Effects of Tetrachloroethylene on the Viability and Development of Embryos of the Japanese Medaka, Oryzias latipes

Spencer, Hattie; Hussein, Wedad R.; Tchounwou, Paul B.

doi:10.1007/s00244-001-0050-1

Cited by 11 publications

(3 citation statements)

References 11 publications

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“…In the embryo-larval development, the effects of xenobiotics on hatching rate is due to the exertion of toxicity response which disturbs enzymatic pathways in morphological and physiological processes at this vital stage of their life cycle. The delay in the hatching rate of the diuron-exposed embryos in this study was in line with Spencer et al [54], which reported that exposure of Japanese medaka (Oryzias latipes) to tetrachloroethylene (PCE), a potential EDC delayed hatching. Kao et al [55] demonstrated that the survival rate and hatching rate of Danio rerio embryos were considerably unaffected after exposure to diuron in comparison to the control group.…”

Section: Discussionsupporting

confidence: 92%

Effect of Diuron on Embryo-Larval Development of Javanese Medaka (Oryzias javanicus, Bleeker 1854)

Ibrahim¹,

Zulkifli²,

Azmai³

et al. 2020

Preprint

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Some herbicides exert hormetic or biphasic non-monotonic dose-response (NMDR), which is one of the major challenges for ecological risk assessment (ERA) of pesticides pollution. In this study, fish embryo toxicity test (FET) with Javanese medaka (Oryzias javanicus) to sublethal concentration of diuron was determined. Inverted U-shape heart rate was observed at 3 days post-exposure (dpe) and 7 dpe. However, at 13 dpe the heart rate (104 ± 2.90 heartbeat/min.) decreased in 10.00 mg.L-1 exposed-embryos. At 20 dpe, hatchability and survival rate were reduced in 5.00 mg.L-1 and 10.00 mg.L-1 exposed groups. Hormetic developmental deformities were observed in embryo-larvae of Javanese medaka. The results revealed a biphasic effect of low concentrations of diuron on some morphological and physiological features of Javanese medaka embryo-larvae, which might be attributed to endocrine disruption of this herbicide. Further studies to support these effects were recommended.

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Section: Discussionsupporting

confidence: 92%

Effect of Diuron on Embryo-Larval Development of Javanese Medaka (Oryzias javanicus, Bleeker 1854)

Ibrahim¹,

Zulkifli²,

Azmai³

et al. 2020

Preprint

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“…For example, exposure to atrazine and ametrine herbicides resulted in increased MN [55]; in contrast, a different herbicide, pendimethalin, showed increased ENA but not MN [56]. Tetrachloroethene, the only CEC analyzed that presented higher concentrations in P2 than P1, is a dry-cleaning compound widely used in the textile industry, known to have toxic effects in fish [57,58]. Although evidence in humans supports the link between the MN formation and this compound [59], to the authors’ knowledge, there are no published studies demonstrating this association in fish.…”

Section: Discussionmentioning

confidence: 99%

Towards Non-Invasive Methods in Measuring Fish Welfare: The Measurement of Cortisol Concentrations in Fish Skin Mucus as a Biomarker of Habitat Quality

Carbajal

Soler

Talló-Parra

et al. 2019

Animals

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Simple SummaryThe analysis of circulating cortisol has been by far the most common method used as a means to assess fish stress responses and, thus, animal welfare. To avoid many of the drawbacks inherent to blood sampling, cortisol can be less-invasively detected in fish skin mucus. The measurement of cortisol in skin mucus however, has, to date, only been demonstrated as suitable for farm fish, although its application to free-ranging animals would offer many advantages. The present study was therefore designed to evaluate the applicability of skin mucus cortisol analysis as a potential tool to assess habitat quality. To that end, wild fish residing in environments of different habitat quality were sampled for blood and skin mucus. First, several physiological endpoints typically used as indicators of exposure to pollutants were accurately related to the habitat quality in the Catalan chub (Squalius laietanus). Second, cortisol levels in blood were also compared between habitats, and they were successfully correlated to skin mucus cortisol concentrations. Finally, we contrasted the patterns of response of all the endpoints assessed to skin mucus cortisol levels across the sites. The strong linkages detected in this study provide new evidence that the measurement of cortisol in skin mucus could be potentially used as a biomarker of habitat quality in freshwater fish. AbstractCortisol levels in fish skin mucus have shown to be good stress indicators in farm fish exposed to different stressors. Its applicability in free-ranging animals subject to long-term environmental stressors though remains to be explored. The present study was therefore designed to examine whether skin mucus cortisol levels from a wild freshwater fish (Catalan chub, Squalius laietanus) are affected by the habitat quality. Several well-established hematological parameters and cortisol concentrations were measured in blood and compared to variations in skin mucus cortisol values across three habitats with different pollution gradient. Fluctuations of cortisol in skin mucus varied across the streams of differing habitat quality, following a similar pattern of response to that detected by the assessment of cortisol levels in blood and the hematological parameters. Furthermore, there was a close relationship between cortisol concentrations in skin mucus and several of the erythrocytic alterations and the relative proportion of neutrophils to lymphocytes. Taken together, results of this study provide the first evidence that skin mucus cortisol levels could be influenced by habitat quality. Although results should be interpreted with caution, because a small sample size was collected in one studied habitat, the measurement of cortisol in skin mucus could be potentially used as a biomarker in freshwater fish.

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“…In continuation of our studies on the effects of environmental pollutants on Japanese medaka fish 18–19 , a small laboratory fish, considered as complementary to zebrafish 20 , we studied the potential toxic effects of OXY, an herbicide, widely used in agriculture. This compound is almost insoluble in water (0.116 mg/L at 25 °C); to improve the solubility of OXY in ERM or BSS, we used 0.1% acetone as a solvent and parallel control embryos/larvae were maintained in 0.1% acetone-containing media as vehicle control in addition to the normal control maintained in ERM.…”

Section: Discussionmentioning

confidence: 99%

Toxicity implications for early life stage Japanese medaka (Oryzias latipes) exposed to oxyfluorfen

Powe

Dasmahapatra

Russell

2018

Environmental Toxicology

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We investigated the potential toxic effects of Oxyfluorfen (OXY), an herbicide used in agriculture, on the embryo-larval development of Japanese medaka fish (Oryzias latipes). Embryos (1-day postfertilization) and larvae (2-day posthatch) were exposed to OXY (0.5-8 mg/L) for 96 h and evaluated for mortality and hatching on embryos, and the mortality and growth on larvae during depuration. It was observed that the embryo-mortality was inconsistently altered by OXY; only the 2 mg/L group showed significant reduction on embryo survivability. However, larval-mortality was concentration-dependent and OXY exposure induced scoliosis-like phenotypic features in the surviving larvae; the calculated LC was 5.238 mg/L. Our data further indicated that larval skeleton, both axial and appendicular, was the potential target site of OXY. Skeletal growth in larvae exposed to 2 mg/L was inhibited significantly until 1 week of depuration with regard to the linear lengths of neurocranium, Meckel's cartilage, caudal vertebrae (first 10) in the axial skeletons, and pectoral fin and urostyle in the appendicular skeletons. Moreover, the total protein content remained unaltered by OXY after 96 h exposure; while the RNA concentration was reduced significantly in larvae exposed to 2 mg/L. Expression analysis of several genes by quantitative real-time RT-PCR (RT-qPCR) showed significant upregulation of zic5, a zinc-finger type transcription regulator, at the transcription level. This study indicated that the scoliosis induced by OXY in Japanese medaka larvae was the result of stunted skeletal growth, probably because of deregulation of zinc-finger type transcription regulators, at the genomic level.

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Effects of Tetrachloroethylene on the Viability and Development of Embryos of the Japanese Medaka, Oryzias latipes

Cited by 11 publications

References 11 publications

Effect of Diuron on Embryo-Larval Development of Javanese Medaka (<em>Oryzias</em> <em>javanicus</em>, Bleeker 1854)

Effect of Diuron on Embryo-Larval Development of Javanese Medaka (<em>Oryzias</em> <em>javanicus</em>, Bleeker 1854)

Towards Non-Invasive Methods in Measuring Fish Welfare: The Measurement of Cortisol Concentrations in Fish Skin Mucus as a Biomarker of Habitat Quality

Toxicity implications for early life stage Japanese medaka (Oryzias latipes) exposed to oxyfluorfen

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