Effects of the nitric oxide donor sodium nitroprusside on antioxidant enzymes in wheat seedling roots under nickel stress

Wang, S. H.; Zhang, H.; Jiang, Sisi; Zhang, L.; He, Qun; He, Huan

doi:10.1134/s1021443710060129

Cited by 21 publications

(17 citation statements)

References 25 publications

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“…76 It has been reported that NO-induced GST participates in providing oxidative stress tolerance by reducing lipid peroxidation. 77 In the present study, GST (gi|15222163) is identified as putative S-nitrosylated target only after cold treatment [polypeptide 8, Figure S3 (Supporting Information) and Table 2] which indicated stressspecific S-nitrosylation in the apoplast. GPS algorithm of the identified GST predicted Cys 6 and Cys 20 as S-nitrosylation sites with a high threshold ( activity showed a two-fold increase with the cold treatment (p ≥ 0.019), while SNP (50 μM) treatment along with cold further increased the activity 2.9-fold (p ≥ 0.037, Figure 6C).…”

Section: Journal Of Proteome Researchmentioning

confidence: 62%

S-Nitrosylation Analysis in Brassica juncea Apoplast Highlights the Importance of Nitric Oxide in Cold-Stress Signaling

Sehrawat

Deswal

2014

J. Proteome Res.

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Reactive nitrogen species (RNS) including nitric oxide (NO) are important components of stress signaling. However, RNS-mediated signaling in the apoplast remains largely unknown. NO production measured in the shoot apoplast of Brassica juncea seedlings showed nonenzymatic nitrite reduction to NO. Thiol pool quantification showed cold-induced increase in the protein (including S-nitrosothiols) as well as non protein thiols. Proteins from the apoplast were resolved as 109 spots on the 2-D gel, while S-nitrosoglutathione-treated (a NO donor), neutravidin-agarose affinity chromatography-purified S-nitrosylated proteins were resolved as 52 spots. Functional categorization after MALDI-TOF/TOF identification showed 41 and 38% targets to be metabolic/cell-wall-modifying and stress-related, respectively, suggesting the potential role(s) of S-nitrosylation in regulating these responses. Additionally, identification of cold-stress-modulated putative S-nitrosylated proteins by nLC-MS/MS showed that only 38.4% targets with increased S-nitrosylation were secreted by classical pathway, while the majority (61.6%) of these were secreted by unknown/nonclassical pathways. Cold-stress-increased dehydroascorbate reductase and glutathione S-transferase activity via S-nitrosylation and promoted ROS detoxification by ascorbate regeneration and hydrogen peroxide detoxification. Taken together, cold-mediated NO production, thiol pool enrichment, and identification of the 48 putative S-nitrosylated proteins, including 25 novel targets, provided the preview of RNS-mediated cold-stress signaling in the apoplast.

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Section: Journal Of Proteome Researchmentioning

confidence: 62%

S-Nitrosylation Analysis in Brassica juncea Apoplast Highlights the Importance of Nitric Oxide in Cold-Stress Signaling

Sehrawat

Deswal

2014

J. Proteome Res.

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“…Despite this problematic rising of Ni in the environment, at low concentrations this metal is considered an essential micronutrient to plants, exerting an important role in several metabolic processes (e.g. N metabolism, and being a constituent of some enzymes, including glyoxalases, methyl coenzyme M reductase, acetyl coenzyme‐A synthase, as well as SOD and some hydrogenases; Wang et al ). However, in excess levels, Ni becomes strongly toxic to plants (Yusuf et al , Hussain et al , Soares et al ).…”

Section: No‐mediated Regulation Of Metal‐induced Oxidative Stressmentioning

confidence: 99%

Nitric oxide‐mediated regulation of oxidative stress in plants under metal stress: a review on molecular and biochemical aspects

Sharma

Soares

Sousa

et al. 2019

Physiologia Plantarum

118

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Given their sessile nature, plants continuously face unfavorable conditions throughout their life cycle, including water scarcity, extreme temperatures and soil pollution. Among all, metal(loid)s are one of the main classes of contaminants worldwide, posing a serious threat to plant growth and development. When in excess, metals which include both essential and non‐essential elements, quickly become phytotoxic, inducing the occurrence of oxidative stress. In this way, in order to ensure food production and safety, attempts to enhance plant tolerance to metal(loid)s are urgently needed. Nitric oxide (NO) is recognized as a signaling molecule, highly involved in multiple physiological events, like the response of plants to abiotic stress. Thus, substantial efforts have been made to assess NO potential in alleviating metal‐induced oxidative stress in plants. In this review, an updated overview of NO‐mediated protection against metal toxicity is provided. After carefully reviewing NO biosynthetic pathways, focus was given to the interaction between NO and the redox homeostasis followed by photosynthetic performance of plants under metal excess.

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“…10 μM SNP alleviates Al-induced inhibition of growth and impairment of the whole photosynthetic electron transport chain in sour pummelo (Citrus grandis) seedlings through increasing Al immobilization in roots and Al-induced secretion of malate and citrate from roots, and decreasing Al accumulation in shoots (Yang et al 2012). SNP, in a dose-dependent manner, significantly reverses the inhibitory effect of Ni on the growth of wheat (Triticum aestivum L.) seedlings through modulation of antioxidant enzymes (Wang et al 2010b). In particular, SNP enhances the activities of guaiacol peroxidase (POD, 0-48 h 10 µM SNP 24 h before HMs SNP prevents the inhibitory effect of HMs on root growth and morphology by the stimulation of SOD activity and ROS scavenging Kopyra and Gwóźdź (2003) Lycopersicon esculentum EC 1.11.1.7), APX, SOD, glutathione reductase (GR, EC 1.6.4.2), and glutathione S-transferase (GST, EC 2.5.1.18) in wheat seedling roots under nickel stress, while no significant difference in the activity of CAT has been observed with SNP with or without Ni (Wang et al 2010b).…”

Section: Effects Of No In the Protection Against Hms Stressmentioning

confidence: 99%

“…SNP, in a dose-dependent manner, significantly reverses the inhibitory effect of Ni on the growth of wheat (Triticum aestivum L.) seedlings through modulation of antioxidant enzymes (Wang et al 2010b). In particular, SNP enhances the activities of guaiacol peroxidase (POD, 0-48 h 10 µM SNP 24 h before HMs SNP prevents the inhibitory effect of HMs on root growth and morphology by the stimulation of SOD activity and ROS scavenging Kopyra and Gwóźdź (2003) Lycopersicon esculentum EC 1.11.1.7), APX, SOD, glutathione reductase (GR, EC 1.6.4.2), and glutathione S-transferase (GST, EC 2.5.1.18) in wheat seedling roots under nickel stress, while no significant difference in the activity of CAT has been observed with SNP with or without Ni (Wang et al 2010b). Interestingly, 100 μM SNP alleviates the adverse effect of deficient and toxic levels of Zn 2+ in both wheat and bean (Phaseolus vulgaris) seedlings by adjusting the levels of total and free sulfhydryl groups and increasing the level of reduced glutathione and the activity of SOD (Abdel-Kader 2007).…”

Section: Effects Of No In the Protection Against Hms Stressmentioning

confidence: 99%

Role of Nitric Oxide in Heavy Metal Stress

Cerana

Malerba

2015

Nitric Oxide Action in Abiotic Stress Responses in Plants

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Nitric oxide (NO) is a diffusible gaseous molecule first identified in mammalian systems as the endothelium-derived relaxing factor, a potent endogenous vasodilatator. NO is also synthesized and released by plants, where it is involved in several physiological processes and in adaptative response against different stresses; hence, NO is considered to be a general signal molecule in plant and animal cells. In recent years, heavy metals (HMs), naturally present or added to the soil through diverse anthropogenic activities, have become a problem of agricultural and environmental significance. Although the mechanism by which HMs affect plants integrity is not fully understood, there is increasing evidence suggesting that an important component of the responses against HM stress is NO. This chapter presents an overview on the effects of HMs on endogenous NO content. In addition, the role of exogenous-applied NO in alleviating HM toxicity is summarized and discussed.

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Effects of the nitric oxide donor sodium nitroprusside on antioxidant enzymes in wheat seedling roots under nickel stress

Cited by 21 publications

References 25 publications

S-Nitrosylation Analysis in Brassica juncea Apoplast Highlights the Importance of Nitric Oxide in Cold-Stress Signaling

S-Nitrosylation Analysis in Brassica juncea Apoplast Highlights the Importance of Nitric Oxide in Cold-Stress Signaling

Nitric oxide‐mediated regulation of oxidative stress in plants under metal stress: a review on molecular and biochemical aspects

Role of Nitric Oxide in Heavy Metal Stress

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