Effects of Temperature on Mortality of Larval Stable Fly (Diptera: Muscidae) Caused by Five Isolates of Bacillus thuringiensis

Lysyk, Tim; Selinger, L. Brent

doi:10.1603/ec11359

Cited by 14 publications

(12 citation statements)

References 26 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experiments were conducted using Þve B. thuringiensis isolates that had previously shown activity against stable ßy larvae (Lysyk et al 2010, Lysyk andSelinger 2012). Isolates Bacillus thuringiensis thuringiensis HD2 (serotype 1) and Bacillus thuringiensis kurstaki HD945 (serotype 3a3b3c) were originally obtained from the USDAÐARS Culture Collection (National Center for Agricultural Utilization Research, Peoria, IL).…”

Section: Methodsmentioning

confidence: 99%

“…Isolates Bacillus thuringiensis tolworthi 4L3 (serotype 9), Bacillus thuringiensis darmstadiensis 4M1 (serotype 10a10b), and Bacillus thuringiensis thompsoni 4O1 (serotype 12) were originally obtained from the Bacillus Genetic Stock Center (The Ohio State University, Columbus, OH). All isolates were individually cultured as described previously (Lysyk et al 2010, Lysyk andSelinger 2012) in nutrient broth supplemented with MgSO 4 , CaCl 2 , MnCl 2 , and FeSO 4 . Protein content of the cultures was determined using the DC protein assay (Bio-Rad Laboratories, Hercules CA) with bovine serum albumen as the protein standard (Crespo et al 2008).…”

Section: Methodsmentioning

confidence: 99%

“…Several reports have indicated activity against tick species as well (Zhioua et al 1999, Fernández-Ruvalcaba et al 2010. Recently, we have reported on several isolates that have activity against immature stable ßy (Lysyk et al 2010, Lysyk andSelinger 2012). Because these isolates have already shown activity against immature stable ßies, we conducted the current study to examine whether they had activity against adult stable ßy.…”

mentioning

confidence: 97%

See 2 more Smart Citations

Mortality of Adult Stomoxys calcitrans Fed Isolates of Bacillus thuringiensis

Lysyk¹,

Kalischuk-Tymensen

Selinger

2012

jnl. econ. entom.

Self Cite

View full text Add to dashboard Cite

We examined the ability of five isolates of Bacillus thuringiensis Berliner to cause mortality in adult stable flies, Stomoxys calcitrans (L.). Isolates Bacillus thuringiensis tolworthi 4L3 (serotype 9), Bacillus thuringiensis darmstadiensis 4M1 (serotype 10a10b), Bacillus thuringiensis thompsoni 401 (serotype 12), Bacillus thuringiensis thuringiensis HD2 (serotype 1), and Bacillus thuringiensis kurstaki HD945 (serotype 3a3b3c) were administered to adult flies in diets containing blood only, sugar only, and both sugar and blood combined. B. t. tolworthi 4L3 had no effect on adult mortality regardless of the feeding substrate. The remaining isolates tended to cause the greatest mortality when administered in blood alone. B. t. thompsoni 401 was the only isolate that consistently caused adult mortality when fed in blood at concentrations ranging from 0.21 to 50.0 microg of protein per ml of blood. This isolate also caused mortality when applied topically. The time to 50% mortality declined with dose and reached a lower asymptote at approximately equal to 1.3 d at an oral dose of 8.75 microg/ml and at a topical dose of 0.14 microg per fly.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 97%

See 1 more Smart Citation

Mortality of Adult Stomoxys calcitrans Fed Isolates of Bacillus thuringiensis

Lysyk¹,

Kalischuk-Tymensen

Selinger

2012

jnl. econ. entom.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Biological control agents have also been considered as an option, emphasizing the use of entomopathogenic fungi (MORAES et al, 2008;ALVES et al, 2012) and bacterial isolates, such as Bacillus thuringiensis (LYSYK & SELINGER, 2012). However, their use is not a short-term option.…”

Section: Challenges and Outlookmentioning

confidence: 99%

Stomoxys calcitrans (Diptera: Muscidae) outbreaks: current situation and future outlook with emphasis on Brazil

Dominghetti

Barros

Soares

et al. 2015

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

The stable fly (Stomoxys calcitrans) has historically been a pest of dairy cattle and feedlots due to the availability of decaying plant matter mixed with animal excrements in such production systems. In the last few decades, stable fly outbreaks have also been reported in pasture-raised beef cattle, usually associated with wastes accumulated from animal feeding during winter, the introduction of large-scale crop operations near cattle ranches, and/or the inadvertent use of organic fertilizers. Population explosions of Stomoxys flies may also have natural causes, affecting not only domestic and/or wild animals but also humans. This article compiles information on stable fly outbreaks in Brazil and abroad and discusses their causes and consequences.Keywords: Stable fly, cattle ectoparasites, bloodsucking flies. ResumoA mosca-dos-estábulos (Stomoxys calcitrans) tem sido historicamente uma praga em gado de leite e gado de corte em confinamento devido à disponibilidade de matéria orgânica vegetal em decomposição, misturada a dejetos animais, nestes sistemas de produção. Nas últimas décadas, surtos de infestação por esta mosca passaram a ocorrer também em rebanhos de corte mantidos extensivamente, geralmente associados ao acúmulo de resíduos de suplementação alimentar durante o inverno, introdução de culturas agrícolas em larga escala no entorno de propriedades pecuárias e/ou uso inadvertido de fertilizantes orgânicos. Eventualmente, explosões populacionais de Stomoxys podem ter causas naturais, afetando animais domésticos e/ou selvagens, além do próprio homem. Este artigo reúne informações sobre surtos da mosca-dos-estábulos no Brasil e em outros países e discute suas causas e consequências.Palavras-chave: Mosca-dos-estábulos, ectoparasitos de bovinos, moscas hematófagas.

show abstract

“…Due to their high efficacy, B. thuringiensis products (Bt products) have held a large market share out of all microbial biopesticides (31,32), which constitutes about 2% of the total market of insecticides (26,31). Numerous strains of B. thuringiensis have been reported to have activity on dipterans (33)(34)(35), including immature stable flies (36,37). Chromobacterium subtsugae is another example of a recent product brought to market for insect control (38)(39)(40).…”

mentioning

confidence: 99%

Isolation and Characterization of Burkholderia rinojensis sp. nov., a Non-Burkholderia cepacia Complex Soil Bacterium with Insecticidal and Miticidal Activities

Córdova-Kreylos

Fernandez

Koivunen

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

Isolate A396, a bacterium isolated from a Japanese soil sample demonstrated strong insecticidal and miticidal activities in laboratory bioassays. The isolate was characterized through biochemical methods, fatty acid methyl ester (FAME) analysis, sequencing of 16S rRNA, multilocus sequence typing and analysis, and DNA-DNA hybridization. FAME analysis matched A396 to Burkholderia cenocepacia, but this result was not confirmed by 16S rRNA or DNA-DNA hybridization. 16S rRNA sequencing indicated closest matches with B. glumae and B. plantarii. DNA-DNA hybridization experiments with B. plantarii, B. glumae, B. multivorans, and B. cenocepacia confirmed the low genetic similarity (11.5 to 37.4%) with known members of the genus. PCR-based screening showed that A396 lacks markers associated with members of the B. cepacia complex. Bioassay results indicated two mechanisms of action: through ingestion and contact. The isolate effectively controlled beet armyworms (Spodoptera exigua; BAW) and two-spotted spider mites (Tetranychus urticae; TSSM). In diet overlay bioassays with BAW, 1% to 4% (vol/vol) dilution of the whole-cell broth caused 97% to 100% mortality 4 days postexposure, and leaf disc treatment bioassays attained 75% ؎ 22% mortality 3 days postexposure. Contact bioassays led to 50% larval mortality, as well as discoloration, stunting, and failure to molt. TSSM mortality reached 93% in treated leaf discs. Activity was maintained in cell-free supernatants and after heat treatment (60°C for 2 h), indicating that a secondary metabolite or excreted thermostable enzyme might be responsible for the activity. Based on these results, we describe the novel species Burkholderia rinojensis, a good candidate for the development of a biocontrol product against insect and mite pests.

show abstract

Effects of Temperature on Mortality of Larval Stable Fly (Diptera: Muscidae) Caused by Five Isolates of Bacillus thuringiensis

Cited by 14 publications

References 26 publications

Mortality of Adult <I>Stomoxys calcitrans</I> Fed Isolates of <I>Bacillus thuringiensis</I>

Mortality of Adult <I>Stomoxys calcitrans</I> Fed Isolates of <I>Bacillus thuringiensis</I>

Stomoxys calcitrans (Diptera: Muscidae) outbreaks: current situation and future outlook with emphasis on Brazil

Isolation and Characterization of Burkholderia rinojensis sp. nov., a Non-Burkholderia cepacia Complex Soil Bacterium with Insecticidal and Miticidal Activities

Contact Info

Product

Resources

About