Effects of the in ovo injection of vitamin D3 and 25-hydroxyvitamin D3 in Ross 708 broilers subsequently fed commercial or calcium and phosphorus-restricted diets. I. Performance, carcass characteristics, and incidence of woody breast myopathy1,2,3

Fatemi, Seyed Abolghasem; Alqhtani, Abdulmohsen H.; Elliott, K.E.C.; Bello, A.; Zhang, H.; Peebles, E. D.

doi:10.1016/j.psj.2021.101220

Cited by 13 publications

(25 citation statements)

References 49 publications

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“…The improvement in small intestine morphology observed in response to the in ovo injection of 25OHD 3 alone, may be due to the longer half-life and higher rate of absorption of 25OHD 3 . Previous findings in our laboratory revealed that breast meat yield increased ( Fatemi et al, 2021 a , b) and FCR decreased ( Fatemi et al, 2020a ) in response to the in ovo injection of 2.4 μg of 25OHD 3 . An improvement in small intestine morphology and humoral immunity is associated with increased breast meat yield and an improvement in the growth performance of broilers ( Chou et al, 2009 ; Wang et al, 2019 ).…”

Section: Discussionmentioning

confidence: 70%

“…All experimental procedures were approved by the Institutional Animal Care and Use Committee of Mississippi State University (Protocol #IACUC-17-406). Fertile broiler hatching eggs laid by 35 wk-old Ross 708 breeder hens were obtained from a commercial source and stored for 24 h. Prior to set, eggs were selected as described by Sokale et al (2017) and a total of 2,880 eggs (average weight = 59.8 ± 0.81) were subsequently set and incubated in a Jamesway model PS 500 setter unit (Jamesway Incubator Company Inc., Cambridge, Ontario, Canada) under standard conditions (37.5° C and 29.4° C dry and wet bulb temperatures, respectively) as is described by Fatemi et al (2021b) . Thirty eggs was assigned to each of 4 preassigned treatment groups on each of 24 incubator tray levels (blocks).…”

Section: Methodsmentioning

confidence: 99%

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Effects of the in ovo injection of vitamin D3 and 25-hydroxyvitamin D3 in Ross 708 broilers subsequently fed commercial or calcium and phosphorus-restricted diets. II. Immunity and small intestine morphology1,2,3

Fatemi

Elliott²,

Bello³

et al. 2021

Poultry Science

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Effects of the in ovo injection of vitamin D 3 ( D 3 ) and 25-hydroxyvitamin D 3 ( 25OHD 3 ) on the immunity and small intestine morphology of broilers fed calcium and phosphorus-restricted diets were investigated. At 18 d of incubation ( doi ), live embryonated Ross 708 broiler hatching eggs were in ovo-injected with a 50 μL solution of one of the following treatments using an Inovoject multiegg injector: 1) diluent (control); diluent containing either 2) 2.4 μg D 3 ; 3) 2.4 μg 25OHD 3 ; or 4) 2.4 μg D 3 + 2.4 μg 25OHD 3 . At hatch, 18 randomly selected male broilers belonging to one of the 4 in ovo injection treatments were placed in each of 12 floor pens and were fed either a commercial diet or a diet restricted by 20% in calcium and available phosphorus ( ReCaP ) content for the starter, grower and finisher dietary phases. Concentrations of plasma IgG and IgM at 14 d of age ( doa ) and α-1-acid glycoprotein at 40 doa were determined. Bursa, liver, spleen, duodenum, jejunum, and ileum weights were recorded at 7, 14, and 40 doa and small intestine histology was evaluated at 14 and 40 doa. Blood and organ samples were randomly collected from 1 bird in each of the 6 replicate pens within each of the 8 (4 in ovo x 2 dietary) treatment groups. Plasma IgG levels were higher in 25OHD 3 than in diluent or D 3 in ovo - injected birds. At 14 doa, a higher jejunal villus length ( VL ) to crypt depth ( CD ) ratio ( RVC ) was observed in birds that were in ovo - injected with 25OHD 3 alone as compared to all other in ovo injection treatments. At 40 doa, ileal VL increased and jejunal CD decreased in commercial diet-fed birds compared to ReCaP diet-fed birds. In conclusion, the in ovo injection of 25OHD 3 alone increased the immune response and improved the small intestine morphology and subsequent nutrient uptake of Ross 708 broilers. However, a ReCaP diet was observed to be detrimental to their small intestine morphology.

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Section: Discussionmentioning

confidence: 70%

Section: Methodsmentioning

confidence: 99%

Effects of the in ovo injection of vitamin D3 and 25-hydroxyvitamin D3 in Ross 708 broilers subsequently fed commercial or calcium and phosphorus-restricted diets. II. Immunity and small intestine morphology1,2,3

Fatemi

Elliott²,

Bello³

et al. 2021

Poultry Science

Self Cite

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“…Furthermore, dietary 25OHD 3 has been reported to increase the BWG of broilers challenged with coccidiosis ( Morris et al., 2014 ; Leyva-Jimenez et al., 2019 ). In addition, when compared to the in ovo injection of D 3 or diluent alone, the in ovo injection of 2.4 of μg of 25OHD 3 has been shown to increase the breast meat yield ( Fatemi et al., 2021a , b ) and improve the live performance ( Fatemi et al., 2021a ) of Ross 708 broilers. This same treatment has also been shown to comparatively improve small intestine morphology ( Fatemi et al., 2021c ) and immunity ( Fatemi et al., 2021a , c ) of the broilers.…”

Section: Discussionmentioning

confidence: 99%

Effects of the in ovo injection of vitamin D3 and 25-hydroxyvitamin D3 in Ross 708 broilers subsequently challenged with coccidiosis. I. performance, meat yield and intestinal lesion incidence1,2,3

et al. 2021

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Effects of the in ovo administration of vitamin D 3 ( D 3 ) and 25-hydroxyvitamin D 3 ( 25OHD 3 ) on broiler intestinal lesion incidence, performance and breast meat yield after a coccidiosis challenge were investigated. On each of 10 incubator tray levels, 10 Ross 708 broiler hatching eggs were randomly assigned to each of the following 5 in ovo injection treatments administrated at 18 d of incubation ( doi ): 1) noninjected; 2) diluent; diluent containing either 3) 2.4 μg D 3 (D 3 ), 4) 2.4 μg 25OHD 3 (25OHD 3 ), or 5) 2.4 μg D 3 + 2.4 μg 25OHD 3 (D 3 +25OHD 3 ). A 50 μL solution volume was injected into each egg using an Inovoject multi-egg injector. Four male chicks were randomly assigned to each of 80 battery cages in each of 2 rooms. Half of the treatment-replicate cages (8) in each room were challenged with a 20× live coccidial vaccine at 14 d of age ( doa ). One randomly selected bird from each of 4 treatment-replicate cages was scored for coccidiosis lesions before and 2 wk after challenge. Mean BW, BW gain ( BWG) , feed intake, and feed conversion ratio were determined for all birds from 0 to 14, 15 to 28, and 29 to 41 doa. Carcass weight, and the absolute and relative (% of carcass weight) weights of carcass parts were determined in 3 birds per treatment-replicate cage at 42 doa. Hatchability of live embryonated injected eggs and hatch residue were not affected by treatment. Across challenge treatment, birds in the 25OHD 3 treatment group experienced an increase in BWG between 29 and 41 doa when compared to the D 3 or diluent-injected birds. Furthermore, pectoralis major muscle percentage tended ( P = 0.059) to increase in birds belonging to the 25OHD 3 treatment in comparison to birds in the D 3 or diluent-injected treatments. These results indicate that regardless of challenge treatment, 2.4 μg of 25OHD 3 may increase the BWG and breast meat yield of birds relative to those that only received an injection of commercial diluent.

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“…All in ovo injection solutions were prepared according to the procedures described by Zhang et al [ 17 ]. After injection, eggs were transferred to the hatchling baskets according to the method described by Fatemi et al [ 18 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, in ovo injection has been used to successfully deliver different nutrients such as vitamins, minerals, carbohydrates, and amino acids to broiler embryos [ 16 ]. In ovo injection of vitamins has shown promising results for broiler hatchability [ 16 , 17 , 18 ] post hatch performance [ 2 , 16 , 17 , 19 ], breast meat yield [ 19 , 20 ], meat quality [ 2 , 16 ], immunity [ 16 , 19 ], and antioxidant activity [ 2 , 16 ]. Zhang et al [ 16 ] and Mousstaaid et al [ 21 ] reported that the in ovo injection of 100 μL of L-AA in a dosage range from 0.5 to 25 mg/mL was safe and resulted in no negative effects on hatchability and hatchling quality.…”

Section: Introductionmentioning

confidence: 99%

Effects of the In Ovo and Dietary Supplementation of L-Ascorbic Acid on the Growth Performance, Inflammatory Response, and Eye L-Ascorbic Acid Concentrations in Ross 708 Broiler Chickens

Mousstaaid

Fatemi

Elliott

et al. 2022

Animals

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Effects of the dietary and in ovo administration of L-ascorbic acid (L-AA) on the performance, plasma nitric oxide, and eye L-AA concentrations of Ross 708 broilers were investigated. At 17 days of incubation, live embryonated hatching eggs were randomly assigned to a non-injected or sham-injected (100 μL of saline) control group, or a group injected with either 12 or 25 mg of L-AA suspended in 100 μL of saline. Chicks received a commercial diet with or without 200 mg/kg of supplemental L-AA and were randomly assigned to each of 6 replicate floor pens in each in ovo injection-dietary treatment combination. Weekly live performance variables through 14 days of post hatch age (doa) and the eye weights in both sexes at 0, 7, and 14 doa were determined. At 0 and 14 doa, plasma nitric oxide levels and eye L-AA concentrations of one bird of each sex in each pen were determined. Dietary supplemental L-AA decreased feed intake and growth between 0 and 7 doa, but from 8 to 14 doa; all birds fed supplemental L-AA had a lower feed conversion ratio. At 14 doa, male chicks had higher eye L-AA concentrations and lower plasma nitric oxide levels when treated in ovo with 12 mg of L-AA. In conclusion, dietary L-AA may be used to improve feed conversion in the second week of broiler post hatch growth. However, the in ovo administration of 12 mg of L-AA can increase male eye L-AA concentrations and is effective in reducing their general inflammatory response.

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Effects of the in ovo injection of vitamin D3 and 25-hydroxyvitamin D3 in Ross 708 broilers subsequently fed commercial or calcium and phosphorus-restricted diets. I. Performance, carcass characteristics, and incidence of woody breast myopathy1,2,3

Cited by 13 publications

References 49 publications

Effects of the in ovo injection of vitamin D3 and 25-hydroxyvitamin D3 in Ross 708 broilers subsequently fed commercial or calcium and phosphorus-restricted diets. II. Immunity and small intestine morphology1,2,3

Effects of the in ovo injection of vitamin D3 and 25-hydroxyvitamin D3 in Ross 708 broilers subsequently fed commercial or calcium and phosphorus-restricted diets. II. Immunity and small intestine morphology1,2,3

Effects of the in ovo injection of vitamin D3 and 25-hydroxyvitamin D3 in Ross 708 broilers subsequently challenged with coccidiosis. I. performance, meat yield and intestinal lesion incidence1,2,3

Effects of the In Ovo and Dietary Supplementation of L-Ascorbic Acid on the Growth Performance, Inflammatory Response, and Eye L-Ascorbic Acid Concentrations in Ross 708 Broiler Chickens

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