Effects of the activated mitogen-activated protein kinase pathway via the c-ros receptor tyrosine kinase on the T47D breast cancer cell line following alcohol exposure

Lee, Hyung Tae; Kim, Se Kye; Choi, Mi Ran; Park, Ji Hyun; Jung, Kyoung Hwa; Chai, Young Gyu

doi:10.3892/or.2012.2209

Cited by 7 publications

(8 citation statements)

References 38 publications

(50 reference statements)

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“…The cell cycle assay was performed as previously described (17). The cells were plated in 10-cm 2 plates (Corning Inc., Corning, NY, USA) and cultured in normal growth medium for 24 h before treatment with pargyline or tranylcypromine (Sigma-Aldrich, St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Effects of the monoamine oxidase inhibitors pargyline and tranylcypromine on cellular proliferation in human prostate cancer cells

et al. 2013

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Chemotherapy is one of the therapeutic strategies that has been used for the inhibition of cancer cell proliferation in several types of cancer, including prostate cancer. Although monoamine oxidase (MAO) inhibitors, phytoestrogen and antioxidants used in chemotherapy have been systematically studied, their effects on cancer cell growth remain to be fully understood. The purpose of this study was to investigate the effects of the MAO inhibitors, pargyline and tranylcypromine on cell survival in human prostate carcinoma (LNCaP-LN3) cells. After treating LNCaP-LN3 cells with pargyline or tranylcypromine, we examined cell proliferation, cell cycle pattern, apoptosis and the expression levels of apoptosis-related genes. The proliferation of cells exposed to pargyline decreased in a dose- and time-dependent manner, while tranylcypromine-treated cells showed the opposite results. Treatment with pargyline significantly induced cell cycle arrest at the G1 phase compared to the control and tranylcypromine-treated cells. In addition, pargyline induced an increase in the cell death rate by promoting apoptosis; however, tranylcypromine had no effect on LNCaP-LN3 cells. Based on our results, we suggest that pargyline is more powerful than tranylcypromine for the treatment of human prostate cancer.

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Section: Methodsmentioning

confidence: 99%

Effects of the monoamine oxidase inhibitors pargyline and tranylcypromine on cellular proliferation in human prostate cancer cells

et al. 2013

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“…JNKs have been proposed to function both as tumor suppressors and mediators of many malignant cell proliferation, survival and resistance to chemotherapy, such as glioma (Wagner et al, 2009), breast cancer (Lee et al, 2013). However, the functional role of JNK in breast cancer after treated with GLA has not been investigated.…”

Section: Glaucocalyxin a Activates Fasl And Induces Apoptosis Throughmentioning

confidence: 99%

Glaucocalyxin A Activates FasL and Induces Apoptosis Through Activation of the JNK Pathway in Human Breast Cancer Cells

Jiang²,

Z³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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This study was conducted to analyze the molecular mechanisms responsible for anti-proliferation effects of glaucocalyxin A in cultured MCF-7 and Hs578T breast cancer cells. The concentration that reduced cell viability to 50% (IC50) after 72 h treatment was derived and potential molecular mechanisms of anti-proliferation using the IC50 were investigated as changes in cell cycle arrest and apoptosis. Gene and protein expression changes related to apoptosis were investigated by semi-quantitative RT-PCR and western blotting, respectively. Involvement of phosphorylated mitogen-activated protein kinases and JNK signaling in regulation of these molecules was characterized by western blotting. Cell viability decreased in a concentration-dependent manner and the IC50 was determined as 1 μΜ in MCF-7 and 4 μΜ in Hs578T cell. Subsequently, we demonstrated that the GLA-induced MCF-7 and Hst578T cell death was due to cell cycle arrest at the G2/M transition and was associated with activation of the c-jun N-terminal kinase (JNK) pathway. We conclude that GLA has the potential to inhibit the proliferation of human breast cancer cells through the JNK pathway and suggest its application forthe effective therapy for patients with breast cancer.

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“…As breast cancer, compared with other cancers affecting women, is associated with alcohol consumption, a related study (Lee et al, 2013) investigated the effect of alcohol exposure (100 mM for 48 h) on the growth regulation in T47D breast cancer cells. The researchers used 2D electrophoresis and matrix assisted laser desorption, time of flight (MALDI-ToF) MS to evaluate changes in protein expression in addition to various other techniques.…”

Section: Proteomic Profiling Studies In Cell Culturesmentioning

confidence: 99%

Proteomic Approaches and Identification of Novel Therapeutic Targets for Alcoholism

Gorini

Harris

Mayfield

2013

Neuropsychopharmacol

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Recent studies have shown that gene regulation is far more complex than previously believed and does not completely explain changes at the protein level. Therefore, the direct study of the proteome, considerably different in both complexity and dynamicity to the genome/transcriptome, has provided unique insights to an increasing number of researchers. During the past decade, extraordinary advances in proteomic techniques have changed the way we can analyze the composition, regulation, and function of protein complexes and pathways underlying altered neurobiological conditions. When combined with complementary approaches, these advances provide the contextual information for decoding large data sets into meaningful biologically adaptive processes. Neuroproteomics offers potential breakthroughs in the field of alcohol research by leading to a deeper understanding of how alcohol globally affects protein structure, function, interactions, and networks. The wealth of information gained from these advances can help pinpoint relevant biomarkers for early diagnosis and improved prognosis of alcoholism and identify future pharmacological targets for the treatment of this addiction.

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Effects of the activated mitogen-activated protein kinase pathway via the c-ros receptor tyrosine kinase on the T47D breast cancer cell line following alcohol exposure

Cited by 7 publications

References 38 publications

Effects of the monoamine oxidase inhibitors pargyline and tranylcypromine on cellular proliferation in human prostate cancer cells

Effects of the monoamine oxidase inhibitors pargyline and tranylcypromine on cellular proliferation in human prostate cancer cells

Glaucocalyxin A Activates FasL and Induces Apoptosis Through Activation of the JNK Pathway in Human Breast Cancer Cells

Proteomic Approaches and Identification of Novel Therapeutic Targets for Alcoholism

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