Effects of the husky oil spill on gut microbiota of native fishes in the North Saskatchewan River, Canada

DeBofsky, Abigail; Xie, Yuwei; Jardine, Timothy D.; Hill, Janet E.; Jones, Paul D.; Giesy, John P.

doi:10.1016/j.aquatox.2020.105658

Cited by 18 publications

(6 citation statements)

References 43 publications

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“…Such a result does not seem to reflect any protective effect of the parasite toward PAHs, but is rather due to the lower Shannon index recorded in infected chub. The decrease in alpha diversity indexes is consistent with previous studies showing that gut microbial diversity of freshwater fish was inversely correlated with PAHs levels in the muscles of wild fish exposed to an oil spill 43 or with the dietary exposure level of juveniles to benzo[a]pyrene 44 . Similar observations were made in marine systems, where exposure to crude oil or PAH-contaminated sediments was associated with a loss of gut microbial diversity 3,45 .…”

Section: Discussionsupporting

confidence: 91%

“…This lack of microbial response to this contaminant was unexpected, given that PAHs and their metabolites were detected in the liver and muscle and that individuals experienced liver hypertrophy 38 . It was also not in line with a number of studies that evidenced an effect of PAHs, sometimes even characterized by the recruitment of hydrocarbonoclastic bacteria 3,43,44,[48][49][50] . This discrepancy may stem from the fact that the PAH concentration tested in this work is much lower than those generally applied in mesocosm studies or recorded during acute pollution episodes.…”

Section: Discussioncontrasting

confidence: 58%

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Dysbiosis of fish gut microbiota is associated with helminths parasitism rather than exposure to PAHs at environmentally relevant concentrations

Colin

Molbert

Berthe

et al. 2022

Sci Rep

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Although parasite infection and pollution are common threats facing wild populations, the response of the gut microbiota to the joint impact of these stressors remains largely understudied. Here, we experimentally investigated the effects of exposure to Polycyclic Aromatic Hydrocarbons (PAHs) and infection by a common acanthocephalan intestinal parasite (Pomphorhynchus sp.) on the gut microbial flora of a freshwater fish, the European chub (Squalius cephalus). Naturally infected or uninfected individuals were exposed to PAHs at environmentally realistic concentrations over a five-week period. Characterization of the gut bacterial community through 16S rRNA gene amplicon sequencing revealed that parasitic infection was a more structuring factor of bacterial diversity and composition than PAH exposure. Specifically, chub infected by Pomphorhynchus sp. harbored significantly less evenly represented gut bacterial communities than the uninfected ones. In addition, substantial changes in sequence abundance were observed within the main bacterial phyla, including the Firmicutes, Fusobacteriota, Actinobacteriota, and Proteobacteria. Again, these compositional changes correlated with host infection with Pomphorhynchus sp., confirming its pivotal role in gut microbial assemblage. Overall, these results highlight the importance of defining the parasitic status of individuals when conducting microbial ecotoxicological analyses at the digestive tract level, as this should lead to better understanding of microbiota modulations and help to identify microbial markers specifically associated with chemicals.

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Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 58%

Dysbiosis of fish gut microbiota is associated with helminths parasitism rather than exposure to PAHs at environmentally relevant concentrations

Colin

Molbert

Berthe

et al. 2022

Sci Rep

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“…The negative control test group showed that the normal microbiome of L. calcarifer on a commercial fishmeal diet contains core taxa of phyla Proteobacteria (62%), Firmicutes (7%), Planctomycetes (3%), Actinobacteria (2%), and Bacteroidetes (1%). This is typical and agrees with other microbiome studies on L. calcarifer (Gupta et al, 2019;Zheng et al, 2019;Chaklader et al, 2021a) and other species (Ghanbari et al, 2015;Adamovsky et al, 2018;DeBofsky et al, 2020).…”

Section: At the Phylum Levelsupporting

confidence: 92%

“…Exposure to anthropogenic toxicants such as metals and petroleum hydrocarbons also alters the gut microbiome in fish, as illustrated by field studies following the Deepwater Horizon (DWH) spill ( Brown-Peterson et al, 2015 ) and a riverine oil spill in Saskatchewan, Canada ( DeBofsky et al, 2020 ). Laboratory studies have shown that red bream ( Pagrus major ) exposed to phenanthrene produces significant changes in gut microbiome ( Hano et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Gut Microbiome as a Potential Biomarker in Fish: Dietary Exposure to Petroleum Hydrocarbons and Metals, Metabolic Functions and Cytokine Expression in Juvenile Lates calcarifer

et al. 2022

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The gut microbiome of fish contains core taxa whose relative abundances are modulated in response to diet, environmental factors, and exposure to toxicogenic chemicals, influencing the health of the host fish. Recent advances in genomics and metabolomics have suggested the potential of microbiome analysis as a biomarker for exposure to toxicogenic compounds. In this 35-day laboratory study, 16S RNA sequencing and multivariate analysis were used to explore changes in the gut microbiome of juvenile Lates calcarifer exposed to dietary sub-lethal doses of three metals: vanadium (20 mg/kg), nickel (480 mg/kg), and iron (470 mg/kg), and to two oils: bunker C heavy fuel oil (HFO) (1% w/w) and Montara, a typical Australian medium crude oil (ACO) (1% w/w). Diversity of the gut microbiome was significantly reduced compared to negative controls in fish exposed to metals, but not petroleum hydrocarbons. The core taxa in the microbiome of negative control fish comprised phyla Proteobacteria (62%), Firmicutes (7%), Planctomycetes (3%), Actinobacteria (2%), Bacteroidetes (1%), and others (25%). Differences in the relative abundances of bacterial phyla of metal-exposed fish were pronounced, with the microbiome of Ni-, V-, and Fe-exposed fish dominated by Proteobacteria (81%), Firmicutes (68%), and Bacteroidetes (48%), respectively. The genus Photobacterium was enriched proportionally to the concentration of polycyclic aromatic hydrocarbons (PAHs) in oil-exposed fish. The probiotic lactic acid bacterium Lactobacillus was significantly reduced in the microbiota of fish exposed to metals. Transcription of cytokines IL-1, IL-10, and TNF-a was significantly upregulated in fish exposed to metals but unchanged in oil-exposed fish compared to negative controls. However, IL-7 was significantly downregulated in fish exposed to V, Ni, Fe, and HFOs. Fish gut microbiome exhibits distinctive changes in response to specific toxicants and shows potential for use as biomarkers of exposure to V, Ni, Fe, and to PAHs present in crude oil.

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“…Previous studies have shown that water level fluctuation has a significant influence on the migration of organic pollutants throughout the water table [ 21 ], suggesting that the dissolution and biodegradation of LNAPL components is likely to increase [ 22 ]. Due to the potentially widespread and dispersed nature of petroleum hydrocarbon plumes, the remediation process can be lengthy, requiring cost-effective and practical methods to ensure the long-term control of contaminated groundwater [ 23 ]. Compared to other remediation techniques, natural attenuation (NA) has been recognized as one of the remediation options that can meet the aforementioned requirements [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

The Migration Mechanism of BTEX in Single- and Double-Lithology Soil Columns under Groundwater Table Fluctuation

Zheng,

Yang,

et al. 2023

Toxics

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The migration of light non-aqueous phase liquids (LNAPLs) trapped in porous media is a complex phenomenon. Groundwater table fluctuation can not only affect contaminant migration but also redox conditions, bacterial communities, and contaminant degradation. Understanding LNAPLs’ (e.g., benzene, toluene, ethylbenzene, and xylene (BTEX)) behavior within porous media is critical for the high efficiency of most in situ remediation systems. A laboratory study of single- and double-lithology soil column investigation of the groundwater table fluctuation effect on BTEX transport, using benzene and toluene as typical compounds, in a typical representative model of aquifers subjected to water table fluctuation was undertaken in this study. The results show that benzene and toluene migration in single-lithology soil columns packed with sand was mainly affected by flushing due to the hydraulic force induced by water table fluctuations and that the double-lithology soil column packed with sand and silt was significantly affected by retention due to the higher adsorption induced by 10 cm of silt. The dissolution mainly correlated with the BTEX migration in saturated zones, and the contaminant concentration increased when the water table fell and decreased when the water table rose. For a contaminated site with a single-lithology structure consisting of sand, more attention should be paid to organic contaminant removal within the groundwater, and a double-lithology structure containing silt is more suited to the removal of organic contaminants from the silt layer. The difference in biodegradation kinetics between the groundwater table fluctuation (GTF) zone and the saturated zone should be better understood for the remediation of BTEX compounds.

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Effects of the husky oil spill on gut microbiota of native fishes in the North Saskatchewan River, Canada

Cited by 18 publications

References 43 publications

Dysbiosis of fish gut microbiota is associated with helminths parasitism rather than exposure to PAHs at environmentally relevant concentrations

Dysbiosis of fish gut microbiota is associated with helminths parasitism rather than exposure to PAHs at environmentally relevant concentrations

Gut Microbiome as a Potential Biomarker in Fish: Dietary Exposure to Petroleum Hydrocarbons and Metals, Metabolic Functions and Cytokine Expression in Juvenile Lates calcarifer

The Migration Mechanism of BTEX in Single- and Double-Lithology Soil Columns under Groundwater Table Fluctuation

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