Effects of the <i>FABP4</i> gene on steroid hormone secretion in goose ovarian granulosa cells

Fan, Ruiming; Cao, Zhenzhen; Chen, M; Wang, H; Liu, M; Gao, Ming; L, Xin

doi:10.1080/00071668.2020.1817325

Cited by 6 publications

(2 citation statements)

References 51 publications

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“…In granulosa cells, a series of enzymes are responsible for converting cholesterol into steroid hormones. Thus, emzymes such as P450scc (encoded by CYP11A1 ) catalyzes the first rate-limiting step in the conversion of cholesterol to pregnenolone, whereas cytochrome P450 aromatase (coded by CYP19A1 ) is involved in the last step of estrogen biosynthesis [ 41 , 42 ]. In this study, it was revealed that miR-30a-5p promoted the synthesis of steroid hormones in the granulosa cells.…”

Section: Discussionmentioning

confidence: 99%

miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

Tian

et al. 2022

J Animal Sci Biotechnol

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Background The egg production performance of chickens is affected by many factors, including genetics, nutrition and environmental conditions. These factors all play a role in egg production by affecting the development of follicles. MicroRNAs (miRNAs) are important non-coding RNAs that regulate biological processes by targeting genes or other non-coding RNAs after transcription. In the animal reproduction process, miRNA is known to affect the development and atresia of follicles by regulating apoptosis and autophagy of granulosa cells (GCs). Results In this study, we identified potential miRNAs in the atretic follicles of broody chickens and unatretic follicles of healthy chickens. We identified gga-miR-30a-5p in 50 differentially expressed miRNAs and found that gga-miR-30a-5p played a regulatory role in the development of chicken follicles. The function of miR-30a-5p was explored through the transfection test of miR-30a-5p inhibitor and miR-30a-5p mimics. In the study, we used qPCR, western blot and flow cytometry to detect granulosa cell apoptosis, autophagy and steroid hormone synthesis. Confocal microscopy and transmission electron microscopy are used for the observation of autophagolysosomes. The levels of estradiol (E2), progesterone (P4), malondialdehyde (MDA) and superoxide dismutase (SOD) were detected by ELISA. The results showed that miR-30a-5p showed a negative effect on autophagy and apoptosis of granulosa cells, and also contributed in steroid hormones and reactive oxygen species (ROS) production. In addition, the results obtained from the biosynthesis and dual luciferase experiments showed that Beclin1 was the target gene of miR-30a-5p. The rescue experiment conducted further confirmed that Beclin1 belongs to the miR-30a-5p regulatory pathway. Conclusions In summary, after deep miRNA sequencing on healthy and atretic follicles, the results indicated that miR-30a-5p inhibits granulosa cell death by inhibiting Beclin1.

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Section: Discussionmentioning

confidence: 99%

miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

Tian

et al. 2022

J Animal Sci Biotechnol

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“…Gpx3 codes for glutathione peroxidase, where glutathione protects cells from oxidative stress and is associated with fertility 31 . Notably, among genes that shared a similar expression pattern as Edn2, we found an enrichment of genes regulating cell metabolism, including Akr1c18 32 (more commonly known as 20alpha-HSD; metabolizes progesterone to its inactive form), Rora (an orphan nuclear receptor regulating cell differentiation, circadian gene expression and cell metabolism), Ldhd (lactate dehydrogenase D; catabolizes D-lactate to pyruvate), and Fabp4 33 (regulates long-chain fatty transport and metabolism), which is consistent with the results from gene ontology enrichment analysis (Supp. Fig.…”

Section: Spatiotemporal Response Of Mural Granulosa Cells To Ovulatio...mentioning

confidence: 97%

A Spatiotemporal Molecular Atlas of the Ovulating Mouse Ovary

Mantri,

Zhang,

Spanos

et al. 2023

Preprint

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Ovulation is essential for reproductive success, yet the underlying cellular and molecular mechanisms are far from clear. Here, we applied high-resolution spatiotemporal transcriptomics to map out cell-type- and ovulation-stage-specific molecular programs as function of time during follicle maturation and ovulation in mice. Our analysis revealed dynamic molecular transitions within granulosa cell types that occur in tight coordination with mesenchymal cell proliferation. We identified new molecular markers for the emerging cumulus cell fate during the preantral-to-antral transition. We describe transcriptional programs that respond rapidly to ovulation stimulation and those associated with follicle rupture, highlighting the prominent roles of apoptotic and metabolic pathways during the final stages of follicle maturation. We further report stage-specific oocyte-cumulus cell interactions and diverging molecular differentiation in follicles approaching ovulation. Collectively, this study provides insights into the cellular and molecular processes that regulate mouse ovarian follicle maturation and ovulation with important implications for advancing therapeutic strategies in reproductive medicine.

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Comparative liver transcriptome analysis of duck reveals potential genes associated with egg production

et al. 2022

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Effects of the FABP4 gene on steroid hormone secretion in goose ovarian granulosa cells

Cited by 6 publications

References 51 publications

miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

A Spatiotemporal Molecular Atlas of the Ovulating Mouse Ovary

Comparative liver transcriptome analysis of duck reveals potential genes associated with egg production

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