Effects of the intradiscal implantation of stromal vascular fraction plus platelet rich plasma in patients with degenerative disc disease

Abstract

BackgroundStromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. The SVF contains a mixture of cells including ADSCs and growth factors and has been depleted of the adipocyte (fat cell) population. We evaluated the safety and efficacy of administering SVF and PRP intra-discally into patients with degenerative disc disease.MethodsA total of 15 patients underwent a local tumescent liposuction procedure to remove approximately 60 ml of fat tissue. The fat was separated to isolate the SVF and the cells were delivered into the disc nucleus of patients with degenerative disc disease. The subjects were then monitored for adverse events, range of motion, visual analog scale (VAS), present pain intensity (PPI), Oswestry Disability Index (ODI), Beck Depression Inventory (BDI), Dallas Pain Questionnaire and Short Form (SF)-12 scores over a period of 6 months. Safety events were followed for 12 months.ResultsNo severe adverse events (SAEs) were reported during a 12 month follow up period with no incidences of infection. Patients demonstrated statistically significant improvements in several parameters including flexion, pain ratings, VAS, PPI, and short form questionnaires. In addition, both ODI and BDI data was trending positive and a majority of patients reported improvements in their Dallas Pain Questionnaire scores.ConclusionsOverall, patients were pleased with the treatment results. More importantly, the procedure demonstrated a strong safety profile with no severe adverse events or complications linked to the therapy. Trial registration NCT02097862. Name of registry: www.clinicaltrials.gov. https://clinicaltrials.gov/ct2/show/NCT02097862?term=bioheart&rank=6. Date of registration: March 25, 2014; Date of enrollment: March 2014

Short-term hypoxic preconditioning promotes prevascularization in 3D bioprinted bone constructs with stromal vascular fraction derived cells

“…39,40 The mesodermal cell population, defined as SVF, is typically harvested by enzymatic digestion and centrifugation, and contains a heterogeneous array of cell types, including mesenchymal stem cells, endothelial cells, perivascular cells, fibroblasts, and immune cells. 41,42 SVF has gained increased popularity and has wide applicability in the regeneration of the spinal cord, 43 intervertebral disc, 44 and cartilage. 45,46 However, most SVF were directly injected into the target tissues or generated into pellets for implantation without in vitro expansion.…”

Regenerative Medicine for Axial and Radicular Spine‐Related Pain: A Narrative Review

“…Regarding prospective trials, the results were positive for PRP, with pain outcomes improving in the majority of PRP‐treated patients . Notably, the Comella et al study used a combination of stromal vascular fraction (SVF) and PRP for their injectate; SVF is a combination of ADSCs and growth factors.…”

“…Regarding prospective trials, the results were positive for PRP, with pain outcomes improving in the majority of PRP-treated patients. 41,42 Notably, the Comella et al study used a combination of stromal vascular fraction (SVF) and PRP for their injectate; SVF is a combination of ADSCs and growth factors. The remaining prospective PRP study found 47% of patients had a greater than 50% improvement in VAS scores and a 30% decrease in Oswestry Disability Index (ODI) scores at 6 months.…”

Micro Fragmented Adipose Tissue Promotes the Matrix Synthesis Function of Nucleus Pulposus Cells and Regenerates Degenerated Intervertebral Disc in a Pig Model

“…The SVF has already been used in Achilles tendinopathy treatment, breast reconstruction, and other treatments in clinical trials 25,26 . Comella et al treated patients with IVD degeneration by intradiscal implantation of SVF plus platelet-rich plasma, and a majority of patients reported improvements in several parameters, including flexion and pain rating 27 . However, the isolation of the SVF by traditional methods such as collagenase does not bode well with regulatory authorities 28 .…”