Effects of the halophilic fungi Cladosporium sphaerospermum, Wallemia sebi, Aureobasidium pullulans and Aspergillus nidulans on halite formed on sandstone surface

Mansour, M. H.

doi:10.1016/j.ibiod.2017.01.016

Cited by 11 publications

(5 citation statements)

References 58 publications

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“…In both, glucose is the starting compound for these synthetic processes. Cell wall thickening by increased β-1,3-glucan and synthesis of compatible solutes are two of the main adaptations to halophily reported for fungi, specifically in the genus Aspergillus : A. niger ( Diano et al, 2006 ), A. nidulans ( Mansour, 2017 ), A. oryzae ( Ruijter et al, 2004 ), A. sydowii ( Pérez-Llano et al, 2020 ; Rodríguez-Pupo et al, 2021 ) and also reported in this work. This shift to non-lipid sources may also be related to the maintenance of appropriate cytoplasmic membrane fluidity, an important factor for the indispensable functioning of transporter families that are activated in response to osmotic changes in halophilic and halotolerant fungi ( Turk et al, 2004 ).…”

Section: Discussionsupporting

confidence: 80%

Surviving in the Brine: A Multi-Omics Approach for Understanding the Physiology of the Halophile Fungus Aspergillus sydowii at Saturated NaCl Concentration

et al. 2022

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Although various studies have investigated osmoadaptations of halophilic fungi to saline conditions, only few analyzed the fungal mechanisms occurring at saturated NaCl concentrations. Halophilic Aspergillus sydowii is a model organism for the study of molecular adaptations of filamentous fungi to hyperosmolarity. For the first time a multi-omics approach (i.e., transcriptomics and metabolomics) was used to compare A. sydowii at saturated concentration (5.13 M NaCl) to optimal salinity (1 M NaCl). Analysis revealed 1,842 genes differentially expressed of which 704 were overexpressed. Most differentially expressed genes were involved in metabolism and signal transduction. A gene ontology multi-scale network showed that ATP binding constituted the main network node with direct interactions to phosphorelay signal transduction, polysaccharide metabolism, and transferase activity. Free amino acids significantly decreased and amino acid metabolism was reprogrammed at 5.13 M NaCl. mRNA transcriptional analysis revealed upregulation of genes involved in methionine and cysteine biosynthesis at extreme water deprivation by NaCl. No modifications of membrane fatty acid composition occurred. Upregulated genes were involved in high-osmolarity glycerol signal transduction pathways, biosynthesis of β-1,3-glucans, and cross-membrane ion transporters. Downregulated genes were related to the synthesis of chitin, mannose, cell wall proteins, starvation, pheromone synthesis, and cell cycle. Non-coding RNAs represented the 20% of the total transcripts with 7% classified as long non-coding RNAs (lncRNAs). The 42% and 69% of the total lncRNAs and RNAs encoding transcription factors, respectively, were differentially expressed. A network analysis showed that differentially expressed lncRNAs and RNAs coding transcriptional factors were mainly related to the regulation of metabolic processes, protein phosphorylation, protein kinase activity, and plasma membrane composition. Metabolomic analyses revealed more complex and unknown metabolites at saturated NaCl concentration than at optimal salinity. This study is the first attempt to unravel the molecular ecology of an ascomycetous fungus at extreme water deprivation by NaCl (5.13 M). This work also represents a pioneer study to investigate the importance of lncRNAs and transcriptional factors in the transcriptomic response to high NaCl stress in halophilic fungi.

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Section: Discussionsupporting

confidence: 80%

Surviving in the Brine: A Multi-Omics Approach for Understanding the Physiology of the Halophile Fungus Aspergillus sydowii at Saturated NaCl Concentration

et al. 2022

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“…Aureobasidium pullulans is a ubiquitous species of black yeast, saprophytic in plants and frequent in the air of urban environments [40], but also found on monument surfaces [41][42][43]. Dothidea berberidis is included in the order Dothideales, comprising melanized fungi from rocks [44].…”

Section: Organisms On the Cross Of The Inquisition Limestonementioning

confidence: 99%

“…Sci. 2022, 12, 6222 10 of 16 [41][42][43]. Dothidea berberidis is included in the order Dothideales, comprising melanized fungi from rocks [44].…”

Section: Organisms On the Cross Of The Inquisition Limestonementioning

confidence: 99%

Holistic Approach to the Restoration of a Vandalized Monument: The Cross of the Inquisition, Seville City Hall, Spain

et al. 2022

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The Cross of the Inquisition, sculpted in 1903 and raised on a column with a fluted shaft and ornamented with vegetable garlands, is located in a corner of the Plateresque façade of the Seville City Hall. The Cross was vandalized in September 2019 and the restoration concluded in September 2021. A geological and microbiological study was carried out in a few small fragments. The data are consistent with the exposure of the Cross of the Inquisition to an urban environment for more than 100 years. During that time, a lichen community colonized the Cross and the nearby City Hall façades. The lichens, bryophytes and fungi colonizing the limestone surface composed an urban community, regenerated reorganized from the remains of the original communities, after superficial cleaning of the limestone between 2008 and 2010. This biological activity was detrimental to the integrity of the limestone, as showed by the pitting and channels, which evidence the lytic activity of organisms on the stone surface. Stone consolidation was achieved with Estel 1000. Preventol RI80, a biocide able to penetrate the porous limestone and active against bacteria, fungi, lichens, and bryophytes, was applied in the restoration.

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“…Halophilic fungi grew in high salt concentrations, some may be extreme halophiles (adapted to high salt concentration), there are mandatory halophilic fungi (need a high concentration of salts to grow) and facultative halophilic fungi (do not require high salt concentrations and tolerate up to 15‰ salinity). Different species of Cladosporium, Aspergillus, Penicillium and Wallemia spp are important representatives (Mansour, 2017).…”

Section: Introductionmentioning

confidence: 99%

Detoxification of BTEX in a saline environment by Aspergillus niger

Rodrigues,

Salgado,

Galhanas

et al. 2024

Preprint

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BTEX can be found in marine and estuarine waters due to accidental spills of oil and derivatives, as well as in production water and effluents from petrochemical plants. Solutions involving the bioremediation of these compounds in both saline environments and effluents with high salinity are necessary. The halotolerance of Aspergillus niger was tested by subjecting it to a gradual increase in salinity through the sequential addition of NaCl from 2 to 30‰ (v/v). It supported its growth up to 25‰, with a biomass production rate of 0.93 ± 0.11 g/d. The adapted biomass was used in batch reactor assays to test the biodegradation of BTEX (1,500 mg/L). The fungi removed BTEX more rapidly in the presence of 2 g/L of sucrose: benzene (2.20 d-1) > toluene (1.83 d-1) > ethylbenzene (1.12 d-1) > xylene (0.91 d-1). However, even in the absence of sucrose, there was removal in the reactors with fungi: benzene (1.13 d-1) > toluene (0.97 d-1) > ethylbenzene (0.77 d-1) > xylene (0.68 d-1), with higher removal through microbial action (absorption and adsorption). Benzene was the only compound found adsorbed to the mycelium (1.50 ± 0.19 to 13.35 ± 4.72 mg/g of biomass).

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Effects of the halophilic fungi Cladosporium sphaerospermum, Wallemia sebi, Aureobasidium pullulans and Aspergillus nidulans on halite formed on sandstone surface

Cited by 11 publications

References 58 publications

Surviving in the Brine: A Multi-Omics Approach for Understanding the Physiology of the Halophile Fungus Aspergillus sydowii at Saturated NaCl Concentration

Surviving in the Brine: A Multi-Omics Approach for Understanding the Physiology of the Halophile Fungus Aspergillus sydowii at Saturated NaCl Concentration

Holistic Approach to the Restoration of a Vandalized Monument: The Cross of the Inquisition, Seville City Hall, Spain

Detoxification of BTEX in a saline environment by Aspergillus niger

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