The influences of austenitization temperature and holding time on transformation plasticity behaviors were experimentally investigated in a three-point bending test, wherein the test specimen is simply supported from one end and fixed at the opposite end. The specimens were fabricated from S45C steel and heated to different austenitization temperatures (850, 900, and 950 °C). The temperatures of the specimens in the austenite phase were kept constant for 5 or 20 min, and then naturally cooled to room temperature. During the cooling process, the specimens were subjected to the maximum bending stress (less than the yield stress) before the transformation start point (around 800 °C). Under the bending stress, the transformation plasticity due to austenite to pearlite occurred. From the measured maximum transformation plasticity deflections, the transformation plasticity coefficients were determined. These coefficients were found to strongly depend on the austenitization temperature and holding time.