Effects of the Garlic Components Diallyl Sulfide and Diallyl Disulfide on Arylamine N-Acetyltransferase Activity in Human Colon Tumour Cells

Chen, G. W.; Chung, Jing‐Gung; Hsieh, Ching-Liang; Lin, Jaung Gung

doi:10.1016/s0278-6915(98)00046-5

Cited by 47 publications

(13 citation statements)

References 31 publications

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“…The slow and fast acetylator phenotypes have been associated with increased risk for cancers of the bladder and colon, respectively. DAS and DADS decreased the activity of this enzyme in strains of H. pylori from peptic ulcer patients (31) and inhibited its activity in a human colon tumor cell line (91) and in human bladder tumor cells (92) in a dose-dependent manner.…”

Section: Reviews • Allium Vegetables and Cancer Preventionmentioning

confidence: 95%

Allium vegetables and organosulfur compounds: do they help prevent cancer?

Bianchini

Vainio

2001

Environ Health Perspect

192

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The Allium genus includes approximately 500 species, the most widely used of which are onions (Allium cepa), garlic (Allium sativum), leeks (Allium porrum), chives (Allium schoenoprasum), and shallots (Allium ascalonicum). Garlic is widely cultivated and consumed worldwide, and its beneficial effects have been known for thousands of years. It has been considered to increase longevity and to confer stamina and physical strength, and it has been used empirically as a vermifuge, antiseptic, antimicrobic, antipyretic, and analgesic. The scientific community has now become interested in the pharmacologic properties of Allium vegetables and their chemical constituents, particularly with regard to their effects on the cardiovascular system and in the prevention of cancer. Garlic has antihypertensive and antiarrythmic properties and exerts an antithrombotic effect through fibrinolytic activity and the reduction of platelet aggregation. Ingestion of garlic has also been reported to lower the concentration of triglycerides, cholesterol, and low-density lipoproteins and to increase the concentration of high-density lipoproteins in blood. These findings suggest that garlic has a preventive effect against atherosclerosis and its complications, including stroke, myocardial infarction, and thrombotic disorders. The findings in relation to cancer prevention that have been accumulated over the last 20 years are summarized here.We performed a systematic Medline search of the published literature on epidemiologic studies involving the relationship between consumption of Allium vegetables-garlic, onions, and leeks-and neoplasms/tumors. For the animal studies and for studies on mechanisms of action, we conducted searches for review articles. These lists of references were then updated using references from review papers and original research articles. Active Compounds in Allium VegetablesFresh garlic contains water, carbohydrates, proteins, fiber, and fat, as well as essential amino acids, vitamins, and minerals. When garlic is cut, chopped, or crushed, the clove's membrane is disrupted and S-allylcysteine sulfoxide (an odorless compound called alliine) is transformed enzymatically into allicin by alliinase (1). Allicin is responsible for the typical odor of garlic, but it is unstable and converts readily into mono-, di-, and trisulfides and other compounds such as ajoene. Onions mainly contain S-propenylcysteine sulfoxide (1), but also other sulfoxides, including S-propylcysteine sulfoxide and S-methylcysteine sulfoxide (2). S-Propenylcysteine sulfoxide, positional isomer of alliin, is called lacrimatory precursor, because it is tranformed by alliinase into the lacrimatory factor propanethial S-oxide. The lacrimatory factor is highly reactive and hydrolyzes to propionaldehyde, sulfuric acid, and hydrogen sulfide; it is also the precursor of several sulfur derivatives. Organosulfur compounds present in Allium vegetables, which are either lipid or water soluble (Table 1), are considered responsible for the beneficial effects of these...

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Section: Reviews • Allium Vegetables and Cancer Preventionmentioning

confidence: 95%

Allium vegetables and organosulfur compounds: do they help prevent cancer?

Bianchini

Vainio

2001

Environ Health Perspect

192

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“…Caffeic acid, esculetin, quercetin, kaemferol and genistein inhibited NAT1 whereas scopuletin and coumarin inhibited NAT2 88 . Chen et al 89 and Lin et al 90 described the effect of diallyl sulfide (DAS) and diallyl disulfide (DADS), major components of garlic, on NAT activity in human colon tumor cells and human promyelocytic leukemia cells. These studies demonstrated that DAS and DADS markedly inhibited NAT activity in these cells and would thus assist the organism in defense against cancer.…”

Section: Substrates Inhibitors and Inductors Of Natsmentioning

confidence: 99%

Phase Ii Drug Metabolizing Enzymes

Jančová¹,

Anzenbacher²,

Anzenbacherová³

2010

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

468

347

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“…Diallyl sulfide (DAS), one of the component of garlic, has been reported to act as a potential chemopreventive function for many human cancer such as colon, lung, and skin (24). In our earlier studies, DAS inhibited the N-acetyltransferase activity in bacteria (25,26), human colon cancer cells (27) and bladder cancer cells (28), and inhibits murine WEHI-3 leukemia cells in BALB/c mice in vitro and in vivo (29). However, the mechanistic role of DAS-mediated apoptosis in human cervical cancer cells, especially the role of ER stress, caspases, and mitochondria in the induction of apoptosis remains limited.…”

Section: Introductionmentioning

confidence: 97%

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

Chung

Liu

et al. 2011

Int J Oncol

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Abstract. Diallyl sulfide (DAS), one of the main active constituents of garlic, causes growth inhibition of cancer cells in vitro and promotes immune responses in vivo in experimental settings. However, its effects on the induction of cell cycle and apoptosis in human cervical cancer cells are still unclear. The aims of this study were to explore the anti-cancer effects of DAS in HeLa human cervical cancer cells and to investigate the underlying mechanisms in vitro. Cytotoxicity and apoptosis in HeLa human cervical cancer cells were examined by the morphological changes, viability assay, 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining, comet assay, Western blotting and confocal microscopy examination. The results showed that DAS treatment for 24-72 h resulted in a marked decrease in cell viability time-and dose-dependently. Flow cytometric analysis showed that a 48-h treatment of 75 µM DAS induced G0/G1 cell cycle arrest and sub-G1 phase (apoptosis) in HeLa cells. Typical apoptotic nucleus alterations were observed by fluorescence microscopy in HeLa cells after exposure to DAS using DAPI staining. Cells treated with different concentrations of DAS also showed changes typical of apoptosis such as morphological changes, DNA damage and fragmentation, dysfunction of mitochondria, cytochrome c release and increased expression of pro-caspase-3 and -9. DAS also promoted the release of AIF and Endo G from mitochondria in HeLa cells. In conclusion, DAS induced G0/G1 cell cycle arrest and apoptosis in HeLa cells through caspase-and mitochondria and p53 pathways providing further understanding of the molecular mechanisms of DAS action in cervical cancer. This study, therefore, revealed that DAS significantly inhibits the growth and induces apoptosis of human cervical cancer HeLa cells in vitro.

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Effects of the Garlic Components Diallyl Sulfide and Diallyl Disulfide on Arylamine N-Acetyltransferase Activity in Human Colon Tumour Cells

Cited by 47 publications

References 31 publications

Allium vegetables and organosulfur compounds: do they help prevent cancer?

Allium vegetables and organosulfur compounds: do they help prevent cancer?

Phase Ii Drug Metabolizing Enzymes

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

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