Effects of the phenylurea herbicide diuron on natural riverine microbial communities in an experimental study

Pesce, Stéphane; Fajon, Céline; Bardot, Corinne; Bonnemoy, Frédérique; Portelli, Christophe; Bohatier, Jacques

doi:10.1016/j.aquatox.2006.03.006

Cited by 80 publications

(65 citation statements)

References 68 publications

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“…In recent years, a few papers have been published reporting the use of the FISH (Fluorescence in situ hybridization) technique, which is based on the use of fluorescent probes to quantify different phylogenetic groups by fluorescence microscopy. This method was used, for example, by Pesce et al (2006), in a study of the effect of the phenylurea herbicide diuron on river microbial communities. However, this method can also be used for the in-situ detection of bacterial strains that are able to degrade herbicides, as for example by Grenni et al (2009).…”

Section: Non-pcr Based Molecular Methodsmentioning

confidence: 99%

Herbicide Contamination of Freshwater Ecosystems: Impact on Microbial Communities

Villeneuve¹,

Larroudé²,

Humbert³

2011

Pesticides - Formulations, Effects, Fate

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Section: Non-pcr Based Molecular Methodsmentioning

confidence: 99%

Herbicide Contamination of Freshwater Ecosystems: Impact on Microbial Communities

Villeneuve¹,

Larroudé²,

Humbert³

2011

Pesticides - Formulations, Effects, Fate

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“…Other studies have been reported that used TTGE from 16S PCR products to monitor pesticide toxicity on natural riverine microbial communities, using either pooled DNA extracts from replicates before PCR (Pesce et al, 2006), or no replication (Pesce et al, 2008). In our study, three or four replicates per treatment were extracted, amplified and separated.…”

Section: Roundup Impact On Prokaryote Communitiesmentioning

confidence: 99%

“…More recently, these methods were successfully applied to detect disturbances in microbial communities exposed to pollution (Petersen et al, 2004;Kostanjšek et al, 2005;Pesce et al, 2006Pesce et al, , 2008.…”

Section: Introductionmentioning

confidence: 99%

Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment

et al. 2008

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The effects of the herbicide Roundup® (glyphosate) on natural marine microbial communities were assessed in a 7 day field experiment using microcosms. Bottles were maintained underwater at 6 m depth, and 10% of their water content was changed every other day.The comparison of control microcosms and surrounding surface water showed that the microcosm system tested here can be considered as representative of the natural surrounding environment. A Temporal Temperature Gradient gel Electrophoresis (TTGE) was run on 16S This study demonstrates that a disturbance was caused to the marine microbial community exposed to 1 µg L -1 Roundup concentration, a value typical of those reported in coastal waters during a run-off event.

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“…Measuring effects on community-level functions in laboratory microcosms can be comparatively straight-forward, following similar approaches as for the tests of effects of PPPs on microbial assemblages of sewage treatment processes and on microbial nitrogen transformation in soil, already included in the EU data requirements. Endpoints have included, for example, respiration, total bacterial production, potential (anaerobic) denitrification and potential (aerobic) nitrification (Svensson and Leonardsson, 1992;DeLorenzo et al, 1999;Laursen and Carlton, 1999;Enrich-Prast, 2006;Pesce et al, 2006;Milenkovski et al, 2010). It can be assumed that various steps and processes involved in nitrogen cycling and mineralisation are highly relevant endpoints for assessing effects of pollutants, since nitrogen is an essential nutrient but also since nitrogen transformation processes are vital in water purification in both the active sludge process and in constructed wetlands.…”

Section: Investigating Community-level Properties Of Sediment Microormentioning

confidence: 99%

“…Nucleic acid-based molecular methods have higher potential to give detailed information on the structure and diversity of microbial communities and specific phylogenetic or functional groups. Thus, investigations of pesticide effects on aquatic microbial assemblages have employed denaturing or temperature gradient gel electrophoresis, terminal restriction fragment length polymorphism, fluorescence in situ hybridisation and, more recently, 454 pyrosequencing (Chinalia and Killham, 2006;Pesce et al, 2006;Stachowski-Haberkorn et al, 2008;Pesce et al, 2009;Tadonléké et al, 2009;Vercraene-Eairmal et al, 2010;Lin et al, 2012;Aguayo et al, 2014;Dimitrov et al, 2014).…”

Section: Investigating Community-level Properties Of Sediment Microormentioning

confidence: 99%

Scientific Opinion on the effect assessment for pesticides on sediment organisms in edge‐of‐field surface water

2015

EFS2

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The EFSA Panel on Plant Protection Products and their Residues (PPR Panel) was tasked to revise the Guidance Document (GD) on Aquatic Ecotoxicology under Council Directive 91/414/EEC (SANCO/3268/2001 rev. 4 (final), 17 October 2002. This scientific opinion of the PPR Panel is the second of three requested deliverables within this mandate. The scientific background for the risk assessment on sediment organisms in edge-of-field surface waters is provided, with reference to benthic ecology and ecotoxicology, available test protocols and current knowledge on exposure and effects of sediment-bound plant protection products (PPPs). The scientific opinion provides approaches on how to derive regulatory acceptable concentrations (RACs) for sediment organisms and exposure to active substances of PPPs and transformation products of these substances, and how to link them in a tiered approach to predicted environmental concentrations (PECs) for the sediment compartment. A list of uncertainties in relation to such approaches is given. SUMMARYSediment Environmental Risk Assessment (ERA) is a combination of exposure and effect assessment. Proposals for these assessments are provided. The opinion describes specific protection goals (SPGs) for sediment-inhabiting organisms based on two options (1) the ecological threshold option (ETO), accepting negligible population effects only, and (2) if applicable, the ecological recovery option (ERO), accepting some population-level effects if ecological recovery takes place within an acceptable time period.Triggers for sediment ecotoxicity testing are proposed together with a decision scheme for when additional testing may be required. The ecotoxicologically relevant concentrations (ERCs) for sediment organisms are proposed, as this will be influenced by the choice of sediment layer, exposure metric and test duration.The current Forum for the co-ordination of pesticide fate models and their use (FOCUS) methodology for surface water does not consider the effect of multi-year applications, which could possibly lead to accumulation of pesticides in sediment. To account for this deficit, a proposed methodology for introducing an accumulation factor that can be used until an updated FOCUS methodology becomes available is presented in this opinion. The accumulation factor can be considered a conservative approach, since it does not include any possible transport processes, such as leaching or volatilisation, which may reduce the accumulation in sediment in the real field situation.A tiered effect assessment approach is proposed for different sediment organisms and how to link regulatory acceptable concentrations (RACs) to predicted environmental concentration (PECs). The assessment of bioaccumulation, biomagnification and secondary poisoning is discussed, as well as the prospect of improving ERA for sediment microorganisms.Transformation products from active substances also may need to be assessed, as well as mixture toxicity of formulations of plant protection products (PPPs). The op...

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Effects of the phenylurea herbicide diuron on natural riverine microbial communities in an experimental study

Cited by 80 publications

References 68 publications

Herbicide Contamination of Freshwater Ecosystems: Impact on Microbial Communities

Herbicide Contamination of Freshwater Ecosystems: Impact on Microbial Communities

Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment

Scientific Opinion on the effect assessment for pesticides on sediment organisms in edge‐of‐field surface water

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