Effects of the non-peptide, tachykinin-receptor antagonists, (±)CP99994 and (±)SR48968, on guinea-pig airways

Ball, D.I.; Wren, G.P.A.; Pendry, Y.D.; Smith, J. Torquil; Piggott, Leslie A.; Sheldrick, R.L.G.

doi:10.1016/0143-4179(93)90128-w

Cited by 4 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(c) Antibodies to the same NK-1R cytosolic region can vary in specificity and affinity. By revealing the precise location and distribution of NK-1R in the lung, selective NK-1R antagonists can be applied more effectively as potential inhibitors of mucus hypersecretion (MEN 11467; Khan et al 2001) and plasma extravasation (CP 99,994;Ball et al 1993), FK 888 (Murai et al 1993). Non-selective antagonists such as FK 224, which are active on both NK-1 and NK-2 receptor sites and can block both plasma extravasation and bronchoconstriction (Regoli et al 1994), may also be useful for respiratory diseases such as asthma or COPD.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Substance P Receptor (Neurokinin-1 Receptor) in Normal Ovine Lung and During the Progression of Bronchopneumonia in Sheep

Grubor

Ramírez-Romero

Gallup

et al. 2004

J Histochem Cytochem.

View full text Add to dashboard Cite

S U M M A R Y Substance P contributes to the physiological homeostasis of pulmonary airways and vasculature. During pneumonia, alterations in substance P production and receptor expression can influence bronchoconstriction and vascular perfusion. The distribution of substance P receptor [neurokinin-1 receptor (NK-1R)] in lungs of normal sheep and sheep with acute (1 day), subacute (15 days), and chronic (45 days) bronchopneumonia caused by Mannheimia haemolytica was determined by immunohistochemistry (IHC). Three rabbit polyclonal antibodies generated to the same cytosolic C-terminal portion of NK-1R (residues 393-407) were tested. NK-1R immunoreactivity was traced in digital images and quantified with IPLAB software. There were no significant differences in NK-1R protein density between normal and infected lambs. Antibody 1 had the broadest distribution and intensity, and stained alveolar septae, smooth muscle cells of airways and vessels, epithelial cells of airways and alveoli, and submucosal glands. When all animals from the study were included, there was a trend towards decreased NK-1R immunoreactivity over time. The work suggests that (a) the density of NK-1R does not change during progression of bacterial ( M. haemolytica ) bronchopneumonia, (b) NK-1R is widely distributed in ovine lung and decreases with age, and (c) antibodies to the same NK-1R cytosolic region can vary in specificity and affinity.

show abstract