Effects of Temporary and Permanent Muscle Denervation on Fat Graft Retention in the Latissimus Dorsi Muscle: An Experimental Study in Rats

Otani, Naoya; Tomita, Koichi; Kuroda, Kagayaki; Taminato, Mifue; Kubo, Tateki

doi:10.1007/s00266-022-02813-x

Cited by 4 publications

(3 citation statements)

References 35 publications

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“…As previously described, 46 H&E-stained sections were evaluated for histologic measures, including fat cell integrity, cysts, and vacuoles, inflammation, and fibrosis, using the following 6 grades: 0 (absent), 1 (minimal), 2 (minimal to moderate), 3 (moderate), 4 (moderate to extensive), and 5 (extensive).…”

Section: Methodsmentioning

confidence: 99%

“…For each immunostained section, the area occupied by perilipin-positive viable adipocytes and the number of CD31-positive capillaries were measured as previously described. 46…”

Section: Methodsmentioning

confidence: 99%

“…For each immunostained section, the area occupied by perilipin-positive viable adipocytes and the number of CD31-positive capillaries were measured as previously described. 46 TUNEL staining was performed to detect cells undergoing apoptosis using the CF488A TUNEL assay apoptosis detection kit (Biotium, Inc.) according to the manufacturer's instructions. For quantitative analysis, the percentage of TUNEL-positive cells among the total number of DAPI-positive nuclei were measured as previously described.…”

Section: Histologic Analysismentioning

confidence: 99%

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“Hydrogen-generating Si-based agent improves fat graft survival in rats”

Otani

Tomita

Kobayashi

et al. 2023

Plastic &Amp; Reconstructive Surgery

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Background: Regulating excessive inflammation and oxidative stress in fat grafting may improve retention rates. Hydrogen effectively combats oxidative stress and inflammation and reportedly inhibits ischemia-reperfusion injury in various organs. Unfortunately, with conventional methods of hydrogen administration, incorporating hydrogen continuously into the body over a long period of time is difficult. We hypothesized that a Silicon (Si)-based agent we recently developed would aid in fat grafting as it can generate large amounts of hydrogen continuously in the body. Methods: Fat grafting was performed on the backs of rats fed either a normal or 1.0 wt% Si-based agent-containing diet. To investigate synergistic effects with adipose-derived stromal cells (ASCs), which improve retention rates of fat grafting, fat grafting with ASCs (1.0×10 5/400 mg fat) was also performed in each rat. Postoperative retention rates of grafted fat over time, inflammatory indices, apoptosis and oxidative stress markers, histological findings, and expression levels of inflammation-related cytokines and growth factors were compared between the four groups. Results: Intake of Si-based agent and addition of ASCs significantly reduced inflammatory indices, oxidative stress, and apoptosis of grafted fat, and improved long-term retention rates, histological parameters, and grafted fat quality. Under our experimental conditions, intake of the Si-based agent and addition of ASCs yielded comparable improvements in fat graft retention. Combining the two enhanced these effects even further. Conclusion: Oral administration of the hydrogen-generating Si-based agent may improve grafted fat retention by regulating the inflammatory response and oxidative stress in grafted fat. Clinical Relevance Statement: This study demonstrates improved grafted fat retention rates using a Si-based agent. This Si-based agent has the potential to expand the range of therapeutic indications of hydrogen-based therapy to conditions for which hydrogen has yet to be found effective, such as fat grafting.

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Section: Methodsmentioning

confidence: 99%

“…For each immunostained section, the area occupied by perilipin-positive viable adipocytes and the number of CD31-positive capillaries were measured as previously described. 46…”

Section: Methodsmentioning

confidence: 99%

Section: Histologic Analysismentioning

confidence: 99%

See 1 more Smart Citation

“Hydrogen-generating Si-based agent improves fat graft survival in rats”

Otani

Tomita

Kobayashi

et al. 2023

Plastic &Amp; Reconstructive Surgery

Self Cite

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Investigation of the Effect of Mobile and Immobile Regions on Fat Graft Viability: An Experimental Study in a New Model

Çakır Bozkurt,

Yoleri,

Temiz

2024

Aesth Plast Surg

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Background Fat grafts are widely used in plastic, aesthetic and reconstructive surgery. Their unpredictable resorption is their main disadvantage. A review of the literature shows that there is a lack of research on the effect of mobile and immobile regions on fat graft survival in fat graft applications. Objective Our aim was to investigate the relationship of fat graft survival with mobile and immobile region in a new experimental model. Methods Twenty-four male Wistar albino rats were randomly divided into two groups (n=12). Fat grafts were harvested from the right inguinal region of the rat. In Group 1, the fat graft was placed in the subcutaneous pouch formed in the scalp region of the rat. In Group 2, fat grafts were placed in the pouch formed in the posterior cervical region of the rat. At the end of 6 weeks, the weights and histopathology of the fat grafts were evaluated. Histopathological examinations were performed in a blinded fashion. Results The weights of the fat grafts were found to be higher in Group 1. At the same time, histopathological examinations showed that vascular density was higher in Group 1. There was no statistically significant difference in other histopathological examinations. Conclusion The mobile and immobile areas may have different effects on the survival of transplanted fat grafts. Sliding movement between muscle and skin in the mobile zone puts stress on the fat graft. In our study, the mobile site was shown to have a negative effect on the vascularity of the fat graft. It was observed that the vascular density was higher in the fat graft placed in the immobilised area. Further studies on the increase in vascularity can be carried out using the new experimental model we have created. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

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