Effects of the gene carrier polyethyleneimines on structure and function of blood components

Zhong, Dagen; Jiao, Yanpeng; Zhang, Yi; Zhang, Wei; Li, Nan; Zuo, Qinhua; Wang, Qian; Wang, Xue; Liu, Zonghua

doi:10.1016/j.biomaterials.2012.09.060

Cited by 123 publications

(109 citation statements)

References 53 publications

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“…The advantages of PEI include high condensation capability toward DNA, strong buffering capacity in a pH range of 5.1-7.4, and relatively high transfection efficiency [8]. These characteristics make PEI the gold standard for newly designed non-viral vectors, especially among cationic polymers.…”

Section: Introductionmentioning

confidence: 99%

In vitro study of partially hydrolyzed poly(2-ethyl-2-oxazolines) as materials for biomedical applications

Shah

Kroneková

Zahoranová

et al. 2015

J Mater Sci: Mater Med

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Polymers based on 2-oxazoline, such as poly(2-ethyl-2-oxazolines) (PETOx), are considered to be a type of 'pseudopeptide' with the ability to form novel biomaterials. The hydrolysis of PETOx was carried out to evaluate its use in biomedical applications. In the present work, PETOx samples with a range of molar masses were prepared by living cationic polymerization. Hydrolysis was carried out at time intervals ranging from 15 to 180 min to prepare copolymers with different amounts of ethylene imine units. (1)H NMR spectroscopy was used to identify the structure of the hydrolyzed polymers. The dependence of in vitro cell viability on the degree of hydrolysis was determined using three different model cell lines, namely, mouse embryonic 3T3 fibroblasts, pancreatic βTC3 cells, and mouse lymphoid macrophages P388.D1. It was demonstrated that increasing the degree of hydrolysis decreased cell viability for all cell types. Fibroblast cells displayed the highest tolerance; additionally, the effect of polymer size showed no observable significance. Macrophage cells, immune system representatives, displayed the highest sensitivity to contact with hydrolyzed PETOx. The effect of polymer hydrolysis, polymer concentration and the incubation time on cell viability was experimentally observed. Confocal laser-scanning microscopy provided evidence of cellular uptake of pyrene-labeled (co)polymers.

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Section: Introductionmentioning

confidence: 99%

In vitro study of partially hydrolyzed poly(2-ethyl-2-oxazolines) as materials for biomedical applications

Shah

Kroneková

Zahoranová

et al. 2015

J Mater Sci: Mater Med

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“…the internalization of the cargo into cancer cells, and thus may alter the expected anti-tumor efficacy. Following administration, nano-carriers tend to bind/interact with various components that are present in biofluids [31], including proteins and enzymes forming the so called 'protein corona' [32,33]. For instance, recent reports suggest that the targeting capability of ligands conjugated to nanomaterials is lost by adsorption of a protein corona to their surface [34,35].…”

Section: Introductionmentioning

confidence: 99%

Colloidal stability of nano-sized particles in the peritoneal fluid: Towards optimizing drug delivery systems for intraperitoneal therapy

et al. 2014

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Intraperitoneal (IP) administration of nano-sized delivery vehicles containing small interfering RNA (siRNA) is recently gaining attention as an alternative route for the efficient treatment of peritoneal carcinomatosis. The colloidal stability of nanomatter following IP administration has, however, not been thoroughly investigated yet. Here, enabled by advanced microscopy methods such as Single Particle Tracking (SPT) and Fluorescence Correlation Spectroscopy (FCS), we follow the aggregation and cargo release of nano-scaled systems directly in peritoneal fluids from healthy mice and ascites fluid from a patient diagnosed with peritoneal carcinomatosis. The colloidal stability in the peritoneal fluids was systematically studied in function of the charge (positive or negative) and Poly-Ethylene Glycol (PEG) degree of liposomes and polystyrene nanoparticles, and compared to human serum. Our data demonstrate strong aggregation of cationic and anionic nanoparticles in the peritoneal fluids, while only slight aggregation was observed for the PEGylated ones. PEGylated liposomes, however, lead to a fast and premature release of siRNA cargo in the peritoneal fluids. Based on our observations, we reflect on how to tailor improved delivery systems for IP therapy.

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“…However, one of the major problems described for PEIs is that they can be toxic to certain cell types, including neurons, depending on MW, structure and concentration of the molecule used. In fact, high-MW PEIs cause necrotic-like changes due to alterations in the plasma membrane, followed by activation of a mitochondrial-mediated apoptosis in several cell types [27,28]. In contrast, low-MW PEIs displayed much less toxicity but almost no transfection efficiency.…”

Section: Synthetic Polymersmentioning

confidence: 99%

Nanoparticles for Brain-Specific Drug and Genetic Material delivery, Imaging and Diagnosis

Posadas

Monteagudo

Ceña

2016

Nanomedicine (Lond.)

100

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The poor access of therapeutic drugs and genetic material into the central nervous system due to the presence of the blood-brain barrier often limits the development of effective noninvasive treatments and diagnoses of neurological disorders. Moreover, the delivery of genetic material into neuronal cells remains a challenge because of the intrinsic difficulty in transfecting this cell type. Nanotechnology has arisen as a promising tool to provide solutions for this problem. This review will cover the different approaches that have been developed to deliver drugs and genetic material efficiently to the central nervous system as well as the main nanomaterials used to image the central nervous system and diagnose its disorders.

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Effects of the gene carrier polyethyleneimines on structure and function of blood components

Cited by 123 publications

References 53 publications

In vitro study of partially hydrolyzed poly(2-ethyl-2-oxazolines) as materials for biomedical applications

In vitro study of partially hydrolyzed poly(2-ethyl-2-oxazolines) as materials for biomedical applications

Colloidal stability of nano-sized particles in the peritoneal fluid: Towards optimizing drug delivery systems for intraperitoneal therapy

Nanoparticles for Brain-Specific Drug and Genetic Material delivery, Imaging and Diagnosis

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