Effects of the kinase inhibitor CGP41251 (PKC 412) on lymphocyte activation and TNF-α production

Protein Kinase C as a Therapeutic Target

“…The N-benzoylated staurosporine analogue, midostaurin, was originally identified as a PKC inhibitor but has subsequently been found to inhibit both tyrosine kinase and serine/threonine kinases (57). Midostaurin is a potent radiation sensitizer both in cell culture and human tumor xenograft studies through a phosphoinositide-3-kinase/Akt pathway (58).…”

Effects of JAK3 Inhibition with CP-690,550 on Immune Cell Populations and Their Functions in Nonhuman Primate Recipients of Kidney Allografts

“…Our laboratory has previously developed pharmacodynamic (PD) assays incorporating flow cytometric analyses that focus on end products of specific intracellular biochemical cascades in lymphocytes, including cell-surface markers of lymphocyte activation, cytokine production by T-cells and lymphocyte proliferation. The relevancy of these assays in the estimation of the resulting effects of candidate immunosuppressive drugs has been established in small (17)(18)(19) and large (20 -22) animal models and was recently verified in human blood (20,(23)(24)(25) and in stable human recipients of kidney allografts (26).…”

cAMP/PKA Signaling Pathway Induces Apoptosis by Inhibited NF-κB in Aluminum Chloride-Treated Lymphocytes In Vitro

“…NF-κB, as a transcription factor, exists on TNF-α signal transduction pathway and cAMP/PKA signaling pathway in lymphocytes. And inhibiting NF-κB expressions can decrease TNF-α content [32][33][34][35]. Our previous research revealed that TNF-α contents decreased in AlCl 3 -treated lymphocytes of rats [21].…”