Effects of the phytoestrogen, genistein, and protein tyrosine kinase inhibitor–dependent mechanisms on steroidogenesis and estrogen receptor expression in porcine granulosa cells of medium follicles

Nynca, Anna; Nynca, Joanna; Wąsowska, Barbara; Kolesárová, Adriana; Kołomycka, A.; Ciereszko, Renata E.

doi:10.1016/j.domaniend.2012.07.002

Cited by 21 publications

(23 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genistein (0.5-50 FM) also decreased production of P 4 in granulosa cells from pig medium follicles (NYNCA et al 2013b). This is consistent with our previous reports describing the inhibitory effects of daidzein (NYNCA et al 2009;NYNCA et al 2013a) and biochanin A (NYNCA et al 2013c) on P 4 secretion by cultured granulosa cells isolated from either medium or large follicles of pigs. Similar inhibitory effects of phytoestrogens were shown in other reports, including one describing genistein-induced inhibition of steroidogenesis in human granulosa cells (WHITEHEAD et al 2002).…”

Section: Discussionsupporting

confidence: 90%

“…In the present study, genistein increased the mRNA level of ERâ, but not that of ERá, in luteinized granulosa cells isolated from large porcine follicles. Similar results were demonstrated in granulosa cells harvested from medium follicles (NYNCA et al 2013a). Daidzein, another phytoestrogen, caused a significant increase in mRNA levels of both ERs in granulosa cells from large follicles (NYNCA et al 2009).…”

Section: Discussionsupporting

confidence: 78%

See 1 more Smart Citation

The Effects of Phytoestrogen Genistein on Steroidogenesis and Estrogen Receptor Expression in Porcine Granulosa Cells of Large Follicles

Nynca¹,

Sadowska²,

Orlowska³

et al. 2015

folia biol (krakow)

View full text Add to dashboard Cite

NYNCA A., SADOWSKA A., ORLOWSKA K., JABLONSKA M., CIERESZKO R.E. 2015. The effects of phytoestrogen genistein on steroidogenesis and estrogen receptor expression in porcine granulosa cells of large follicles. Folia Biologica (Kraków) 63: 119-128. Genistein is a biologically active isoflavone with estrogenic or antiestrogenic activity which can be found in a variety of soy products. Since in pigs diet soy is the main source of protein, genistein may affect the reproductive/endocrine systems in these animals. Genistein has been shown to alter porcine ovarian and adrenal steroidogenesis but the mechanism of this action is still not clear. It is known that genistein binds to both estrogen receptor alpha (ERá) and estrogen receptor beta (ERâ), although it has a higher affinity to ERâ. Moreover, this phytoestrogen was demonstrated to posses the activity of protein tyrosine kinase (PTK)inhibitor. The aim of the study was to examine the in vitro effects of genistein on: (1) progesterone (P " ) and estradiol (E ) secretion by porcine luteinized granulosa cells harvested from large follicles, and (2) the mRNA and protein expression of ERá and ERâ in these cells.In addition, to verify the role of PTKdependent mechanisms possibly involved in genistein biological action, we tested the effects of lavendustin C, the nonsteroidal PTK inhibitor, on granulosa cell steroidogenesis. Genistein significantly inhibited P " and did not affect E secretion by porcine luteinized granulosa cells isolated from large follicles. Lavendustin C did not affect basal steroids secretion by examined cells. Genistein did not alter ERá but increased ERâ mRNA levels in the cultured porcine granulosa cells. In contrast to medium follicles, the expression of ERâ protein was unaffected by genistein in granulosa cells of large follicles. To conclude, the soy phytoestrogen genistein acts directly on the porcine ovary to decrease progesterone production and to increase the expression of ERâ mRNA.Moreover, genistein-induced changes in follicular steroidogenesis and granulosal sensitivity to estrogens in pigs may depend on maturity of the follicles.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 78%

The Effects of Phytoestrogen Genistein on Steroidogenesis and Estrogen Receptor Expression in Porcine Granulosa Cells of Large Follicles

Nynca¹,

Sadowska²,

Orlowska³

et al. 2015

folia biol (krakow)

View full text Add to dashboard Cite

show abstract

“…Methyl jasmonate is related to the stimulation of biosynthesis of genisteine and luteoline. These phenolic compounds are known as cancer‐ and osteoporosis‐preventing agents, and also as anti‐inflammatory compounds …”

Section: Resultsmentioning

confidence: 99%

“…These phenolic compounds are known as cancer-and osteoporosis-preventing agents, and also as anti-inflammatory compounds. 28 The biosynthesis of phenolic compounds is mediated by the action of the enzymes phenylalanine ammonium lyase (PAL, EC 4.3.1.5) and tyrosine ammonium lyase (TAL, EC 4.3.1.23), which participate in the shikimic acid pathway. 29 Genes that codify for PAL are induced by the attack of pathogens and/or abiotic factors.…”

Section: Phenolic Compounds Profilementioning

confidence: 99%

Effect of methyl jasmonate, sodium selenate and chitosan as exogenous elicitors on the phenolic compounds profile of broccoli sprouts

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Epidemiologic studies have shown that an increased intake of soy products leads to a decreased risk of hormone-dependent cancer [2,44,45]. Nevertheless, little is known about the effects of genistein or dietary soy on granulosa cell tumors [46]. Although soy was previously shown to induce GCT [18] in one strain of mice (SWR) when administrated a diet containing daidzein and genistein, the mechanism of this phenomenon remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Soy Promotes Juvenile Granulosa Cell Tumor Development in Mice and in the Human Granulosa Cell Tumor-Derived COV434 Cell Line1

Mansouri-Attia

James

Ligon

et al. 2014

Biology of Reproduction

View full text Add to dashboard Cite

Soy attracts attention for its health benefits, such as lowering cholesterol or preventing breast and colon cancer. Soybeans contain isoflavones, which act as phytoestrogens. Even though isoflavones have beneficial health effects, a role for isoflavones in the initiation and progression of diseases including cancer is becoming increasingly recognized. While data from rodent studies suggest that neonatal exposure to genistein (the predominant isoflavone in soy) disrupts normal reproductive function, its role in ovarian cancers, particularly granulosa cell tumors (GCT), is largely unknown. Our study aimed to define the contribution of a soy diet in GCT development using a genetically modified mouse model for juvenile GCTs (JGCT; Smad1 Smad5 conditional double knockout mice) as well as a human JGCT cell line (COV434). While dietary soy cannot initiate JGCT development in mice, we show that it has dramatic effects on GCT growth and tumor progression compared to a soy-free diet. Loss of Smad1 and Smad5 alters estrogen receptor alpha (Esr1) expression in granulosa cells, perhaps sensitizing the cells to the effects of genistein. In addition, we found that genistein modulates estrogen receptor expression in the human JGCT cell line and positively promotes cell growth in part by suppressing caspase-dependent apoptosis. Combined, our work suggests that dietary soy consumption has deleterious effects on GCT development.

show abstract

Effects of the phytoestrogen, genistein, and protein tyrosine kinase inhibitor–dependent mechanisms on steroidogenesis and estrogen receptor expression in porcine granulosa cells of medium follicles

Cited by 21 publications

References 20 publications

The Effects of Phytoestrogen Genistein on Steroidogenesis and Estrogen Receptor Expression in Porcine Granulosa Cells of Large Follicles

The Effects of Phytoestrogen Genistein on Steroidogenesis and Estrogen Receptor Expression in Porcine Granulosa Cells of Large Follicles

Effect of methyl jasmonate, sodium selenate and chitosan as exogenous elicitors on the phenolic compounds profile of broccoli sprouts

Soy Promotes Juvenile Granulosa Cell Tumor Development in Mice and in the Human Granulosa Cell Tumor-Derived COV434 Cell Line1

Contact Info

Product

Resources

About