Effects of Temperature, pH and Blood Storage on Osmotic Fragility of Duck Erythrocytes

Oyewale, J. O.; Sanni, A. A.; Ajibade, H. A.

doi:10.1111/j.1439-0442.1991.tb01011.x

Cited by 10 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A maximum of two freeze / thawing cycles was allowed since three or more cycles modify the oxidative status of the blood [20] and storage increases the levels of the markers of oxidative damage [21]. The plasma was assayed for superoxide dismutase activity, catalase activity and malondialdehyde (MDA) level.…”

Section: Blood Collectionmentioning

confidence: 99%

Disease-age Correlated Study of Some Patients with Solitary Hypertension Attending Central Hospital, Benin City, Nigeria

Iyamu¹,

Anionye²,

Onyeneke³

et al. 2012

AJB

View full text Add to dashboard Cite

The study was aimed at verifying how oxidative status varies with age of diabetes mellitus (DM) in sufferers with adequate blood sugar control. Forty-one diabetic otherwise healthy (DOH) volunteers and 57 apparently healthy controls were used and plasma levels of two endogenous antioxidant enzymes (Superoxide dismutase, SOD, and catalase, CAT) as well as two markers of oxidative damage (Malondialdehyde, MDA and erythrocyte osmotic fragility, EOF) were determined. Results obtained showed significant decreases in plasma SOD and CAT activities, and significant increases in plasma levels of MDA and EOF as age of disease increases. In addition, there were clear significant differences between the oxidative status of DOH and apparently healthy control subjects of similar chronological (birth) age group. Plasma MDA and EOF as markers of oxidative damage were significantly higher in DOH subjects compared with healthy control subjects of similar age. However, plasma SOD and CAT activities were observed to be significantly lower in DOH subjects compared with healthy control subject of comparable age. It could thus be concluded that diabetes is associated with progressive increase in tissue oxidative damage.

show abstract

Section: Blood Collectionmentioning

confidence: 99%

Disease-age Correlated Study of Some Patients with Solitary Hypertension Attending Central Hospital, Benin City, Nigeria

Iyamu¹,

Anionye²,

Onyeneke³

et al. 2012

AJB

View full text Add to dashboard Cite

show abstract

“…Haematological parameters in birds have been shown to be influenced by various factors such as age, sex, season and nutrition. Packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count have been reported to increase with age in chickens (Islam et al, 2004), Pigeons (Columba livia forma domestica) (Pavlak et al, 2005), Turkeys (Oyewale &Ajibade, 1990) and Budgerigars (Melopsittacus undulatus) (Harper & Lowe, 1998). Packed cell volume (PCV) and Hb values have also been reported to be higher in males than in females in Turkeys (Oyewale & Ajibade, 1990) and Pigeons (Pavlak et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count have been reported to increase with age in chickens (Islam et al, 2004), Pigeons (Columba livia forma domestica) (Pavlak et al, 2005), Turkeys (Oyewale &Ajibade, 1990) and Budgerigars (Melopsittacus undulatus) (Harper & Lowe, 1998). Packed cell volume (PCV) and Hb values have also been reported to be higher in males than in females in Turkeys (Oyewale & Ajibade, 1990) and Pigeons (Pavlak et al, 2005). Similarly, seasonal fluctuations in PCV and Hb concentrations have been reported in Pigeons in the tropics (Oladele et al, 2001b) and in the cold temperate region (Pavlak et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Evaluations of the diurnal variations in the haematological profiles of domestic fowls

2022

JoSVAS

View full text Add to dashboard Cite

The diurnal fluctuation in the haematological parameters of the domestic fowls was investigated using twenty four (24) male Isa Brown chickens. Twenty four (24) apparently healthy Isa Brown chickens of average mean weight 1.99±0.12 kg (1.85-2.30kg) aged 18 weeks were used for this study. The Isa Brown chickens (24) were randomly assigned to four treatment groups (A to D) of six chickens per group in a completely randomized design (CRD). Group A: Early morning (6am), Group B: Afternoon (12pm), Group C: Evening (6pm) and Group D: Mid Night (12am). Blood samples were collected from the birds at 6:00 am, 12:00 pm, 6:00 pm and 12:00 am, during a 12-hour light and a 12-hour dark period at a six hourly intervals. The mean haemoglobin (Hb) concentration (10.40±0.09g/dl) and packed cell volume (31.00±0.41%) at 12am were significantly (P < 0.05) higher than the other diurnal periods of the day. The mean red blood cell count (RBC) at early morning (6am) was significantly (P < 0.05) higher than the values at 12pm but showed no significant (p > 0.05) difference when compared to the values at 6pm and 12am. The mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) concentrations at 12pm were significantly (P < 0.05) higher when compared to the 6am values. The mean total leucocyte count (TLC), lymphocyte count and monocyte count at 6am were significantly (P < 0.05) higher than the values at 6pm. There was however no significantly (p > 0.05) variations in the mean corpuscular haemoglobin concentrations (MCHC), heterophil count, eosinophil count, basophil count and heterophil/lymphocyte (H/L) ratio among all the diurnal periods investigated. In conclusion, the haemoconcentration produced in this study might be responsible for the higher haematological parameters during the night (12am) and early morning (6am) because the birds were neither feeding nor drinking water at this period of the day

show abstract

“…The osmotic fragility test is most frequently used to evaluate the response of erythrocytes to osmotic pressure [12] and determines their resistance to hypotonicity and hemolysis [13]. Osmotic fragility is affected by extrinsic factors such as pH, temperature, osmolality and blood storage [14][15][16][17] and intrinsic factors such as (i) presence of osmotically active constituents (ii) modifications of the lipids and proteins in the membrane [18]. The osmotic fragility provides a guide to assess the membrane integrity [19,20] and can echo the changes in membrane properties in the erythrocytes [21].…”

Section: Introductionmentioning

confidence: 99%

“…citrate, phosphate, dextrose and adenine; VC 10-Vitamin C as an additive at the concentration of 10mM; VC 30-Vitamin C as an additive at the concentration of 30mM; VC 60-Vitamin C as an additive at the concentration of 60mM; LC 10-L-carnitine as an additive at the concentration of 10mM; LC 30-L-carnitine as an additive at the concentration of 30mM; LC 60-L-carnitine as an additive at the concentration of 60mM; Cur 10-Curcumin as an additive at the concentration of 10mM; Cur 30-Curcumin as an additive at the concentration of 30mM; Cur 60-Curcumin as an additive at the concentration of 60mM. *-VC 60 samples could be stored till day 15 # -LC 30 and LC 60 samples could be stored till day 20 $ -Cur 30 and Cur 60 samples could be stored till day15 …”

mentioning

confidence: 99%

Comparison of the protective nature of antioxidants on stored erythrocytes

Ravikumar¹

2016

Appl Med Res

View full text Add to dashboard Cite

Erythrocytes undergo oxidative damage during storage which overwhelms their antioxidant defense system. The employment of antioxidants as additives during storage could protect erythrocytes against storage induced oxidative stress. This study was designed to evaluate the effects of antioxidants (Vitamin C, L-carnitine and Curcumin) on osmotic stress in erythrocytes of stored blood. Blood was obtained from male Wistar rats (4 months old) during the year 2015 and stored for up to 25 days at 4 ºC in CPDA-1 solution. Samples were divided into 10 groups (n=5) with and without antioxidants (Vitamin C, L-carnitine and Curcumin) at concentrations of 10mM, 30mM and 60mM. Erythrocytes were isolated from stored blood every fifth day and the effects of storage on osmotic fragility and membrane sulfhydryls were studied.

show abstract

Effects of Temperature, pH and Blood Storage on Osmotic Fragility of Duck Erythrocytes

Abstract: Summary The osmotic fragility of duck erythrocytes at different pH and temperatures and during blood storage was studied. The fragility increased in acidic solution and also as the temperature increased. However, storage of blood decreased the fragility of duck erythrocytes.

Cited by 10 publications

References 6 publications

Disease-age Correlated Study of Some Patients with Solitary Hypertension Attending Central Hospital, Benin City, Nigeria

Disease-age Correlated Study of Some Patients with Solitary Hypertension Attending Central Hospital, Benin City, Nigeria

Evaluations of the diurnal variations in the haematological profiles of domestic fowls

Comparison of the protective nature of antioxidants on stored erythrocytes

Contact Info

Product

Resources

About