Effects of Tetrandrine on Apoptosis and Radiosensitivity of Nasopharyngeal Carcinoma Cell Line CNE

Abstract

Tetrandrine is known to exert antitumor effect, however, little is known about its effect on nasopharyngeal carcinoma cells. In this study, we tested tetrandrine-induced apoptosis and radiosensitivity in nasopharyngeal carcinoma cell line CNE and investigated the possible mechanisms. Using flow cytometry and DNA electrophoresis, we found that tetrandrine could induce cell apoptosis. Further, it was shown that the level of Bcl-2 mRNA decreased and Bax mRNA increased after addition of tetrandrine by using reverse transcription-polymerase chain reaction. X-ray-induced G2 arrest was abrogated by treatment with tetrandrine, as detected by flow cytometry and mitotic index. The accumulation of cyclinB1 protein and the suppression of Cdc2 tyrosine-15 and Cdc25C serine-216 phosphorylation were detected in irradiated cells treated with tetrandrine using Western blot analysis. Taken together, these results show that tetrandrine can induce apoptosis and abrogate radiation-induced G2 arrest in CNE cells.

XPA A23G polymorphism is associated with the elevated response to platinum-based chemotherapy in advanced non-small cell lung cancer

“…A common single-nucleotide polymorphism (A23G) in the 5 0 non-coding region (at position 24 from the ATG start codon) of the gene was identified [18] and its functional relevance remains to be determined, although a decreased risk for lung cancer has been mentioned to be associated with this polymorphism [20][21][22]. This polymorphism is located in the vicinity of the translation initiation codon, and hence may alter translation efficiency.…”

Tetrandrine Induces Apoptosis and Triggers Caspase Cascade in Human Bladder Cancer Cells

“…It has been clinically used as an anti-rheumatic, anti-inflammation, and anti-hypertensive agent in traditional Chinese medicine [5]. Accumulating evidence indicates that tetrandrine possesses an anticancer effect against various tumors, including leukemia [6], hepatocellular carcinoma [7][8][9], colon cancer [8,10], lung carcinoma [11,12], glioma [13], and nasopharyngeal carcinoma [14]. The inhibitory effects of tetrandrine against various cancer cells have been reported to be associated with growth inhibition, anti-inflammation, cell cycle regulation, apoptosis induction, chemosensitization, inhibition of angiogenesis, and reversal of multi-drug resistance [5].…”

Silver nanocrystals sensitize magnetic-nanoparticle-mediated thermo-induced killing of cancer cells

“…Annexin V-FITC and Propidium Iodide staining An in situ apoptosis detection kit was used for Annexin V-FITC binding and PI staining (KeyGEN) [23]. After incubation with AgNPs, heat, and AgNPsþ heat for 24 h, 2 Â 10 5 cells from each group were harvested and washed with PBS, and then resuspended in binding buffer (10 mM HEPES, 140 mM sodium chloride, 1.8 mM calcium chloride, pH 7.4).…”