Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor

Sheng, Jie; Kim, Hyun-Woo; Badalamenti, Jonathan P.; Zhou, Chao; Sridharakrishnan, Swathi; Krajmalnik‐Brown, Rosa; Rittmann, Bruce E.; Vannela, Raveender

doi:10.1016/j.biortech.2011.09.083

Cited by 63 publications

(34 citation statements)

References 29 publications

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“…It was reported that the lipid fluidity of the cell membrane decreased as temperature decreased. The lipid production rate also decreased after the temperature dropped to 22 and 18 o C [22], and the total carbohydrate content appeared to increase below 28 o C [23]. Based on these results, the Synechocystis cells were exposed to the stress conditions for 30 min and were collected for proteome analysis.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of Synechocystis sp. PCC6803 responses to low-temperature and high light conditions

Hong

Kim

Jang

et al. 2014

Biotechnol Bioproc E

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The global changes in protein expression of Synechocystis sp. PCC6803, a photosynthetic bacterium for the production of secondary metabolites as a green cell factory, were investigated by proteome separation and a subsequent tandem mass spectrometry. Two different proteome separation techniques, strong cation exchange chromatography and off-gel electrophoresis, were applied. The combination of the two proteome separation techniques enabled the comparative analysis of the differential regulation of the Synechocystis proteome in response to two different environmental factors, temperature and light. A total of 1,483 proteins were identified, which represent over 40% of the genes in Synechocystis. Our data showed that fatty acid metabolism was inhibited by (3R)-hydroxymyristol acyl carrier protein dehydrase (Sll1605) under low temperature conditions. The expression of UDP-N-acetylglucosamine acyltransferase (Sll0379) and 3-O- [3-hydroxymyristoyl] glucosamine N-acyltransferase (Slr0776), which is involved in lipopolysaccharide metabolism, was not observed under high light conditions. Under high light exposure, proteins related to iron-sulfur metabolism were detected, which may be responsible for maintaining the redox potential of the photosystem. High light under low temperature caused severe damage to the photosystem. Some of the responses to these stresses were similar to those previously reported for other photosynthetic organisms. Notably, this study revealed the followings: (i) low temperature inhibits fatty acid synthesis; (ii) high light inhibits lipopolysaccharides synthesis and stimulates the expression of iron-sulfur related proteins; and (iii) high light under low temperature induces the photorespiratory cycle. The global proteomic analysis clearly showed that stress conditions such as low temperature and/or high light induce cellular metabolisms related with the protection of their photosystems in the model microalga Synechocystis sp. PCC6803.

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Section: Resultsmentioning

confidence: 99%

Proteomic analysis of Synechocystis sp. PCC6803 responses to low-temperature and high light conditions

Hong

Kim

Jang

et al. 2014

Biotechnol Bioproc E

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“…Environmental growth conditions determine the fatty acid profile of lipid in microalgae and cyanobacteria [8,[15][16][17][18][19][20]. Quintana et al [9] reported that growth conditions affected the fatty acid composition of different cyanobacteria species, Liu et al [21] noted that the degree of unsaturation in fatty acids increased at lower temperatures, Walsh et al [22] found that the amount of polyunsaturated fatty acids (PUFAs) decreased in favor of monounsaturated fatty acids at high light intensities, and Gombos et al [23] found that temperature had a strong impact on the degree of unsaturation of fatty acid groups.…”

Section: Introductionmentioning

confidence: 99%

Effects of light intensity and carbon dioxide on lipids and fatty acids produced by Synechocystis sp. PCC6803 during continuous flow

et al. 2015

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We studied the effects of light intensity (LI) and CO 2 supply on pH and total lipid production and fatty acids by Synechocystis sp. PCC6803 during continuous-flow operation of a photobioreactor having continuous nutrient supply. The temperature was fixed at 30°C, and the LI pattern mimicked a day/night light cycle from 0 to 1920 μmol/m 2 s. The CO 2 supply varied from 1 to 5% v/v of total air. The total lipid content increased proportionally to LI, reaching a high content of 14% of dry weight (DW) at the highest LI at 3% CO 2 . In contrast, LI had no significant influence on the total fatty acid content, which was 3.4% ± 0.5% DW, measured as fatty acid methyl esters (FAMEs). Palmitic acid (C16:0) was the main fatty acid (52% of FAMEs), but γ-linolenic acid (C18:3 n6 ) and linoleic acid (C18:2) were significant at 20% and 14% of total FAMEs, respectively. Also, α-linolenic acid (C18:3n3), oleic acid (C18:1), and palmitoleic acid (C16:1) represented 5%, 4%, and 4% of the total FAMEs, respectively. In case of C16:0, its highest content was achieved at LI of 400 to 1500 μmol/m 2 s and pH media values from 7.2 to 8.8 (3% CO 2 ). The highest formation of C16:1 and C18:1 (desirable for biodiesel production) occurred with LI up to 600 μmol/m 2 s at pH 9 (3% CO 2 ). Stearic acid (C18:0) and linoleic acid (C18:2) contents did not vary with LI or pH, but α-linolenic acid (C18:3 n3 ) formation occurred with patterns opposite to C18:3 n6 , C16:0, and C16:1. LI of 400 to 1600 μmol/m 2 s and pH range from 7.7 to 8.7 led to the highest values of C18:3 n6 (0.8% DW), but C18:3 n3 was suppressed by these conditions, supporting a desaturation pathway in Synechocystis. These results point to strategies to optimize LI, CO 2 , and pH, to enhance the fatty acid production profile for biofuel production.

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“…While effective, lipid extraction with chloroform and methanol is infeasible for large-scale application, because these solvents are hazardous materials and expensive (Zbinden et al, 2013). Moreover, these strong 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 solvents co-extract non-lipid components from the biomass, necessitating extensive downstream refining of the valuable fuel precursors (Sheng et al, 2011a).…”

Section: Introductionmentioning

confidence: 99%

Effects of pulsed electric field treatment on enhancing lipid recovery from the microalga, Scenedesmus

Lai

Parameswaran

et al. 2014

Bioresource Technology

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Chloroform and methanol are superior solvents for lipid extraction from photosynthetic microorganisms, because they can overcome the resistance offered by the cell walls and membranes, but they are too toxic and expensive to use for large-scale fuel production. Biomass from the photosynthetic microalga Scenedesmus, subjected to a commercially available pre-treatment technology called Focused-Pulsed® (FP), yielded 3.1-fold more crude lipid and fatty acid methyl ester (FAME) after extraction with a range of solvents. FP treatment increased the FAME-to-crude-lipid ratio for all solvents, which means that the extraction of non-lipid materials was minimized, while the FAME profile itself was unchanged compared to the control. FP treatment also made it possible to use only a small proportion of chloroform and methanol, along with isopropanol, to obtain equivalent yields of lipid and FAME as with 100% chloroform plus methanol. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 AbstractChloroform and methanol are superior solvents for lipid extraction from photosynthetic microorganisms, because they can overcome the resistance offered by the cell walls and membranes, but they are too toxic and expensive to use for largescale fuel production. Biomass from the photosynthetic microalga Scenedesmus, subjected to a commercially available pre-treatment technology called FocusedPulsed® (FP), yielded 3.1-fold more crude lipid and fatty acid methyl ester (FAME) after extraction with a range of solvents. FP treatment increased the FAME-to-crudelipid ratio for all solvents, which means that the extraction of non-lipid materials was minimized, while the FAME profile itself was unchanged compared to the control. FP treatment also made it possible to use only a small proportion of chloroform and methanol, along with isopropanol, to obtain equivalent yields of lipid and FAME as with 100% chloroform plus methanol.

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Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor

Cited by 63 publications

References 29 publications

Proteomic analysis of Synechocystis sp. PCC6803 responses to low-temperature and high light conditions

Proteomic analysis of Synechocystis sp. PCC6803 responses to low-temperature and high light conditions

Effects of light intensity and carbon dioxide on lipids and fatty acids produced by Synechocystis sp. PCC6803 during continuous flow

Effects of pulsed electric field treatment on enhancing lipid recovery from the microalga, Scenedesmus

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