Effects of the insecticide fipronil in freshwater model organisms and microbial and periphyton communities

Pino-Otín, María Rosa; Ballestero, Diego; Navarro, Enrique; Mainar, Ana M.; Val, Jonatan

doi:10.1016/j.scitotenv.2020.142820

Cited by 16 publications

(6 citation statements)

References 128 publications

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“…Pesticides including insecticides are foreseeable tools of agronomic practices used to protect the crops from damaging effect of various pests. − After application, these chemicals reach the soils and negatively interact with soil microorganisms . The irregular and indiscriminate use of such chemical pesticides leads to the destruction of physicochemical processes of soil, microbial structure, physiology, and enzymatic activity . Soil microorganisms are an important biological component of the soil ecosystem and have a vital role in the fertility of soil through decomposition of organic matter and nutrient cycling .…”

Section: Introductionmentioning

confidence: 99%

Organochlorine Pesticides Negatively Influenced the Cellular Growth, Morphostructure, Cell Viability, and Biofilm-Formation and Phosphate-Solubilization Activities of Enterobacter cloacae Strain EAM 35

et al. 2021

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An in vitro study was conducted to assess the impact of organochlorine pesticides (OCPs) on cellular growth, morphology, cell viability, biofilm-formation activity, and growthregulating substances of a soil bacterium. Phosphate-solubilizing EAM 35 isolated from rhizosphere soil was molecularly identified as Enterobacter cloacae (accession number MT672578.1). Strain EAM 35 tolerated varying levels of OCPs, viz., benzene hexachloride (BHC), chlorpyrifos (CP), dieldrin (DE), and endosulfan (ES). The toxicity of OCPs to strain EAM 35 was displayed in a concentration-dependent manner. Among the OCPs, ES at a concentration of 200 μM showed a higher toxicity, where it maximally reduced the bacterial synthesis of indole-3-acetic acid (IAA), salicylic acid (SA), and 2,3-dihydroxy-benzoic acid (DHBA) by 73% (p ≤ 0.001), 85% (p ≤ 0.005), and 83% (p ≤ 0.001), respectively, over the control. While comparing the toxicity of OCPs to P-solubilizing activity of E. cloacae after 10 days of growth, the toxicity pattern followed the order ES (mean value = 82.6 μg mL −1 ) > CP (mean value = 93.2 μg mL −1 ) > DE (mean value = 113.6 μg mL −1 ) > BHC (mean value = 127 μg mL −1 ). Furthermore, OCP-induced surface morphological distortion in E. cloacae EAM 35 was observed as gaps, pits on both cellular facets, and fragmented and disorganized cell structure under a scanning electron microscope (SEM). The membrane-compromised cells increased as the concentrations of OC pesticides increased from 25 to 200 μM. Additionally, microbial counts (log 10 CFU/mL) were also affected after pesticide exposure and decreased with increasing concentrations. While assessing the impact of OCPs on inhibition (%) of log 10 CFU/mL, 150, 175, and 200 μM concentrations of ES completely reduced the growth of E. cloacae. Similarly, while comparing the toxicity of higher concentrations of OCPs to bacterial growth, sensitivity followed the order ES > DE > CP > BHC. In addition, the biofilm-formation ability of strain EAM 35 was inhibited in a pesticide-dose-dependent manner, and it was statistically (p ≤ 0.05, p ≤ 0.005, and p ≤ 0.001) significant. Conclusively, the present study clearly suggests that before applying pesticides to soil, their recommended dose should carefully be monitored.

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Section: Introductionmentioning

confidence: 99%

Organochlorine Pesticides Negatively Influenced the Cellular Growth, Morphostructure, Cell Viability, and Biofilm-Formation and Phosphate-Solubilization Activities of Enterobacter cloacae Strain EAM 35

et al. 2021

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“…As mentioned above, the fipronil contamination of soils can alter the diversity of microbial communities [ 9 , 35 , 36 ]. This is because the high toxicity of fipronil and the relatively low water activity found in soils probably act as a selective pressure for the growth of microorganisms; in other words, only those microorganisms possessing the enzymatic machinery that enable them to degrade these compounds are able to survive.…”

Section: Resultsmentioning

confidence: 99%

“…In this context, in the recent study by Park et al [ 8 ], fipronil was found to be toxic to zebrafish ( Danio rerio ) embryos at 2.5 mg/L and above. In other studies, with environmentally relevant concentrations, lethality was observed for the microcrustacean Daphnia magna from 0.07 to 0.3 mg/L, and inhibition of the photosynthetic activity of the chlorophycean microalga Chlamydomonas reinhardtii at 2.4 mg/L [ 9 ]. Biochemical and genetic response modifications were also observed in organisms exposed to fipronil.…”

Section: Introductionmentioning

confidence: 99%

Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective

Prado,

Pereira,

Durrant

et al. 2023

Life

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Fipronil is an insecticide widely used in the agricultural and veterinary sectors for its efficacy in pest control. The presence of fipronil in the environment is mainly due to agricultural and domestic practices and is frequently found in different types of environmental matrices in concentrations ranging from µg/L to mg/L and can be hazardous to non-target organisms due to its high toxicity. This study was carried out to obtain and characterize microorganisms from soil which are capable of biodegrading fipronil that could be of great biotechnological interest. For this purpose, bioprospecting was carried out using fipronil (0.6 g/L) as the main source of carbon and nitrogen for growth. Once obtained, the strain was identified by sequencing the 16S ribosomal RNA (rRNA) gene and the capacity to degrade fipronil was monitored by GC-MS. Our study showed a presence in soil samples of the strain identified as Enterobacter chengduensis, which was able to metabolize fipronil and its metabolites during the mineralization process. Enterobacter chengduensis was able to biodegrade fipronil (96%) and its metabolites fipronil-sulfone (92%) and fipronil-sulfide (79%) in 14 days. Overall, the results of this study provided a bacterium with great potential that could contribute to the degradation of fipronil in the environment.

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“…However, evaluating the toxicity of the commercial formula, we present more relevant data for a realistic risk assessment of fipronil-based insecticides to non-target freshwater animals nearby agricultural areas. Although fipronil has low to moderate water solubility, due to its widespread use, the insecticide is currently present in soils and surface and groundwater, and direct or indirect effects on aquatic organisms can be expected [49]. Thus, we speculate the potential deleterious effects of the commercial compound Regent 800 WG ® on tropical freshwater planarians, considering that in an aquatic environment, without the presence of light (places inhabited by planarians), the half-life of fipronil may be relatively long-with a half-life ranging between 36 h and 7.3 months depending on substrate and conditions [50].…”

Section: Discussionmentioning

confidence: 99%

Responses of Freshwater Planarian Girardia tigrina to Fipronil-Based Insecticide: Survival, Behavioral and Physiological Endpoints

et al. 2022

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Fipronil is a pyrazole insecticide used to control undesirable insect populations. Due to its large-scale application, there is the potential for surface waters’ contamination, with toxic action for non-target organisms, and consequent impacts on aquatic ecosystems. Planarians are potential non-target aquatic invertebrates to these insecticides. They are widespread in tropical freshwaters and have been proposed as good candidates to assess the toxic effects of freshwater systems contaminated by insecticides. Thus, the present study aims to evaluate the sublethal concentrations of a fipronil-based insecticide that may affect the planarian physiology. After chronic exposure to Regent 800 WG®, a significant decrease in locomotor velocity (LOEC—6.25 mg·L−1), regeneration of the auricles and photoreceptors (LOEC—3.13 mg·L−1), and reproduction (fecundity—LOEC 12.5 mg·L−1) were observed. The results of our study demonstrate that long-term exposure to a pyrazole insecticide can compromise non-target aquatic invertebrates while reinforcing the need for a better investigation of complementary parameters (such as behavior, regeneration, and reproduction) for a more accurate risk assessment of commercial pesticide toxicity in freshwater systems.

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Effects of the insecticide fipronil in freshwater model organisms and microbial and periphyton communities

Cited by 16 publications

References 128 publications

Organochlorine Pesticides Negatively Influenced the Cellular Growth, Morphostructure, Cell Viability, and Biofilm-Formation and Phosphate-Solubilization Activities of Enterobacter cloacae Strain EAM 35

Organochlorine Pesticides Negatively Influenced the Cellular Growth, Morphostructure, Cell Viability, and Biofilm-Formation and Phosphate-Solubilization Activities of Enterobacter cloacae Strain EAM 35

Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective

Responses of Freshwater Planarian Girardia tigrina to Fipronil-Based Insecticide: Survival, Behavioral and Physiological Endpoints

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