Effects of the marine toxin palytoxin on human skin keratinocytes: Role of ionic imbalance

Pelin, Marco; Zanette, Caterina; Bortoli, Marco; Sosa, Silvio; Loggia, R. Della; Tubaro, Aurelia; Florio, Chiara

doi:10.1016/j.tox.2011.01.010

Cited by 35 publications

(38 citation statements)

References 33 publications

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“…On HaCaT cells, removal of Na + from the cell medium almost completely abolished: i) PLTX-induced oxidative stress, ii) impairment of mitochondrial activity and iii) appearance of morphological changes, demonstrating that intracellular Na + accumulation is the first and crucial step in mediating PLTX-induced early cell damage. By contrast, Ca 2+ withdrawal did not affect PLTX-induced oxidative stress or cell morphology, confirming the Na + -dependency of these effects on HaCaT keratinocytes [48].…”

Section: Fig 2 | Structure Of Palytoxin (Pltx) Ostreocin-d (Ost-d)supporting

confidence: 61%

“…The high toxicity of PLTX on skin keratinocytes [48] raises valid concerns about the potential human exposure to PLTX-related toxins in seawater and needs to be further investigated. These data will help the prevention of toxicity for people exposed to seawater during Ostreopsis blooms, either professionally or recreationally.…”

Section: Discussionmentioning

confidence: 99%

“…These data suggest that the sequence of intracellular events following the interaction of PLTX with its molecular target includes mitochondrial damage, causing a reduction in cell viability and plasma membrane rupture, with resulting leakage of LDH. Moreover, mitochondrial dysfunction was tentatively associated with oxidative stress, since PLTX induced superoxide anion accumulation after only 1 h exposure [48]. All these effects were inhibited by the presence of ouabain, a cardiac glycoside that inhibits PLTX binding to its molecular target (Na + /K + -ATPase).…”

Section: Fig 2 | Structure Of Palytoxin (Pltx) Ostreocin-d (Ost-d)mentioning

confidence: 98%

“…Despite the reports on human dermal toxicity attributed to PLTXs, very little data regarding skin toxicity are presently available in the scientific literature. Our group contributed to the elucidation of the cutaneous effects of the parent compound PLTX: an in vitro toxicity study was carried out using the human HaCaT keratinocytes [48], a predictive model for the evaluation of skin toxicity and an ideal model for first-round screening of dermotoxic agents [49]. The cytotoxicity of PLTX on HaCaT cells was investigated after a short time exposure (4 h) and different cellular endpoints were evaluated.…”

Section: Fig 2 | Structure Of Palytoxin (Pltx) Ostreocin-d (Ost-d)mentioning

confidence: 99%

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Sanitary problems related to the presence of Ostreopsis spp. in the Mediterranean Sea: a multidisciplinary scientific approach

Favero

Sosa

Pelin

et al. 2012

Annali Dell'Istituto Superiore Di Sanità

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Abstract. The increased presence of potentially toxic microalgae in the Mediterranean area is a matter of great concern. Since the end of the last century, microalgae of the genus Ostreopsis have been detected more and more frequently in the Italian coastal waters. The presence of Ostreopsis spp. has been accompanied by the presence of previously undetected marine biotoxins (palytoxins) into the ecosystem with the increased possibility of human exposure. In response to the urgent need for toxicity characterization of palytoxin and its congeners, an integrated study encompassing both in vitro and in vivo methods was performed.

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Section: Fig 2 | Structure Of Palytoxin (Pltx) Ostreocin-d (Ost-d)supporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Fig 2 | Structure Of Palytoxin (Pltx) Ostreocin-d (Ost-d)mentioning

confidence: 98%

Section: Fig 2 | Structure Of Palytoxin (Pltx) Ostreocin-d (Ost-d)mentioning

confidence: 99%

See 2 more Smart Citations

Sanitary problems related to the presence of Ostreopsis spp. in the Mediterranean Sea: a multidisciplinary scientific approach

Favero

Sosa

Pelin

et al. 2012

Annali Dell'Istituto Superiore Di Sanità

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“…More specifically, an increase in sodium may stimulate calcium-independent superoxide anions and oxidative stress, leading to cellular death [9]. The toxicity can be severe affecting multiple organ systems and takes place after a short-time of exposure at very low concentration.…”

Section: Discussionmentioning

confidence: 99%

Palytoxin poisoning via inhalation in pediatric siblings

Rumore

Houst²

2014

IJCRI

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International Journal of Case Reports and Images (IJCRI) is an international, peer reviewed, monthly, open access, online journal, publishing high-quality, articles in all areas of basic medical sciences and clinical specialties.Aim of IJCRI is to encourage the publication of new information by providing a platform for reporting of unique, unusual and rare cases which enhance understanding of disease process, its diagnosis, management and clinico-pathologic correlations. Case Report: We report a case of palytoxin inhalational toxicity in pediatric siblings following secondary exposure to vapors from cleaning of an aquarium containing Zoanthids. Symptoms included fever, tachycardia, leukocytosis and elevated lactic dehydrogenase. Both patients received supportive treatment in the pediatric intensive care unit and were discharged after 48 hours. Symptoms also occurred in children's parents including cleaning attendant. Conclusion: Herein, we present a rare case of inhalational toxicity from palytoxin.

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