Effects of the diet enrichment with β-glucan, sodium salt of butyric acid and vitamins on growth parameters and the profile of the gut microbiome of juvenile African catfish (Clarias gariepinus)

Arciuch-Rutkowska, Martyna; Nowosad, Joanna; Łuczyński, Michał Krzysztof; Jasiński, Szymon; Kucharczyk, Dariusz

doi:10.1016/j.anifeedsci.2024.115941

Cited by 3 publications

(3 citation statements)

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“…Feed was also prepared for the control group (C) enriched only with a mixture of oil and water (1:1, v:v) in the same proportion as in the research groups. The prepared feed was then dried at room temperature for 24 h [9,35].…”

Section: Experimental Feedmentioning

confidence: 99%

“…PCR reaction conditions were used according to the manufacturer's protocol. Sequencing was performed using paired-end (PE) technology, 2 × 300 nt, using the Illumina v3 kit (Illumina, San Diego, CA, USA) [35].…”

Section: Rna Extraction and Immune Related Genes Expressionmentioning

confidence: 99%

“…The inclusion of all the above ingredients in the fish diet, i.e., sodium butyrate, β-glucan and vitamins, is an important issue because there are no studies that describe the synergistic effect of this composition on breeding indicators and immune parameters of fish. Preliminary research conducted on African catfish fry (0.48 ± 0.17 g; DAH = 26 days) showed a positive effect of feed supplementation with compositions containing the above ingredients on the growth performance, survival rate and intestinal microbiome profile of the tested fish [35].…”

Section: Introductionmentioning

confidence: 97%

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Synergistic Effect of Dietary Supplementation with Sodium Butyrate, β-Glucan and Vitamins on Growth Performance, Cortisol Level, Intestinal Microbiome and Expression of Immune-Related Genes in Juvenile African Catfish (Clarias gariepinus)

Arciuch-Rutkowska,

Nowosad,

Gil

et al. 2024

IJMS

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The effect of dietary supplementation with sodium butyrate, β-glucan and vitamins (A, D3, E, K, C) on breeding indicators and immune parameters of juvenile African catfish was examined. The fish were fed with unenriched (group C) and enriched feed with a variable proportion of sodium butyrate/β-glucan, and constant content of vitamins (W1–W3). After the experiment, blood and the middle gut were collected. The microbiome of the gut was determined using Next Generation Sequencing (NGS). Liver tissue was collected for determination of expression of immune-related genes (HSP70, IL-1β, TNFα). W2 and W3 were characterized by the most favorable values of breeding indicators (p < 0.05). The highest blood cortisol concentration was in group C (71.25 ± 10.45 ng/mL), and significantly the lowest in W1 (46.03 ± 7.01 ng/ mL) (p < 0.05). The dominance of Cetobacterium was observed in all study groups, with the largest share in W3 (65.25%) and W1 (61.44%). Gene expression showed an increased number of HSP70 genes in W1. IL-1β and TNFα genes peaked at W3. The W3 variant turns out to be the most beneficial supplementation, due to the improvement of breeding and immunological parameters. The data obtained can be used to create a preparation for commercial use in the breeding of this species.

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Section: Experimental Feedmentioning

confidence: 99%

Section: Rna Extraction and Immune Related Genes Expressionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Synergistic Effect of Dietary Supplementation with Sodium Butyrate, β-Glucan and Vitamins on Growth Performance, Cortisol Level, Intestinal Microbiome and Expression of Immune-Related Genes in Juvenile African Catfish (Clarias gariepinus)

Arciuch-Rutkowska,

Nowosad,

Gil

et al. 2024

IJMS

Self Cite

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Role of β‐glucan on finfish and shellfish health and well‐being: A systematic review and meta‐analysis

Van Doan,

Sumon,

Tran

et al. 2024

Reviews in Aquaculture

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This study aimed to conduct a systematic review and meta‐analysis evaluating the inclusion of β‐glucan in aquaculture animal diets and its impact on their health outcomes. Relevant studies were identified from Scopus and Web of Science databases. A total of 82 primary studies published between 1996 and 2024 were reviewed, of which 70 were included in the meta‐analysis. The results revealed that the application of β‐glucan to aquaculture animal's diets significantly enhanced specific growth rate (SGR; mean effect, g = 2.71; p < 0.001), feed conversion ratio (FCR; g = −3.88; p < 0.0001) and lowered mortality after exposure to pathogens. Likewise, β‐glucan had a positive influence (p < 0.0001) on innate immune parameters (lysozyme and phagocyte activity, NBT, ACH50, and IgM). The study found that the effects of β‐glucans varied among marine and freshwater fish where freshwater fishes (g = 2.05–6.57) exhibit better performance. This study also found a negative correlation between fish's innate immune response and trophic level, suggesting that fish with higher trophic levels may be less efficient at absorbing this bio‐stimulant. Even though there were high heterogeneity (I2 = 73%–97%, p < 0.05) due to the diversity of tested organisms and publication bias, our model and findings are valid. The findings suggest that the dietary application of β‐glucans can have beneficial effects on growth and immune responses especially for freshwater species. The validity of these observations needs to be confirmed by further prospective studies.

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Next-Generation Sequencing to Determine Changes in the Intestinal Microbiome of Juvenile Sturgeon Hybrid (Acipenser gueldenstaedtii♀ × Acipenser baerii♂) Resulting from Sodium Butyrate, Β-Glucan and Vitamin Supplementation

Arciuch-Rutkowska,

Nowosad,

Łuczyński

et al. 2024

Genes

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Background/Objectives: The effect of sodium butyrate (NaB), β-glucan (βG) and vitamins in the diet on gut microbiome, cortisol level, lysozyme activity and growth parameters of juvenile hybrid sturgeon (Acipenser gueldenstaedtii♀ × Acipenser baerii♂) was determined. Methods: Sturgeon hybrids (n = 144) were divided into three groups with enriched feeding (mg/kg of feed): FQV1 (50 NaB; 20 βG; const. vitamins), FQV2 (150 NaB; 20 βG; const. vitamins), FQV3 (50 NaB; 60 βG; const. vitamins) and control (not supplemented), each group in triplicate, 12 fish in each repetition. Rearing was carried out for 30 days in controlled conditions. Gut microbiome was characterized using Next Generation Sequencing (NGS) of DNA samples isolated from intestinal content. Cortisol level was determined using the ELISA test. Lysozyme activity was measured by turbidimetric test. Results: Based on data obtained from NGS, it was determined that the FQV1 group is characterized by the highest values of diversity indices (Shannon, Simpson and Chao-1) and the largest number of ASVs (Amplicon Sequence Variants). The highest abundance of probiotic bacteria (Lactobacillus, Lactococcus) was determined in the FQV1 group. The highest cortisol concentration was determined in the control (33.26 ng/mL), while the lowest was in FQV3 (27.75 ng/mL). The highest lysozyme activity was observed in FQV1 (154.64 U/mL), and the lowest in FQV2 (104.39 U/mL) and control (121.37 U/mL) (p < 0.05). FQV2 was characterized by significantly more favorable values of breeding indicators (p < 0.05). Conclusions: The obtained results prove that an appropriate composition of NaB, βG and vitamins can be used in the commercial breeding of juvenile hybrid sturgeons.

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Effects of the diet enrichment with β-glucan, sodium salt of butyric acid and vitamins on growth parameters and the profile of the gut microbiome of juvenile African catfish (Clarias gariepinus)

Cited by 3 publications

References 58 publications

Synergistic Effect of Dietary Supplementation with Sodium Butyrate, β-Glucan and Vitamins on Growth Performance, Cortisol Level, Intestinal Microbiome and Expression of Immune-Related Genes in Juvenile African Catfish (Clarias gariepinus)

Synergistic Effect of Dietary Supplementation with Sodium Butyrate, β-Glucan and Vitamins on Growth Performance, Cortisol Level, Intestinal Microbiome and Expression of Immune-Related Genes in Juvenile African Catfish (Clarias gariepinus)

Role of β‐glucan on finfish and shellfish health and well‐being: A systematic review and meta‐analysis

Next-Generation Sequencing to Determine Changes in the Intestinal Microbiome of Juvenile Sturgeon Hybrid (Acipenser gueldenstaedtii♀ × Acipenser baerii♂) Resulting from Sodium Butyrate, Β-Glucan and Vitamin Supplementation

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