Effects of the antioxidants curcumin and vitamin C on cisplatin-induced clastogenesis in Wistar rat bone marrow cells

Antunes, Lusânia Maria Greggi; Araújo, Maria Cristina P.; Darin, Joana D’Árc Castania; Bianchi, María de Lourdes Pires

doi:10.1016/s1383-5718(99)00220-x

Cited by 96 publications

(48 citation statements)

References 31 publications

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“…This antitumor drug is usually selected as a positive control for short-term antimutagenicity tests. Our data support the literature reports that show an increased frequency of chromosomal damage in rodent bone marrow cells after administrations of cDDP (Antunes et al 2000;Mora et al, 2002).…”

Section: Discussionsupporting

confidence: 92%

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In vivo Cytogenetic Effects of Multiple Doses of Dietary Vegetable Oils

Antunes¹,

Bianchi²

2010

Olives and Olive Oil in Health and Disease Prevention

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Polyphenols are potent antioxidants that are particularly abundant in the Mediterranean diet, with olive oil being the main fat source. A number of investigations have reported that phenolic compounds found in dietary oils are antioxidants and could provide protective effects by inhibiting DNA oxidative damage. However, few studies have been published on the biological activity of vegetable oils, including their possible mutagenic/antimutagenic effects. The objective of the current study was to investigate the cytogenetic effects of multiple doses of four vegetable oils in rat bone marrow cells and to examine the possible antimutagenic effects of these oils in chromosomal damage induced by the antitumor drug cisplatin. These oils are consumed by humans and commonly used as drug vehicles. The rats received treatment with multiple doses of canola oil, olive oil, virgin olive oil, and corn oil (5 mL kg -1 ) alone or combined with the antitumor drug cisplatin (5 mg kg -1 ). Treatments with vegetable oils alone did not increase the percentage of cells with chromosomal aberrations (p > 0.05). Olive, virgin olive and canola oils showed protective effects against cisplatin-induced chromosomal damage (p < 0.05). A rational mechanism for the protective effects of vegetable oils is that their phenolic compounds have antioxidant and antimutagenic properties in vivo.

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Section: Discussionsupporting

confidence: 92%

“…The dose of the oils tested was selected on the basis of preliminary results with single administration (Evangelista et al, 2004). The dose of cDDP was the same as used by Antunes et al (2000). In addition to these groups, one set of rats was treated with three administrations of distilled water and 5 mg kg -1 b.w.…”

Section: Chromosomal Aberrations Assaymentioning

confidence: 99%

In vivo Cytogenetic Effects of Multiple Doses of Dietary Vegetable Oils

Antunes¹,

Bianchi²

2010

Olives and Olive Oil in Health and Disease Prevention

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“…Vitamin C also has in vivo anticlastogenic effects against chromosomal damage induced by cisplatin in rodents. [45] However, few papers have reported on the effects of this vitamin in cisplatintreated rats, and usually single dose has been used.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Vitamin C on Cisplatin-Induced Renal Damage: A Light and Electron Microscopic Study

2008

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The present study was performed to investigate whether the chronic administration of antioxidant vitamin C provided morphological protection on cisplatin-induced renal damage. Wistar albino male rats were divided into control and two experiment groups, each consisting of six rats. Cisplatin (5 mg/kg/ month) was administered intravenously to the second and third group for three months. After the first application of cisplatin, vitamin C (8 mg/kg/day) to the third group was administered intramuscular for 3 months. At the end of the third month, the kidney specimens of the all groups were obtained. All of these kidney specimens were processed for light and electron microscopical examination. In the second group, most of the renal corpuscle lost their normal appearance and size, especially in the corticomedullary region. The most obvious changes were encountered in the proximal tubules. These changes were tubular dilation, thickening of basement membrane, loss of brush border, vacuolization, and swollenness of mitochondria in the proximal tubule epithelial cells. In addition, infiltration foci were observed mainly in the cortical region. In the third group, which was administered cisplatin plus vitamin C, although the structural damages and morphometric changes were lessened, mononuclear cell infiltration was still observed. This study suggests that the chronic administration of vitamin C may be of therapeutic benefit on cisplatin nephrotoxicity.

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“…Several experimental and clinical studies indicate that curcumin exhibits antioxidant, anti-inflammatory, and anticancer properties (20,23,49). It has also been reported that the use of commercially available curcumin supplements (2 -4 g/d) or recommended therapeutic oral dose of 6 to 8 g/d results in micromolar concentrations of curcuminoids in the gastrointestinal tract (50 -55).…”

Section: Discussionmentioning

confidence: 99%

Modulation of the function of the multidrug resistance–linked ATP-binding cassette transporter ABCG2 by the cancer chemopreventive agent curcumin

Chearwae

Shukla

Limtrakul

et al. 2006

Molecular Cancer Therapeutics

110

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Curcumin (curcumin I), demethoxycurcumin (curcumin II), and bisdemethoxycurcumin (curcumin III) are the major forms of curcuminoids found in the turmeric powder, which exhibit anticancer, antioxidant, and anti-inflammatory activities. In this study, we evaluated the ability of purified curcuminoids to modulate the function of either the wild-type 482R or the mutant 482T ABCG2 transporter stably expressed in HEK293 cells and drug-selected MCF-7 FLV1000 and MCF-7 AdVp3000 cells. Curcuminoids inhibited the transport of mitoxantrone and pheophorbide a from ABCG2-expressing cells. However, both cytotoxicity and [ 3 H]curcumin I accumulation assays showed that curcuminoids are not transported by ABCG2. Nontoxic concentration of curcumin I, II, and III sensitized the ABCG2-expressing cells to mitoxantrone, topotecan, SN-38, and doxorubicin. This reversal was not due to reduced expression because ABCG2 protein levels were unaltered by treatment with 10 Mmol/L curcuminoids for 72 hours. Curcumin I, II, and III stimulated (2.4-to 3.3-fold) ABCG2-mediated ATP hydrolysis and the IC 50 s were in the range of 7.5 to 18 nmol/L, suggesting a high affinity of curcuminoids for ABCG2. Curcuminoids also inhibited the photolabeling of ABCG2 with P]8-azidoATP to ABCG2, suggesting that they do not interact with the ATP-binding site of the transporter. Collectively, these data show that, among curcuminoids, curcumin I is the most potent modulator of ABCG2 and thus should be considered as a treatment to increase the efficacy of conventional chemotherapeutic drugs.

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Effects of the antioxidants curcumin and vitamin C on cisplatin-induced clastogenesis in Wistar rat bone marrow cells

Cited by 96 publications

References 31 publications

In vivo Cytogenetic Effects of Multiple Doses of Dietary Vegetable Oils

In vivo Cytogenetic Effects of Multiple Doses of Dietary Vegetable Oils

Protective Effects of Vitamin C on Cisplatin-Induced Renal Damage: A Light and Electron Microscopic Study

Modulation of the function of the multidrug resistance–linked ATP-binding cassette transporter ABCG2 by the cancer chemopreventive agent curcumin

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