the samples were categorized in three groups of excellent (Ex, >90% motile, n = 33), good (Go, 80-89% motile, n = 15) and moderate (Mo, <79% motile, n = 6) according to their percentage of sperm motility. The mean progressive motility in Ex group was 92.54 +/- 0.51%, in Go group was 81.66 +/- 0.62% and in Mo group was 71.66 +/- 1.05%. The mean zinc and catalase values were recorded as 161.07 +/- 1.63 mg L(-1) and 33.41 +/- 0.34 U mL(-1) in Ex, 146.70 +/- 1.91 mg L(-1) and 31.01 +/- 0.67 in Go and 136.42 +/- 4.97 mg L(-1) and 26.51 +/- 0.87 U mL(-1) in Mo groups. The mean zinc value in Ex group was highly associated with sperm motility, viability and catalase values, in Go group was associated with catalase values and highly associated with sperm abnormal morphology and in Mo group it was highly associations with catalase values only. The mean catalase value in Ex group, was highly associated with sperm motility and viability, in Go group was associated with zinc content and in Mo groups was highly associated with the zinc content. These results show that seminal plasma zinc and catalase content are correlated with semen characteristics and synergistically act to preserve motility and viability of the spermatozoa after ejaculation.

Sperm maturation in dogs: sperm profile and enzymatic antioxidant status in ejaculated and epididymal spermatozoa

“…Actually, the challenges faced by the spermatozoa after ejaculation would require an antioxidant with instantaneous and efficient action such as catalase (Alfonso‐Prieto et al ., ). As a matter of fact, a positive influence of catalase on ejaculated semen has been found in several species (Michael et al ., ; Alavi‐Shoushtari et al ., ; Li et al ., ). In contrast to the low concentrations of catalase found in this study, the activity of GPx was observed in all samples.…”

Effect of zinc and selenium supplementation on antioxidative status of seminal plasma and testosterone, T4and T3level in goat blood serum

“…Dismutation of superoxide radical is done by superoxide dismutase, which contains Zn. Zn supplementation also increases catalase (CAT) in seminal plasma in buffalo (Alavi-shoushtari et al 2009). Se acts as catalytic centre in the active sites of different antioxidative enzymes (Salvatore et al 1996) and functions as cofactor of glutathione peroxidase (GSHPx) system which regulates extra-and intracellular peroxidases (Burk & Hill 1993).…”

Nano Çinko Oksitin Moghani Koç Semeninin Çözdürme Sonrası Değişkenler ve Oksidatif Durumuna Etkisi

“…This is likely to be related to the function of the element in Zn such that Zinc is associated with ATP, plays a role in shrinking and regulating the energy of its phospholipids, and therefore has a direct impact on sperm motility [25] . In a research on buffalo sperm, it has been shown that zinc in the semen plasma has a direct relationship with the survival and progressively motile of sperm [26] . In another study on buffalo sperm, it has been shown that the presence of semen in the plasma directly affects the survival and progressively motile of sperm [26] .…”

“…In a research on buffalo sperm, it has been shown that zinc in the semen plasma has a direct relationship with the survival and progressively motile of sperm [26] . In another study on buffalo sperm, it has been shown that the presence of semen in the plasma directly affects the survival and progressively motile of sperm [26] .…”