Effects of the interaction and surface morphology of mixed DPoPE/Poloxamer 188 monolayers and thin liquid films

Jordanova, A.; Tenchov, Boris; Lalchev, Zdravko

doi:10.1039/c1sm05165b

Cited by 6 publications

(2 citation statements)

References 48 publications

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“…14,15,33 Langmuir surface balance experiments Surface pressure (p)-area (A) isotherms were measured using a computer-controlled Langmuir surface balance (Kibron, Helsinki, Finland) equipped with a mTrough XL (area 225 cm 2 , volume 40 ml) by the Wilhelmy wire probe method. 34,35 Human meibum dissolved in chloroform was spread (43 ml of 1 mg ml À1 ) over the air/saline solution interface with a Hamilton microsyringe. An acrylic cover was put over the trough to protect from dust contamination and to suppress subphase evaporation.…”

Section: Collection Of Lipid Samplesmentioning

confidence: 99%

Surface chemistry study of the interactions of hyaluronic acid and benzalkonium chloride with meibomian and corneal cell lipids

et al. 2013

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Hyaluronic acid (HA) is a non-surface active water soluble anionic polyelectrolyte found in sub-nanomolar levels in human tears and implemented in substitutes of human tears' aqueous layer. In the current work we studied the interactions of high molecular weight (M w 1 Â 10 6 Da) HA with films of ocular surface lipids and investigated the potential of HA to modify the interactions of the lipid films with the low M w cationic surfactant dodecyldimethylbenzylammonium chloride (C12-BAC) known for its adverse effects on tear film compounds. The interactions of HA and/or C12-BAC with meibomian and corneal lipid films were examined using a Langmuir surface balance. The film morphology was monitored by Brewster Angle Microscopy. The HA/C12-BAC interactions at the air/water interface and in the solution bulk were also examined. HA interacted with meibomian lipids and enhanced the structure and the rheological properties of the meibum films. HA bound C12-BAC in bulky polymer/surfactant complexes and at $0.1% HA neutralized the capability of C12-BAC to penetrate the lipid layers and to disrupt their integrity and viscoelasticity. The results were correlated with the capability of HA to maintain the viability of C12-BAC treated corneal cells. Our study suggests a possible novel implementation of high M w anionic polyelectrolytes like HA: the polymers can be instilled on the ocular surface together with cationic surfactant containing drugs, and increase the biocompatibility of the ophthalmic formulations.A molecular scale mechanism is proposed for the interactions of HA with tear lipids and cationic surfactants.

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Section: Collection Of Lipid Samplesmentioning

confidence: 99%

Surface chemistry study of the interactions of hyaluronic acid and benzalkonium chloride with meibomian and corneal cell lipids

et al. 2013

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“…A detailed description of the cell and the experimental procedure is presented in ref. [33][34][35][36][37].…”

Section: Experimental Materialsmentioning

confidence: 99%

Foam film study of albumin inhibited lung surfactant preparations: effect of added hydrophilic polymers

et al. 2012

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The effect of polyethylene glycol-modified lipids on the interaction of HIV-1 derived peptide–dendrimer complexes with lipid membranes

Melikishvili

Poturnayová

Йонов

et al. 2016

Biochimica et Biophysica Acta (BBA) - Biomembranes

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In this study, dendrimers have been purposed as an alternative approach for delivery of HIV peptides to dendritic cells. We have investigated the interaction of dendriplexes formed from polyanionic HIV peptide Nef and cationic carbosilane dendrimer (CBD) with model lipid membranes - large unilamellar vesicles (LUVs), Langmuir monolayers and supported lipid membranes (sBLMs) containing various molar ratio of zwitterionic 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG). In our experiments, the lipid membranes represented the model of the plasma membrane of the cell. PEGylated lipids were used in order to model glycocalyx which constitutes the outer leaflet of cellular membranes. The presence of PEGylated lipids resulted in an increase of the phase transition temperature of the lipid bilayer of LUVs, in a decrease of specific volume and adiabatic compressibility. Fluorescence anisotropy study suggests that PEGylated LUVs possessed higher lipid order and decreased fluidity when compared to zwitterionic DMPC vesicles. The interaction of dendriplexes with monolayers was accompanied by the formation of the aggregates as revealed by BAM experiments. This conclusion has been confirmed also by AFM imaging of sBLMs. We have demonstrated that dendriplexes interact with lipid membranes for all types of lipid composition. Moreover, the stronger interaction of cationic dendrimer/peptide complexes with lipid monolayers, vesicles and sBLMs was observed for membranes composed of zwitterionic lipids than for PEGylated lipid membranes. Increased concentration of PEGylated lipids made this interaction weaker.

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Effects of the interaction and surface morphology of mixed DPoPE/Poloxamer 188 monolayers and thin liquid films

Cited by 6 publications

References 48 publications

Surface chemistry study of the interactions of hyaluronic acid and benzalkonium chloride with meibomian and corneal cell lipids

Surface chemistry study of the interactions of hyaluronic acid and benzalkonium chloride with meibomian and corneal cell lipids

Foam film study of albumin inhibited lung surfactant preparations: effect of added hydrophilic polymers

The effect of polyethylene glycol-modified lipids on the interaction of HIV-1 derived peptide–dendrimer complexes with lipid membranes

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