Effects of Thalassophryne nattereri fish venom in isolated perfused rat kidney

Facó, Patrícia Emília; Havt, Alexandre; Barbosa, Paula; Nobre, Arlandia Cristina Lima; Bezerra, Glaydcianne Pinheiro; Menezes, Dalgimar Beserra de; Fonteles, M. C.; Lopes-Ferreira, Mônica; Monteiro, Helena Serra Azul

doi:10.1016/s0041-0101(03)00229-0

Cited by 21 publications

(13 citation statements)

References 18 publications

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“…The group perfused only with the venom showed a moderate deposit of a proteinaceous material in the tubules and urinary space (8). The group perfused only with the antivenom presented small amount of a proteinaceous material in the renal tubules, similar to the control group.…”

Section: Results Of Histological Alterations In Kidneysupporting

confidence: 60%

“…Some studies demonstrated that the action of the venom on endothelium may contribute to ischemia (7). On isolated rat kidney we observed that T. nattereri venom causes changes in renal function including increase in: perfusion pressure, renal vascular resistance, urinary flow and glomerular filtration rate (8). Local acute inflammatory response induced by the venom was also registered (9).…”

Section: Conflicts Of Interestmentioning

confidence: 78%

“…These alterations were probably due to myotoxic agents or to a direct effect of the venom on renal cells causing the release of vasoactive factors (8).…”

Section: Discussionmentioning

confidence: 99%

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Antivenom action on renal effects induced by Thalassophryne nattereri venom

Martins¹,

Barbosa²,

Sousa³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:Thalassophryne nattereri (niquim) is a venomous fish responsible for numerous accidents involving fishermen in northern and northeastern Brazil. The aim of the present investigation was to evaluate the action of antivenom on renal effects caused by Thalassophryne nattereri venom. Isolated kidneys of Wistar rats were perfused with a previously dialyzed Krebs-Henseleit solution containing 6 g% bovine serum albumin. The antivenom action was studied through perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF) and glomerular filtration rate (GFR). The niquim venom (1 µg/mL), the antivenom alone (1 µg/mL) or the venom incubated with antivenom were added to the system 30 minutes after the beginning of each perfusion. Previous works have shown venom induced-alterations of renal function parameters. In the isolated rat kidney, T. nattereri venom (1 µg/mL) increased the perfusion pressure and renal vascular resistance at 60, 90 and 120 minutes. UF and GFR also increased at 60, 90 and 120 minutes when compared with the control group; however, no effects were observed on the percent of sodium (%TNa

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Section: Results Of Histological Alterations In Kidneysupporting

confidence: 60%

Section: Conflicts Of Interestmentioning

confidence: 78%

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Antivenom action on renal effects induced by Thalassophryne nattereri venom

Martins¹,

Barbosa²,

Sousa³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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“…The effects of S. argus venom on the kidney are very well confirmed by the histopathological changes observed in the kidney of the Pterois volitans venom-treated rats (Balasubhashini et al 2006). Studies on the histological evaluation of rat kidney perfused with Thallasophryne nattereri venom showed moderate deposits of proteinaceous material in the renal tubule (Faco et al 2003).…”

Section: Discussionmentioning

confidence: 94%

Renal Lysosomal Functions on Exposure toScatophagus argusVenom in Experimental Mice

Sivan

Radhakrishnan

2007

Toxicology Mechanisms and Methods

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Scatophagus argus venom was extracted by homogenization and centrifugation. The lysosome-enriched fraction of mice kidney was isolated by differential centrifugation. To study the effect of S. argus venom on lysosomal membrane stability, acid phosphatase and cathepsin D, the two marker enzymes for lysosome, were assayed. In the renal lysosomal enriched fraction incubated with different concentrations of venom and at various time intervals, in vivo and in vitro studies were done. Studies reveal that with increasing concentration of venom and time, the activity of the enzymes increased, which showed that the membrane integrities decreased for both in vitro and in vivo studies. The activity of the enzymes acid phosphatase and cathepsin D showed a dose-dependent relationship with S. argus venom.

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“…O seu veneno ou suas toxinas vêm sendo amplamente estudados quanto a diferentes aspectos como: os efeitos tóxicos causados nas vítimas humanas (FONSECA e LOPES-FERREIRA, 2000) e em modelos experimentais (LOPES-FERREIRA et al, 1998, 2001, 2002FACÓ et al, 2003), os aspectos bioquímicos e farmacológicos (LIMA et al, 2003;LOPES-FERREIRA et al, 2004;MAGALHÃES et al, 2005MAGALHÃES et al, , 2006, a capacidade de indução de anticorpos neutralizantes para os principais efeitos tóxicos em camundongos e os mecanismos imunológicos envolvidos nesta resposta GRUND et al, 2006;PIRAN-SOARES et al, 2007). Assim, o estudo deste veneno tornou-se importante para o entendimento do processo de lesão e regeneração tecidual visto no envenenamento como para o entendimento da relação entre as diferentes classes de toxinas contidas nele e os mecanismos de ação.…”

Section: Discussionunclassified

Avaliação do papel da nattectina, toxina do veneno de Thalassophryne nattereri, na respota imune inata e específica.

Saraiva¹

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RESUMOO objetivo deste trabalho foi avaliar a resposta imune inata induzida pela Nattectina, uma lectina tipo C isolada do veneno de Thalassophryne nattereri, em um modelo de inflamação murina e subseqüentemente seu papel na ativação das células dendríticas e indução da resposta imune específica. Nossos resultados mostram que a Nattectina induziu uma reação inflamatória na cavidade peritoneal de camundongos BALB/c caracterizada pelo influxo predominante de neutrófilos em 1 e 6 horas, substituído por macrófagos em 24 horas. Análises do sobrenadante da suspensão celular da cavidade peritoneal mostraram que a Nattectina induziu a liberação de mediadores inflamatórios como LTB 4 e PGE 2 em 6 horas, IL-1β em 1 e 6 h, IL-6 em 1 h, MCP-1 em 1 e 24 h e KC em 1 h. Além disso, IL-12p70 foi observada no peritônio em 1 e 24 h e IL-10 em 6 e 24 h. Células dendríticas imaturas de origem mieloíde (CD11b high CD11c high B220 low ) foram amadurecidas pela Nattectina com aumento na expressão das moléculas MHC de classe II e das coestimulatórias CD40, CD80 e CD86 e expressão de metaloproteinases de matriz MMP-2 e MMP-9, com síntese de IL-10 e IL-12p70. As células dendríticas imaturas apresentaram grande capacidade de captura e apresentação da Nattectina. Como conseqüência da ativação das células dendríticas, a Nattectina foi capaz de desencadear uma reposta imune adaptativa caracterizada pela ativação preferencial de linfócitos Th1, com síntese de IFN-γe de IL-10, favorecendo a produção de IgG2a específica. A presença de IL-10 pode ser responsável pela limitação da inflamação patológica induzida pelo veneno de T. nattereri e pela manutenção da inflamação crônica não resolvida observada no envenenamento. Finalmente, a Nattectina é um potente candidato antigênico juntamente com as células dendríticas para uso em imunoterapias.PDF Creator: PDF4U Pro DEMO Version. If you want to remove this line, please purchase the full version ABSTRACTRecently, it was identified a C-type lectin in the venom of Thalassophryne nattereri fish named Nattectin. In this study, we investigated the innate and adquired immune responses induced by Nattectin and consequently its role in the dendritic cell activation. The toxin was i.p. injected in BALB/c mice for evaluation of cellular recruitment and inflammatory mediators, antibody and cytokine production. Dendritic cells obtained after bone marrow cell culture with GM-CSF for 7d were stimulated with Nattectin for 24h. The expression of surface molecules and MMPs were analyzed by FACS and immunohistochemistry, respectively. Nattectin induced a significant cellular recruitment into peritoneal cavity, mainly neutrophils at 1 and 6hfollowed by macrophages at 24h. One hour after the Nattectin injection the levels of IL-6, IL-1β, MCP-1 and KC were detected, however in 6 or 24h only IL-1β and IL-10 or MCP-1 and IL-12p70 were determined, respectively. Nattectin induced the PGE 2 and LTB 4 synthesis at 6h. After immunization, mice produced elevated levels of anti- immune response that might be related to its activit...

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Effects of Thalassophryne nattereri fish venom in isolated perfused rat kidney

Cited by 21 publications

References 18 publications

Antivenom action on renal effects induced by Thalassophryne nattereri venom

Antivenom action on renal effects induced by Thalassophryne nattereri venom

Renal Lysosomal Functions on Exposure toScatophagus argusVenom in Experimental Mice

Avaliação do papel da nattectina, toxina do veneno de Thalassophryne nattereri, na respota imune inata e específica.

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