Effects of the introduction of Agrobacterium rhizogenes rol genes on tomato plant and flower development

Altvorst, A.C. van; Bino, R.J.; Dijk, A.J. van; Lamers, A.M.J.; Lindhout, W.H.; Mark, F. van der; Dons, J.J.M.

doi:10.1016/0168-9452(92)90064-s

Cited by 76 publications

(39 citation statements)

References 27 publications

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“…This gene was chosen because transgenic plants expressing it manifest several syndromes characteristic of auxin treatment (Schmulling et al 1988), though parthenocarpic fruit development was not reported in transgenic tomatoes expressing rolB under the control of its native promoter (van Altvorst et al 1992). Following our preliminary data indicating that, indeed, this transgene induces parthenocarpic fruit development in tomato Barg and Salts 2000), here we report a detailed analysis of the rolB-based parthenocarpy.…”

Section: Introductionmentioning

confidence: 97%

Induction of parthenocarpy in tomato via specific expression of the rolB gene in the ovary

Carmi

Salts

Dedičová

et al. 2003

Planta

113

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The molecular signals for the development of the ovary into fruit following ovule fertilization are not clear. However, in many species, including tomato ( Lycopersicon esculentum Mill.), auxins and auxin transport inhibitors can substitute for fertilization as activators of fruit set, suggesting that this plant hormone plays a key role in this process. In agreement, transgenes for auxin biosynthesis expressed under ovary- or ovule-specific promoters were shown earlier to enable parthenocarpic (i.e. seedless) fruit development. In the present study, we tested an alternative approach for the induction of parthenocarpy that is based on ovary-specific expression of the Agrobacterium rhizogenes-derived gene rolB. This gene was chosen because rolB transgenic plants manifest several syndromes characteristic of auxin treatment. Tomato plants transformed with a chimeric construct containing the rolB gene fused to the ovary- and young-fruit-specific promoter TPRP-F1 developed parthenocarpic fruits. Fruit size and morphology, including jelly fill in the locules of the seedless fruits, were comparable to those of seeded fruits of the parental line. Although it is not known whether ROLB signals for the same cassette of genes involved in fertilization-dependent fruit development, it clearly activates a battery of genes that enable successful completion of seedless fruit development in tomato.

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Section: Introductionmentioning

confidence: 97%

Induction of parthenocarpy in tomato via specific expression of the rolB gene in the ovary

Carmi

Salts

Dedičová

et al. 2003

Planta

113

View full text Add to dashboard Cite

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“…Constitutive rolB expression suppressed the growth of tobacco (Nicotiana tabacum) cells, and the rolC gene was able to attenuate this growth inhibition (Schmü lling et al, 1988). Likewise, rolC diminished the rolB-induced high sensitivity to auxin in transformed cells (Maurel et al, 1991) and the severity of rolB-induced phenotypes (Capone et al, 1989;Vanaltvorst et al, 1992). Recently, a contrasting difference between the action of rolB and rolC on class III peroxidase gene expression has been demonstrated (Veremeichik et al, 2012).…”

Section: Combined Effect Of the Rolb And Rolc Genesmentioning

confidence: 99%

TherolBGene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

et al. 2012

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The rolB (for rooting locus of Agrobacterium rhizogenes) oncogene has previously been identified as a key player in the formation of hairy roots during the plant-A. rhizogenes interaction. In this study, using single-cell assays based on confocal microscopy, we demonstrated reduced levels of reactive oxygen species (ROS) in rolB-expressing Rubia cordifolia, Panax ginseng, and Arabidopsis (Arabidopsis thaliana) cells. The expression of rolB was sufficient to inhibit excessive elevations of ROS induced by paraquat, menadione, and light stress and prevent cell death induced by chronic oxidative stress. In rolB-expressing cells, we detected the enhanced expression of antioxidant genes encoding cytosolic ascorbate peroxidase, catalase, and superoxide dismutase. We conclude that, similar to pathogenic determinants in other pathogenic bacteria, rolB suppresses ROS and plays a role not only in cell differentiation but also in ROS metabolism.

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“…Although the rolB and rolC genes are often referred to as closely related genes, they demonstrated differing or even opposite effects not only on the process of cell death (Schmülling et al 1988), but also calcium balance in transformed cells (Bulgakov et al 2003), auxin sensitivity (Maurel et al 1991), growth of transformed tissues (Capone et al 1989;van Altvorst et al 1992) and secondary metabolism . This list could be expanded upon when considering the different effects of rolB and rolC on the expression of class III peroxidase genes.…”

Section: Discussionmentioning

confidence: 98%

Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

et al. 2012

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Here, seven new class III peroxidase genes of Rubia cordifolia L., RcPrx01-RcPrx07, were isolated and characterized. Expression of the Prx genes was studied in R. cordifolia aerial organs as well as in cells transformed with the rolB and rolC genes of Agrobacterium rhizogenes and cells transformed with the wild-type A. rhizogenes A4 strain. In rolC- and rolB-transformed cells, the rol genes were expressed under the control of the 35S promoter, whereas in A. rhizogenes A4-transformed cells the rol genes were expressed under the control of their native promoters. All studied peroxidase genes were greatly upregulated in rolB-overexpressing cells. In contrast, overexpression of the rolC gene and expression of the rol genes under the control of their native promoters had little effect on the abundance of peroxidase transcripts. In accordance with this observation, peroxidase activity was substantially increased in rolB cells and was slightly affected in other transformed cells. Our results indicate that rolB strictly affects the regulation of a set of seven R. cordifolia class III peroxidases.

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Effects of the introduction of Agrobacterium rhizogenes rol genes on tomato plant and flower development

Cited by 76 publications

References 27 publications

Induction of parthenocarpy in tomato via specific expression of the rolB gene in the ovary

Induction of parthenocarpy in tomato via specific expression of the rolB gene in the ovary

TherolBGene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

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