Effects of the inhibitor of the γ-aminobutyrate-transaminase, vinyl-γ-aminobutyrate, on development and nitrogen metabolism in Brassica napus seedlings

Deleu, Carole; Faes, Pascal; Niogret, Marie‐Françoise; Bouchereau, Alain

doi:10.1016/j.plaphy.2012.12.007

Cited by 24 publications

(13 citation statements)

References 52 publications

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“…Thus, VGB might have a specific role in inhibiting early primary root growth. However, the GABA treatment did not adversely affect the roots, which was consistent with the findings of a previous study involving Brassica napus seedlings [60], but it inhibited chestnut seed germination. Furthermore, soluble protein contents reportedly decreased during the seed germination of six grass species [61], but they increased significantly when the primary root growth was inhibited at 120 h after both treatments in the current study ( Figure 5).…”

Section: Discussionsupporting

confidence: 91%

“…Therefore, the observed decrease in most of the amino acid levels at the 48 h time-point following the GABA and VGB treatments might contribute to the inhibition of chestnut seed germination (Figure 6). At 120 h, however, only the VGB treatment induced a considerable increase in the accumulation of 10 amino acids ( Figure 6), which were reportedly negatively associated with root growth [13,37,[58][59][60]. Thus, VGB might have a specific role in inhibiting early primary root growth.…”

Section: Discussionmentioning

confidence: 97%

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Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Chen

et al. 2020

Plants

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Recalcitrant chestnut seeds are rich in γ-aminobutyric acid (GABA), which negatively regulates adventitious root development by altering carbon/nitrogen metabolism. However, little is known regarding the role of this metabolite in chestnut seeds. In this study, we investigated the effects of GABA changes on the germination of chestnut seeds treated with exogenous GABA and vigabatrin (VGB, which inhibits GABA degradation). Both treatments significantly inhibited seed germination and primary root growth and resulted in the considerable accumulation of H2O2, but the endogenous GABA content decreased before germination at 48 h. Soluble sugar levels increased before germination, but subsequently decreased, whereas starch contents were relatively unchanged. Changes to organic acids were observed at 120 h after sowing, including a decrease and increase in citrate and malate levels, respectively. Similarly, soluble protein contents increased at 120 h, but the abundance of most free amino acids decreased at 48 h. Moreover, the total amino acid levels increased only in response to VGB at 0 h. Accordingly, GABA and VGB altered the balance of carbon and nitrogen metabolism, thereby inhibiting chestnut seed germination. These results suggested that changes to GABA levels in chestnut seeds might prevent seed germination. The study data may also help clarify the dormancy and storage of chestnut seeds, as well as other recalcitrant seeds.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 97%

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Chen

et al. 2020

Plants

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“…Metabolite analysis was performed on a Waters Acquity ultraperformance liquid chromatography machine with diode array detection (UPLC-DAD) using methods and software described in the Waters Corporation user manual. The manual was adapted for oilseed rape tissue by [ 34 , 35 ]. The AccQtag method was used to quantify amino acids and the integration software Empower (Waters Corporation, Milford, USA) was used for analysis.…”

Section: Methodsmentioning

confidence: 99%

Drought stress has transgenerational effects on seeds and seedlings in winter oilseed rape (Brassica napus L.)

Nuppenau²,

et al. 2018

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BackgroundDrought stress has a negative effect on both seed yield and seed quality in Brassica napus (oilseed rape, canola). Here we show that while drought impairs the maternal plant performance, it also increases the vigour of progeny of stressed maternal plants. We investigated the transgenerational influence of abiotic stress by detailed analysis of yield, seed quality, and seedling performance on a growth-related and metabolic level. Seeds of eight diverse winter oilseed rape genotypes were generated under well-watered and drought stress conditions under controlled-environment conditions in large plant containers.ResultsWe found a decrease in seed quality in seeds derived from mother plants that were exposed to drought stress. At the same time, the seeds that developed under stress conditions showed higher seedling vigour compared to non-stressed controls.This effect on seed quality and seedling vigour was found to be independent of maternal plant yield performance.ConclusionsDrought stress has a positive transgenerational effect on seedling vigour. Three potential causes for stress-induced improvement of seedling vigour are discussed: (1) Heterotic effects caused by a tendency towards a higher outcrossing rate in response to stress; (2) an altered reservoir of seed storage metabolites to which the seedling resorts during early growth, and (3) inter-generational stress memory, formed by stress-induced changes in the epigenome of the seedling.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1531-y) contains supplementary material, which is available to authorized users.

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“…Analysis was performed on a Waters Acquity ultra‐performance liquid chromatography with diode array detection using methods and software described in the Waters Corporation user manual and developed for oilseed rape tissue by Albert et al () and Deleu et al (). The AccQtag Method was used to quantify aminoacids and integration software Empower (Waters Corporation, Milford, USA) was used for analysis.…”

Section: Methodsmentioning

confidence: 99%

Early Osmotic Adjustment Responses in Drought‐Resistant and Drought‐Sensitive Oilseed Rape

Hatzig

Zaharia

Abrams

et al. 2014

JIPB

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The impact of osmotic stress on growth, physiology, and metabolism of winter oilseed rape (Brassica napus L.) was investigated by detailed analysis of biomass traits, hormone metabolites and osmolytes in two genetically unrelated drought-tolerant genotypes and two unrelated drought-sensitive genotypes. Seedlings were grown in vitro under controlled conditions and osmotic stress was simulated by applying a gradual treatment with polyethylene glycol (PEG 6000), followed by hypo-osmotic treatment of variants used for metabolite determination. The results provide a basis for the identification of reliable selection criteria for drought resistance in oilseed rape. The in vitro cultivation system established during this study enabled effective discrimination of early osmotic stress responses between drought-resistant and -susceptible oilseed rape genotypes that also show large differences in relative seed yield under drought conditions in the field. Clear physiological and metabolic differences were observed between the drought-resistant and drought-sensitive genotypes, suggesting that osmotic adjustment is a key component of drought response in oilseed rape. Unexpectedly, however, the drought-resistant genotypes did not show typical hormonal adjustment and osmolyte accumulation, suggesting that they possess alternative physiological mechanisms enabling avoidance of stress symptoms.

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Effects of the inhibitor of the γ-aminobutyrate-transaminase, vinyl-γ-aminobutyrate, on development and nitrogen metabolism in Brassica napus seedlings

Cited by 24 publications

References 52 publications

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Drought stress has transgenerational effects on seeds and seedlings in winter oilseed rape (Brassica napus L.)

Early Osmotic Adjustment Responses in Drought‐Resistant and Drought‐Sensitive Oilseed Rape

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