Effects of the Bone/Bone Marrow Microenvironments on Prostate Cancer Cells and CD59 Expression

Yan, Bo; Li, Yan; Min, Shaoju; Zhang, Peng; Xu, Bin; Wang, Zhen; Zhang, Wei; Chen, Jiasheng; Luo, Guangheng; Liu, Chunxiao

doi:10.1155/2020/2753414

Cited by 5 publications

(3 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TRAF6, a major signal transducer in RANKL-activated OCG, essentially helps to activate some pivotal intracellular signaling pathways responsible for the regulation of osteoclast formation and function, namely, NF-κB and MAPKs (JNK, ERK, and p 38). , The NF-κB signaling pathway facilitates osteoclast formation, osteoclast function, and bone resorption after RANKL-to-RANK binding . An inactive form of NF-κB that is normally complexed with inhibitory protein IκBα is typically located in the cytoplasm during the resting state of the cell.…”

Section: Discussionmentioning

confidence: 99%

Corynoline Suppresses Osteoclastogenesis and Attenuates ROS Activities by Regulating NF-κB/MAPKs and Nrf2 Signaling Pathways

Jin,

Yu,

Yang

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Declining estrogen production in postmenopausal females causes osteoporosis in which the resorption of bone exceeds the increase in bone formation. Although clinical drugs are currently available for the treatment of osteoporosis, sustained medication use is accompanied by serious side effects. Corydalis bungeana Herba, a famous traditional Chinese herb listed in the Chinese Pharmacopoeia Commission, constitutes various traditional Chinese Medicine prescriptions, which date back to thousands of years. One of the primary active components of C. bungeana Turcz. is Corynoline (Cor), a plant isoquinoline alkaloid derived from the Corydalis species, which possesses bone metabolism disease therapeutic potential. The study aimed at exploring the effects as well as mechanisms of Cor on osteoclast formation and bone resorption. TRAcP staining, F-actin belt formation, and pit formation were employed for assessing the osteoclast function. Western blot, qPCR, network pharmacology, and docking analyses were used for analyzing the expression of osteoclast-associated genes and related signaling pathways. The study focused on investigating how Cor affected OVX-induced trabecular bone loss by using a mouse model. Cor could weaken osteoclast formation and function by affecting the biological receptor activators of NF-κB and its ligand at various concentrations. Mechanistically, Cor inhibited the NF-κB activation, and the MAPKs pathway stimulated by RANKL. Besides, Cor enhanced the protein stability of the Nrf2, which effectively abolished the RANKL-stimulated ROS generation. According to an OVX mouse model, Cor functions in restoring bone mass, improving microarchitecture, and reducing the ROS levels in the distal femurs, which corroborated with its in vitro antiosteoclastogenic effect. The present study indicates that Cor may restrain osteoclast formation and bone loss by modulating NF-κB/MAPKs and Nrf2 signaling pathways. Cor was shown to be a potential drug candidate that can be utilized for the treatment of osteoporosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Corynoline Suppresses Osteoclastogenesis and Attenuates ROS Activities by Regulating NF-κB/MAPKs and Nrf2 Signaling Pathways

Jin,

Yu,

Yang

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Among these, a consistent set of 12 proteins associated with osteogenesis were identified across all samples, including ALB [ 35 ], ITGB1 [ 36 ], VIM [ 37 ], MSN [ 38 ], MYH10 [ 39 ], HADHA [ 40 ], GSS [ 41 ], COPG1 [ 42 ], SAMHD1 [ 43 ], DDX1 [ 44 ], PSMD9 [ 45 ], and MFGE8 [ 46 ]. Conversely, a subset of 10 proteins consistently implicated in osteoclastogenesis at both time points were identified, namely CDC37 [ 47 ], GNAT3 [ 48 ], PSAP [ 49 ], HMGB1 [ 50 ], CD59 [ 51 ], YBX3 [ 52 ] H3C1 [ 53 ], NLRP9 [ 54 ], CHMP5 [ 55 ], and DYNLL2 [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of Resolvin-E1 and Maresin-1 on Bone Marrow Stem Cell Osteogenesis under Inflammatory Stress

AlZahrani,

Shinwari,

Alaiya

et al. 2024

Cells

View full text Add to dashboard Cite

Periodontal disease is characterized by inflammation and bone loss. Central to its pathogenesis is the dysregulated inflammatory response, complicating regenerative therapies. Mesenchymal stem cells (MSCs) hold significant promise in tissue repair and regeneration. This study investigated the effects of specialized pro-resolving mediators (SPMs), Resolvin E1 (RvE1) and Maresin 1 (MaR1), on the osteogenic differentiation of human bone marrow-derived MSCs under inflammatory conditions. The stem cells were treated with SPMs in the presence of lipopolysaccharide (LPS) to simulate an inflammatory environment. Osteogenic differentiation was assessed through alkaline phosphatase activity and alizarin red staining. Proteomic analysis was conducted to characterize the protein expression profile changes, focusing on proteins related to osteogenesis and osteoclastogenesis. Treatment with RvE1 and MaR1, both individually and in combination, significantly enhanced calcified deposit formation. Proteomic analysis revealed the differential expression of proteins associated with osteogenesis and osteoclastogenesis, highlighting the modulatory impact of SPMs on bone metabolism. RvE1 and MaR1 promote osteogenic differentiation of hBMMSCs in an inflammatory environment, with their combined application yielding synergistic effects. This study provides insights into the therapeutic potential of SPMs in enhancing bone regeneration, suggesting a promising avenue for developing regenerative therapies for periodontal disease and other conditions characterized by inflammation-induced bone loss.

show abstract

“…The results indicated that these MSCs can alter the phenotype of PC3 cells and increase their expression of CD59 by activating the RANK/RANKL/OPG signaling pathway, which makes them less detectable by the immune system. However, since this was an in vitro model, it may not fully replicate the complex interactions between human BM‐MSCs, osteoblasts, and PCa cells (Yan et al, 2020). In rabbit models of BCa, the implanted BM‐MSCs can differentiate into vascular endothelial cells and stimulate angiogenesis in the tumor microenvironment, which may be the main pathway for promoting tumor growth (Zhang et al, 2010).…”

Section: The Beneficial Characteristics Of Mscsmentioning

confidence: 99%

Mesenchymal stem cells and their extracellular vesicles in urological cancers: Prostate, bladder, and kidney

Saadh,

Alhuthali,

Gonzales Aníbal

et al. 2023

Cell Biology International

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are recognized for their remarkable ability to differentiate into multiple cell types. They are also known to possess properties that can fight cancer, leading to attempts to modify MSCs for use in anticancer treatments. However, MSCs have also been found to participate in pathways that promote tumor growth. Many studies have been conducted to explore the potential of MSCs for clinical applications, but the results have been inconclusive, possibly due to the diverse nature of MSC populations. Furthermore, the conflicting roles of MSCs in inhibiting tumors and promoting tumor growth hinder their adaptation to anticancer therapies. Antitumorigenic and protumorigenic properties of MSCs in urological cancers such as bladder, prostate, and renal are not as well established, and data comparing them are still limited. MSCs hold significant promise as a vehicle for delivering anticancer agents and suicide genes to tumors. Presently, numerous studies have concentrated on the products derived from MSCs, such as extracellular vesicles (EVs), as a form of cell‐free therapy. This work aimed to review and discuss the current knowledge of MSCs and their EVs in urological cancer therapy.

show abstract

Effects of the Bone/Bone Marrow Microenvironments on Prostate Cancer Cells and CD59 Expression

Cited by 5 publications

References 51 publications

Corynoline Suppresses Osteoclastogenesis and Attenuates ROS Activities by Regulating NF-κB/MAPKs and Nrf2 Signaling Pathways

Corynoline Suppresses Osteoclastogenesis and Attenuates ROS Activities by Regulating NF-κB/MAPKs and Nrf2 Signaling Pathways

Impact of Resolvin-E1 and Maresin-1 on Bone Marrow Stem Cell Osteogenesis under Inflammatory Stress

Mesenchymal stem cells and their extracellular vesicles in urological cancers: Prostate, bladder, and kidney

Contact Info

Product

Resources

About