Effects of the extracellular matrix on lumican expression in rat aortic smooth muscle cells <i>in vitro</i>

Qin, Hanjuan; Ishiwata, Toshiyuki; Asano, Gorō

doi:10.1002/path.994

Cited by 40 publications

(32 citation statements)

References 24 publications

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“…While endoglin is poorly expressed, lumican is highly expressed in the endothelium layer of a renal vein. In this tissue, staining of lumican in smooth muscle cells could also be detected, in agreement with previous reports (Chakravarti et al, 1998;Qin et al, 2001). These results provide evidence for an inverse correlation in the tissue distribution of endoglin and lumican in vivo.…”

Section: Inverse Correlationship In the Pattern Of Tissue Distributiosupporting

confidence: 93%

Lumican is down-regulated in cells expressing endoglin. Evidence for an inverse correlationship between Endoglin and Lumican expression

et al. 2004

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Section: Inverse Correlationship In the Pattern Of Tissue Distributiosupporting

confidence: 93%

Lumican is down-regulated in cells expressing endoglin. Evidence for an inverse correlationship between Endoglin and Lumican expression

et al. 2004

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“…Immunoblotting for lumican (Fig. 5C) revealed multiple diffuse bands predominantly from 50 -100 kDa, indicative of a proteoglycan form of lumican (33)(34)(35)(36). Consistent with the analysis of the MS signal intensities, there was significantly (p Ͻ 0.01) more lumican present in the intima of the atherosclerosis-prone internal carotid artery than in the atherosclerosis-resistant internal thoracic artery, with the mean value elevated by ϳ3-fold.…”

Section: Fig 2 Removal Of Thickened Intima For Proteomic Analysissupporting

confidence: 65%

Analysis of Intimal Proteoglycans in Atherosclerosis-prone and Atherosclerosis-resistant Human Arteries by Mass Spectrometry

Talusan

Bedri

Yang

et al. 2005

Molecular & Cellular Proteomics

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The propensity to develop atherosclerosis varies markedly among different sites in the human vasculature. To determine a possible cause for such differences in atherosclerosis susceptibility, a proteomics-based approach was used to assess the extracellular proteoglycan core protein composition of intimal hyperplasia from both the atherosclerosis-prone internal carotid artery and the atherosclerosis-resistant internal thoracic artery. The intimal proteoglycan composition in these preatherosclerotic lesions was found to be more complex than previously appreciated with up to eight distinct core proteins present, including the large extracellular proteoglycans versican and aggrecan, the basement membrane proteoglycan perlecan, the class I small leucine-rich proteoglycans biglycan and decorin, and the class II small leucine-rich proteoglycans lumican, fibromodulin, and prolargin/ PRELP (proline arginine-rich end leucine-rich repeat protein). Although most of these proteoglycans seem to be present in similar amounts at the two locations, there was a selective enhanced deposition of lumican in the intima of the atherosclerosis-prone internal carotid artery compared with the intima of the atherosclerosis-resistant internal thoracic artery. The enhanced deposition of lumican in the intima of an atherosclerosis prone artery has important implications for the pathogenesis of atherosclerosis.

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“…Northern Blot and in Situ Hybridization-Total RNA from the embryonic, larval, pupal, or adult stage of Drosophila was extracted using the TRIzol reagent (Invitrogen) (37). A total of 12.5 g of RNA was separated by electrophoresis on a formaldehyde-agarose gel and transferred overnight to a Hybond-N membrane (Amersham Biosciences).…”

Section: Expression Library Screening and Cloning Of Nip-the Drosophilamentioning

confidence: 99%

A Novel Transmembrane Protein Recruits Numb to the Plasma Membrane during Asymmetric Cell Division

Qin

Percival‐Smith

Liu

et al. 2004

Journal of Biological Chemistry

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Numb, an evolutionarily conserved cell fate-determining factor, plays a pivotal role in the development of Drosophila and vertebrate nervous systems. Despite lacking a transmembrane segment, Numb is associated with the cell membrane during the asymmetric cell division of Drosophila neural precursor cells and is selectively partitioned to one of the two progeny cells from a binary cell division. Numb contains an N-terminal phosphotyrosine-binding (PTB) domain that is essential for both the asymmetric localization and the fate specification function of Numb. We report here the isolation and characterization of a novel PTB domain-binding protein, NIP (Numb-interacting protein). NIP is a multipass transmembrane protein that contains two PTB domainbinding, NXXF motifs required for the interaction with Numb. In dividing Drosophila neuroblasts, NIP is colocalized to the cell membrane with Numb in a basal cortical crescent. Expression of NIP in Cos-7 cells recruited Numb from the cytosol to the plasma membrane. This recruitment of Numb to membrane by NIP was dependent on the presence of at least one NXXF site. In Drosophila Schneider 2 cells, NIP and Numb were colocalized at the plasma membrane. Inhibition of NIP expression by RNA interference released Numb to the cytosol. These results suggest that a direct protein-protein interaction between NIP and Numb is necessary and sufficient for the recruitment of Numb to the plasma membrane. Recruitment of Numb to a basal cortical crescent in a dividing neuroblast is essential for Numb to function as an intrinsic cell fate determinant.Asymmetric cell division, which can involve extrinsic and/or intrinsic factors, is a fundamental mechanism of generating cell diversity during the development of complex organisms (1, 2). Extrinsic factors such as Delta and its receptor Notch (3, 4) function in cell-cell communication to specify the fate of cells (5-7). Asymmetric determinants are intrinsic factors that are selectively segregated into one of the two daughter cells when a cell divides (2,8). Consequently, the sibling cell that inherits the asymmetric determinants adopts a different fate from the one that does not. Numb is a member of a growing family of proteins that also includes Prospero, Miranda, Inscuteable, and PON (partner of numb) (9 -14), which act as intrinsic determinants in asymmetric cell division. These proteins were identified through their requirement in the development of Drosophila peripheral and central nervous systems. The external sensory organ in Drosophila is composed of two outer (hair and socket) cells and three inner (sheath, glial, and neuron) cells, which are derived from a single sensory organ precursor through three consecutive asymmetric cell divisions. Numb is selectively partitioned to one of the two daughter cells at each binary division (15). Numb is also required for the development of the central nervous system (16 -18). During delaminating from the neuroectoderm and asymmetric division of a neuroblast, Numb, Prospero, and several other proteins...

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Effects of the extracellular matrix on lumican expression in rat aortic smooth muscle cells in vitro

Cited by 40 publications

References 24 publications

Lumican is down-regulated in cells expressing endoglin. Evidence for an inverse correlationship between Endoglin and Lumican expression

Lumican is down-regulated in cells expressing endoglin. Evidence for an inverse correlationship between Endoglin and Lumican expression

Analysis of Intimal Proteoglycans in Atherosclerosis-prone and Atherosclerosis-resistant Human Arteries by Mass Spectrometry

A Novel Transmembrane Protein Recruits Numb to the Plasma Membrane during Asymmetric Cell Division

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