[Purpose] This study aimed to determine whether muscle atrophy induced by ischemic
reperfusion injury in rats can be prevented by the administration of antioxidants and
exercise. [Subjects] Rats were randomly divided into five groups: non-treated, ischemic,
exercise, ascorbic acid and exercise, and tocopherol and exercise. [Methods] The relative
weight ratio of the soleus muscle and the length of the soleus muscle fiber cross-section
minor axis were used for the evaluation of muscle atrophy. Pain was assessed as the
weight-bearing ratio of the ischemic side. A multiple comparison test and the paired
t-test were used for the statistical analyses. [Results] Compared with the non-treated
group, the relative weight ratios of the soleus muscle and the lengths of the soleus
muscle fiber cross-section minor axis significantly decreased in the other groups.
Excluding the non-treated group, the relative weight ratios of the soleus muscle were
heaviest in the tocopherol and exercise group. Excluding the non-treated group, the
lengths of the soleus muscle fiber cross-section minor axis were longest in the tocopherol
and exercise group, followed by the ischemic, exercise, and ascorbic acid and exercise
groups. The amount of antioxidant substances did not decrease on the weight-bearing ratio
of the ischemic side. [Conclusion] In this study, using an experimental rat model, we
confirmed that antioxidants and exercise effect muscle atrophy induced by ischemic
reperfusion. The results show that muscle regeneration was facilitated by phagocytosis in
the tocopherol and exercise group.