Effects of the piezo-tolerance of cultured deep-sea eel cells on survival rates, cell proliferation, and cytoskeletal structures

Koyama, Sumihiro; Kobayashi, Hiromi; Inoue, Akira; Miwa, Tetsuya; Aizawa, Masuo

doi:10.1007/s00792-005-0462-3

Cited by 19 publications

(22 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Introductionmentioning

confidence: 99%

“…parasiticus-derived pectoral fin cells have greater pressure tolerance than conger eel and mouse 3T3-L1 cells (Koyama et al 2005a). Although no mouse and conger eel cells remained alive after being subjected to pressures of 100 and 130 MPa (1 bar = 0.1 MPa) for 20 min, all of the deep-sea fish cells remained alive after being subjected to pressure of 150 MPa (Koyama et al 2005a). Surface-dwelling organism-derived cells including human, mouse, and conger eel cells undergo morphologic changes accompanied by cytoskeletal depolymerization under hydrostatic pressure of greater than 30 MPa for 20 min (Bourns et al 1988;Crenshaw et al 1996;Koyama et al 2001Koyama et al , 2005a.…”

Section: Introductionmentioning

confidence: 99%

“…Using this system, the deep-sea fish Simenchelys parasiticus (habitat depth, 366-2,630 m; Nakabo 2000) captured from an ocean depth of 1,162 m survived under atmospheric pressure for 5 days after gradual, slow decompression (Koyama et al 2003a(Koyama et al , 2003b. Furthermore, we succeeded in the cultivation and freeze-storage of pectoral fin cells from the living deep-sea fish S. parasiticus in salt-enriched L-15 medium supplemented with 20% (v/v) fetal bovine serum (FBS) at atmospheric pressure (Koyama et al 2003a(Koyama et al , 2003b(Koyama et al , 2005a.…”

Section: Introductionmentioning

confidence: 99%

“…Although no mouse and conger eel cells remained alive after being subjected to pressures of 100 and 130 MPa (1 bar = 0.1 MPa) for 20 min, all of the deep-sea fish cells remained alive after being subjected to pressure of 150 MPa (Koyama et al 2005a). Surface-dwelling organism-derived cells including human, mouse, and conger eel cells undergo morphologic changes accompanied by cytoskeletal depolymerization under hydrostatic pressure of greater than 30 MPa for 20 min (Bourns et al 1988;Crenshaw et al 1996;Koyama et al 2001Koyama et al , 2005a. In contrast, deep-sea fish cells undergo reversible actin and tubulin depolymerization when subjected to hydrostatic pressure of 100 MPa (Koyama et al 2005a).…”

Section: Introductionmentioning

confidence: 99%

“…Surface-dwelling organism-derived cells including human, mouse, and conger eel cells undergo morphologic changes accompanied by cytoskeletal depolymerization under hydrostatic pressure of greater than 30 MPa for 20 min (Bourns et al 1988;Crenshaw et al 1996;Koyama et al 2001Koyama et al , 2005a. In contrast, deep-sea fish cells undergo reversible actin and tubulin depolymerization when subjected to hydrostatic pressure of 100 MPa (Koyama et al 2005a). It is not known whether the binding force between deep-sea cytoskeletal proteins contributes to the pressure resistance of the cytoskeletal structure of deep-sea fish cells.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Piezotolerance of the cytoskeletal structure in cultured deep-sea fish cells using DNA transfection and protein introduction techniques

Koyama

Aizawa

2007

Cytotechnology

Self Cite

View full text Add to dashboard Cite

We used DNA transfection and protein introduction techniques to investigate the pressure tolerance of cytoskeletal structures in pectoral fin cells derived from the deep-sea fish Simenchelys parasiticus (habitat depth, 366-2,630 m). The deepsea fish cells have G418 resistance. The cell number increased until day 6 of cultivation and all cells had died by day 35 when cultured in 35-mm Petri dishes in medium containing G418. Enhanced yellow fluorescent protein-tagged human b-actin (EYFP-actin) was stably expressed by 1 in 100,000 deep-sea fish cells. Because almost none of the EYFP-actin was incorporated into actin filaments of the cells, we replaced the relatively large EYFP tag with a chemical fluorescent compound and succeeded in incorporating fluorescently labeled rabbit actins into the deep-sea fish actin filaments. Most of the filament structure in the cells with rabbit actin inserted underwent depolymerization when subjected to pressure of 100 MPa for 20 min, in contrast to control cells. There were no differences in the tubulin filament structure between control cells and deep-sea fish cells with fluorescein-labeled bovine tubulin inserted after the application of pressure ranging from 40 to 100 MPa for 20 min.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Piezotolerance of the cytoskeletal structure in cultured deep-sea fish cells using DNA transfection and protein introduction techniques

Koyama

Aizawa

2007

Cytotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

Cellular responses in marine animals to hydrostatic pressure

Yancey

2020

J Exp Zool Pt A

View full text Add to dashboard Cite

Hydrostatic pressure (HP), increasing by 1 atm per 10 m in the ocean, perturbs many cellular processes, for example, by rigidifying membranes and disturbing protein folding and ligand binding. Membranes can be fluidized to work under high HP by increasing unsaturated fatty acids, for example, docosahexaenoic acid. Over generations, some deep‐sea proteins have evolved intrinsic resistance to HP, but often incompletely. These may be protected from HP with piezolytes, small organic molecules with pressure‐counteracting properties. The key example is the osmolyte trimethylamine N‐oxide (TMAO), which marine fishes and crustaceans accumulates linearly with depth. TMAO can effectively counteract many inhibitory effects of HP on numerous proteins. For short‐term HP stress, cellular stress (transient) and homeostasis (persistent) responses (CSRs, CHRs) remain poorly characterized, but across different taxa of shallow and terrestrial organisms, they include common CSR/CHR mechanisms known for other stressors—heat shock proteins (HSPs), boosted energy metabolism, antioxidants, cellular repair systems. For vertically migrating marine animals, HP stress responses are even more poorly characterized. Some species (e.g., Anguilla silver eel, king crab Lithodes maja, snubnosed eel Simenchelys parasiticus) cope with HP changes in their habitat range by intrinsic adaptations, lipid desaturase activation, and metabolic adjustments, but perhaps not common CSR mechanisms. Such species may have constitutive stress proteins and/or are able to adjust membrane saturation and/or TMAO rapidly with depth. For permanent deep‐sea species, CSR/CHR mechanisms have not been directly tested, but evidence in Mariana Trench amphipods and snailfish suggest that HSP and desaturase genes, and possibly piezolyte synthesis, have undergone habitat‐related selection.

show abstract

Response of marine bacteria to oil contamination and to high pressure and low temperature deep sea conditions

Fasca

Castilho

et al. 2017

MicrobiologyOpen

View full text Add to dashboard Cite

The effect of pressure and temperature on microbial communities of marine environments contaminated with petroleum hydrocarbons is understudied. This study aims to reveal the responses of marine bacterial communities to low temperature, high pressure, and contamination with petroleum hydrocarbons using seawater samples collected near an offshore Brazilian platform. Microcosms containing only seawater and those containing seawater contaminated with 1% crude oil were subjected to three different treatments of temperature and pressure as follows: (1) 22°C/0.1 MPa; (2) 4°C/0.1 MPa; and (3) 4°C/22 MPa. The effect of depressurization followed by repressurization on bacterial communities was also evaluated (4°C/22 MPaD). The structure and composition of the bacterial communities in the different microcosms were analyzed by PCR‐DGGE and DNA sequencing, respectively. Contamination with oil influenced the structure of the bacterial communities in microcosms incubated either at 4°C or 22°C and at low pressure. Incubation at low temperature and high pressure greatly influenced the structure of bacterial communities even in the absence of oil contamination. The 4°C/22 MPa and 4°C/22 MPaD treatments resulted in similar DGGE profiles. DNA sequencing (after 40 days of incubation) revealed that the diversity and relative abundance of bacterial genera were related to the presence or absence of oil contamination in the nonpressurized treatments. In contrast, the variation in the relative abundances of bacterial genera in the 4°C/22 MPa‐microcosms either contaminated or not with crude oil was less evident. The highest relative abundance of the phylum Bacteroidetes was observed in the 4°C/22 MPa treatment.

show abstract

Effects of the piezo-tolerance of cultured deep-sea eel cells on survival rates, cell proliferation, and cytoskeletal structures

Cited by 19 publications

References 28 publications

Piezotolerance of the cytoskeletal structure in cultured deep-sea fish cells using DNA transfection and protein introduction techniques

Piezotolerance of the cytoskeletal structure in cultured deep-sea fish cells using DNA transfection and protein introduction techniques

Cellular responses in marine animals to hydrostatic pressure

Response of marine bacteria to oil contamination and to high pressure and low temperature deep sea conditions

Contact Info

Product

Resources

About