Effects of temperature, salinity and their interaction on growth of Japanese Gambierdiscus spp. (Dinophyceae)

Yoshimatsu, Takamichi; Yamaguchi, Haruo; Iwamoto, Haruka; Nishimura, Tomohiro; Adachi, Masao

doi:10.1016/j.hal.2014.03.007

Cited by 38 publications

(35 citation statements)

References 36 publications

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“…G. balechii had a similar growth rate as G. excentricus but a substantially smaller cellular biovolume. The overall low growth rates (< 0.5 divisions day -1 ) observed in this study are consistent with those previously reported in the literature (Kibler et al, 2012; submitted to PLoS One on Feb 2017; Xu et al, 2016;Yoshimatsu et al, 2014). If a comparison could be made, it can be concluded that, in the present study, Gambierdiscus strains behaved as slow-growers when cultured under CCHFR and IFREMER laboratory conditions, with µ max < 0.25 divisions day -1 , which appeared similarly low or somewhat lower than those reported in other studies.…”

Section: Maximum Growth Rates and Cellular Biovolumessupporting

confidence: 92%

Toxicity screening of 13 Gambierdiscus strains using neuro-2a and erythrocyte lysis bioassays

et al. 2017

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Species in the epi-benthic dinoflagellate genus Gambierdiscus produce ciguatoxins (CTXs) and maitotoxins (MTXs), which are among the most potent marine toxins known. Consumption of fish contaminated with sufficient quantities of CTXs causes Ciguatera Fish Poisoning (CFP), the largest cause of non-bacterial food poisoning worldwide. Maitotoxins, which can be found in the digestive system of fish, could also contribute to CFP if such tissues are consumed. Recently, an increasing number of Gambierdiscus species have been identified; yet, little is known about the variation in toxicity among Gambierdiscus strains or species. This study is the first assessment of relative CTX- and MTX-toxicity of Gambierdiscus species from areas as widespread as the North-Eastern Atlantic Ocean, Pacific Ocean and the Mediterranean Sea. A total of 13 strains were screened: (i) seven Pacific strains of G. australes, G. balechii, G. caribaeus, G. carpenteri, G. pacificus, G. scabrosus and one strain of an undetermined species (Gambierdiscus sp. Viet Nam), (ii) five strains from the North-Eastern Atlantic Ocean (two G. australes, a single G. excentricus and two G. silvae strains), and (iii) one G. carolinianus strain from the Mediterranean Sea. Cell pellets of Gambierdiscus were extracted with methanol and the crude extracts partitioned into a CTX-containing dichloromethane fraction and a MTX-containing aqueous methanol fraction. CTX-toxicity was estimated using the neuro-2a cytoxicity assay, and MTX-toxicity via a human erythrocyte lysis assay. Different species were grouped into different ratios of CTX- and MTX-toxicity, however, the ratio was not related to the geographical origin of species (Atlantic, Mediterranean, Pacific). All strains showed MTX-toxicity, ranging from 1.5 to 86pg MTX equivalents (eq) cell. All but one of the strains showed relatively low CTX-toxicity ranging from 0.6 to 50 fg CTX3C eq cell. The exception was the highly toxic G. excentricus strain from the Canary Islands, which produced 1426 fg CTX3C eq cell. As was true for CTX, the highest MTX-toxicity was also found in G. excentricus. Thus, the present study confirmed that at least one species from the Atlantic Ocean demonstrates similar toxicity as the most toxic strains from the Pacific, even if the metabolites in fish have so far been shown to be more toxic in the Pacific Ocean.

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Section: Maximum Growth Rates and Cellular Biovolumessupporting

confidence: 92%

Toxicity screening of 13 Gambierdiscus strains using neuro-2a and erythrocyte lysis bioassays

et al. 2017

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“…Gambierdiscus toxicus is known to generally favor warmer conditions, and increased ciguatera fish poisoning has been observed with elevated sea surface temperatures related to El Niño Southern Oscillation (ENSO) and the Pacific Decadal Oscillation (PDO) (Rongo and van Woesik, 2011). But this linkage is correlative, rather than determined, and temperature optima differ substantially among different Gambierdiscus species or phylotypes (Yoshimatsu et al, 2014). The cell-size dependent population response to warming also differs among phytoplankton groups.…”

Section: Anticipated Linkages Between Harmful Algal Blooms and CLImentioning

confidence: 99%

Harmful algal blooms and climate change: Learning from the past and present to forecast the future

et al. 2015

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Climate change pressures will influence marine planktonic systems globally, and it is conceivable that harmful algal blooms may increase in frequency and severity. These pressures will be manifest as alterations in temperature, stratification, light, ocean acidification, precipitation-induced nutrient inputs, and grazing, but absence of fundamental knowledge of the mechanisms driving harmful algal blooms frustrates most hope of forecasting their future prevalence. Summarized here is the consensus of a recent workshop held to address what currently is known and not known about the environmental conditions that favor initiation and maintenance of harmful algal blooms. There is expectation that harmful algal bloom (HAB) geographical domains should expand in some cases, as will seasonal windows of opportunity for harmful algal blooms at higher latitudes. Nonetheless there is only basic information to speculate upon which regions or habitats HAB species may be the most resilient or susceptible. Moreover, current research strategies are not well suited to inform these fundamental linkages. There is a critical absence of tenable hypotheses for how climate pressures mechanistically affect HAB species, and the lack of uniform experimental protocols limits the quantitative cross-investigation comparisons essential to advancement. A HAB “best practices” manual would help foster more uniform research strategies and protocols, and selection of a small target list of model HAB species or isolates for study would greatly promote the accumulation of knowledge. Despite the need to focus on keystone species, more studies need to address strain variability within species, their responses under multifactorial conditions, and the retrospective analyses of long-term plankton and cyst core data; research topics that are departures from the norm. Examples of some fundamental unknowns include how larger and more frequent extreme weather events may break down natural biogeographic barriers, how stratification may enhance or diminish HAB events, how trace nutrients (metals, vitamins) influence cell toxicity, and how grazing pressures may leverage, or mitigate HAB development. There is an absence of high quality time-series data in most regions currently experiencing HAB outbreaks, and little if any data from regions expected to develop HAB events in the future. A subset of observer sites is recommended to help develop stronger linkages among global, national, and regional climate change and HAB observation programs, providing fundamental datasets for investigating global changes in the prevalence of harmful algal blooms. Forecasting changes in HAB patterns over the next few decades will depend critically upon considering harmful algal blooms within the competitive context of plankton communities, and linking these insights to ecosystem, oceanographic and climate models. From a broader perspective, the nexus of HAB science and the social sciences of harmful algal blooms is inadequate and prevents quantitative assessment of impacts of futur...

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“…Cell densities were counted under an optical microscope (IX70; Olympus Corporation, Tokyo, Japan). Growth rate (divisions d 1 ) was calculated by following the method of Yoshimatsu et al (2014). Genomic DNA preparation and RNA-seq analysis Genomic DNA and total RNA were isolated from the algae using a NucleoSpin ® Plant II (MACHEREY-NAGEL GmbH & CO. KG, Düren, Germany) and TriPure Isolation Reagent (Roche Diagnostics GmbH, Mannheim, Germany) following the method reported by Kadono et al (2015), respectively.…”

Section: Algal Culturementioning

confidence: 99%

Expression Profile of Genes Involved in Isoprenoid Biosynthesis in the Marine Diatom <i>Phaeodactylum tricornutum</i>

Kira

Yoshimatsu

Fukunaga

et al. 2016

Environmental Control in Biology

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The marine diatom Phaeodactylum tricornutum is expected to be a source of hydrocarbons and carotenoids that are synthesized via isoprenoid precursor biosynthesis pathways such as the mevalonate (MVA) and the 2-C-methyl-D-erythritol phosphate (MEP) pathways, because the molecular biotechnological techniques for metabolic engineering have been established. In this study, we investigated the expression profiles of the genes, including those in the MVA and MEP pathways, under various culture conditions using RNA-seq analysis to obtain information useful for the metabolic engineering and development of endogenous promoters that can highly drive the expression of transgenes in P. tricornutum. The expression levels of the genes in the MVA pathway, except for the 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) gene, were low under all the conditions tested, and the expression levels of the 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK) and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MDS) genes in the MEP pathway were relatively low. Eleven genes with high expression levels, including the V-type proton ATPase subunit C-like gene, which are potential sources of endogenous promoters in P. tricornutum, were selected. These results are expected to provide useful information for the metabolic engineering of P. tricornutum.

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Effects of temperature, salinity and their interaction on growth of Japanese Gambierdiscus spp. (Dinophyceae)

Cited by 38 publications

References 36 publications

Toxicity screening of 13 Gambierdiscus strains using neuro-2a and erythrocyte lysis bioassays

Toxicity screening of 13 Gambierdiscus strains using neuro-2a and erythrocyte lysis bioassays

Harmful algal blooms and climate change: Learning from the past and present to forecast the future

Expression Profile of Genes Involved in Isoprenoid Biosynthesis in the Marine Diatom <i>Phaeodactylum tricornutum</i>

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