Effects of the acid-tolerant engineered bacterial strain Megasphaera elsdenii H6F32 on ruminal pH and the lactic acid concentration of simulated rumen acidosis in vitro

Long, Miao; Feng, Wenjing; Li, P.; Zhang, Y.; He, R.X.; Yu, Li-Hui; He, Jianbin; Wang, Jing; Li, Y.M.; Wang, Z.; Liu, G.W.

doi:10.1016/j.rvsc.2013.11.013

Cited by 18 publications

(12 citation statements)

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“…The diet is known to alter the composition of the rumen microbiota [14, 15]. Specific feed supplements have been the object of experimental trials on the rumen microbiome: these include canola [16], probiotic bacteria [17], organic acids [18, 19]. Mostly, feed supplementations had the objective of counteracting the effects of the high-energy diets typical of dairy cows on the rumen pH, the composition of the rumen microbiota, and the health of the animals.…”

Section: Introductionmentioning

confidence: 99%

Rumen microbiome in dairy calves fed copper and grape-pomace dietary supplementations: Composition and predicted functional profile

et al. 2018

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The rumen microbiome is fundamental for the productivity and health of dairy cattle and diet is known to influence the rumen microbiota composition. In this study, grape-pomace, a natural source of polyphenols, and copper sulfate were provided as feed supplementation in 15 Holstein-Friesian calves, including 5 controls. After 75 days of supplementation, genomic DNA was extracted from the rumen liquor and prepared for 16S rRNA-gene sequencing to characterize the composition of the rumen microbiota. From this, the rumen metagenome was predicted to obtain the associated gene functions and metabolic pathways in a cost-effective manner. Results showed that feed supplementations did alter the rumen microbiome of calves. Copper and grape-pomace increased the diversity of the rumen microbiota: the Shannon’s and Fisher’s alpha indices were significantly different across groups (p-values 0.045 and 0.039), and Bray-Curtis distances could separate grape-pomace calves from the other two groups. Differentially abundant taxa were identified: in particular, an uncultured Bacteroidales UCG-001 genus and OTUs from genus Sarcina were the most differentially abundant in pomace-supplemented calves compared to controls (p-values 0.003 and 0.0002, respectively). Enriched taxonomies such as Ruminiclostridium and Eubacterium sp., whose functions are related to degradation of the grape- pomace constituents (e.g. flavonoids or xyloglucan) have been described (p-values 0.027/0.028 and 0.040/0.022 in Pomace vs Copper and Controls, respectively). The most abundant predicted metagenomic genes belonged to the arginine and proline metabolism and the two- component (sensor/responder) regulatory system, which were increased in the supplemented groups. Interestingly, the lipopolysaccharide biosynthetic pathway was decreased in the two supplemented groups, possibly as a result of antimicrobial effects. Methanogenic taxa also responded to the feed supplementation, and methane metabolism in the rumen was the second most different pathway (up-regulated by feed supplementations) between experimental groups.

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Section: Introductionmentioning

confidence: 99%

Rumen microbiome in dairy calves fed copper and grape-pomace dietary supplementations: Composition and predicted functional profile

et al. 2018

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“…In addition the use of feed supplements to enhance the rumen microbial community and subsequently ruminal fermentation has also been suggested for this purpose. These supplements include the use of yeasts, like Saccharomyces cerevisiae , strain CNCM I-1077 [ 14 ], probiotic bacteria, like Megasphaera elsdenii , strain H6F32 [ 15 ], and dicarboxylic acids [ 16 ], flavonoids [ 17 ] and essential oils [ 18 ]. It has been suggested that the dicarboxylic acids malate and fumarate attenuate the ruminal pH drop during high grain feeding [ 16 ], and that these acids increase the activity of the succinate-propionate metabolic pathway in several rumen bacteria, resulting in increased lactic acid uptake and production of propionate [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis of rumen microbial population in dairy heifers fed a high grain diet supplemented with dicarboxylic acids or polyphenols

Nardi

Marchesini

et al. 2016

BMC Vet Res

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BackgroundThe aim of this study was to investigate the effects of two feed supplements on rumen bacterial communities of heifers fed a high grain diet. Six Holstein-Friesian heifers received one of the following dietary treatments according to a Latin square design: no supplement (control, C), 60 g/day of fumarate-malate (organic acid, O) and 100 g/day of polyphenol-essential oil (P). Rumen fluid was analyzed to assess the microbial population using Illumina sequencing and quantitative real time PCR.ResultsThe P treatment had the highest number of observed species (P < 0.10), Chao1 index (P < 0.05), abundance based coverage estimated (ACE) (P < 0.05), and Fisher’s alpha diversity (P < 0.10). The O treatment had intermediate values between C and P treatments with the exception of the Chao1 index. The PCoA with unweighted Unifrac distance showed a separation among dietary treatments (P = 0.09), above all between the C and P (P = 0.05). The O and P treatments showed a significant increase of the family Christenenellaceae and a decline of Prevotella brevis compared to C. Additionally, the P treatment enhanced the abundance of many taxa belonging to Bacteroidetes, Firmicutes and Tenericutes phyla due to a potential antimicrobial activity of flavonoids that increased competition among bacteria.ConclusionsOrganic acid and polyphenols significantly modified rumen bacterial populations during high-grain feeding in dairy heifers. In particular the polyphenol treatment increased the richness and diversity of rumen microbiota, which are usually high in conditions of physiological rumen pH and rumen function.

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“…Good management and nutritional strategies alone [ 8 , 5 ] are not always enough to avoid the onset of SARA in high yielding dairy cows and for this reason other preventative measures have been suggested. This included physical manipulation of fiber and grain particle size [ 8 , 11 , 12 ], the inclusion of antibiotics in the diet [ 13 ], the use of yeasts and probiotic bacteria [ 14 , 15 ], and the addition of dicarboxylic acids [ 16 ], flavonoids [ 17 ] or essential oils [ 18 ] to manipulate rumen microbial communities and subsequently ruminal fermentation.…”

Section: Introductionmentioning

confidence: 99%

Use of dicarboxylic acids and polyphenols to attenuate reticular pH drop and acute phase response in dairy heifers fed a high grain diet

et al. 2014

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BackgroundThe aim of this study was to determine the ability of two feed additives, a fumarate-malate (FM) and a polyphenol-essential oil mixture (PM), in attenuating the drop of ruminal pH and the metabolic and immune response resulting from an excessively high grain diet. Six heifers were used in a 3 × 3 Latin square experiment and fed a low starch (LS) diet for 14 d, followed by a high starch (HS) diet for 8 d (NDF 33.6%, starch 30.0% DM). In the last 5 days of each period, barley meal was added to decrease rumen pH. During HS feeding all animals were randomly assigned to one of the following three dietary treatments: no supplement/control (CT), a daily dose of 60 g/d of FM, or 100 g/d of PM. Reticular pH was continuously recorded using wireless boluses. On d 21 of each period, rumen fluid was collected by rumenocentesis (1400 h), together with blood (0800 h) and fecal samples (0800, 1400, and 2100 h).ResultsThe correlation coefficient of pH values obtained using the boluses and rumenocentesis was 0.83. Compared with CT and PM, the FM treatment led to a lower DMI. Nadir pH was lowest during CT (5.40, 5.69, and 5.62 for CT, FM and PM, respectively), confirming the effectiveness of both supplements in reducing the pH drop caused by high grain feeding. This result was confirmed by the highest average time spent daily below 5.6 pH (199, 16 and 18 min/d) and by the highest acetate to propionate ratio of the CT fed heifers. The PM decreased the concentrations of neutrophils (2.9, 3.2, and 2.8 109/L) and acute phase proteins: SAA (37.1, 28.6 and 20.1 μg/mL), LBP (4.1, 3.8, and 2.9 μg/mL), and Hp (675, 695 and 601 μg/mL). Free lipopolysaccharides (LPS) were detected in blood and feces, but their concentrations were not affected by treatments, as the remaining blood variables.ConclusionsData suggest that both additives could be useful in attenuating the effects of excessive grain feeding on rumen pH, but the PM supplement was more effective than FM in reducing the inflammatory response compared to CT.

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Effects of the acid-tolerant engineered bacterial strain Megasphaera elsdenii H6F32 on ruminal pH and the lactic acid concentration of simulated rumen acidosis in vitro

Cited by 18 publications

References 5 publications

Rumen microbiome in dairy calves fed copper and grape-pomace dietary supplementations: Composition and predicted functional profile

Rumen microbiome in dairy calves fed copper and grape-pomace dietary supplementations: Composition and predicted functional profile

Metagenomic analysis of rumen microbial population in dairy heifers fed a high grain diet supplemented with dicarboxylic acids or polyphenols

Use of dicarboxylic acids and polyphenols to attenuate reticular pH drop and acute phase response in dairy heifers fed a high grain diet

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