Effects of Temperature on In Vitro Short‐Term Storage of Sterlet Sturgeon (Acipenser Ruthenus) Ova

Abstract

Artificial propagation of sturgeons is becoming increasingly important for recovery efforts as well as for commercial production. Sterlet Acipenser ruthenus is a common Eurasian sturgeon with a small body size and one of the fastest reproductive cycles among the sturgeons. The practical question being addressed in this study was how long fertilization of ovulated eggs can be delayed without substantially reducing the hatching rate, and an ancillary question is under what' temperature conditions do eggs retain good quality. Broodstock were injected with homogenized carp pituitary extract (CPE); ovulated eggs from three females were allocated to various treatment groups for temperature storage (control, 7°C, 11°C, 15°C and 19°C) until fertilized. Storage times at the regulated temperatures prior to fertilization were for 2.5, 5.0, 7.5 and 10.0 h. After the selected storage times in ovarian fluid, eggs were fertilized and transferred to incubation cages and then they were counted. Three replicates were allocated to each storage period and temperature. Hatched larvae were counted at 7-day post-fertilization. We found that sterlet eggs do not need to be fertilized immediately after collection. Reasonably good quality was retained for several hours if temperature conditions are fairly cool and stable. Eggs retained good quality when stored at 7°C and 11°C for up to 10 h with 54.1 ± 2.9 to 69.9 ± 7.9% hatching success, but egg quality was significantly reduced after 5-h storage at 19°C (p < 0.01) and 7.5-h storage at 15°C (p < 0.05) compared to cooler temperatures. Uniform temperatures between 7°C and 11°C can be considered as appropriate for storage of eggs in ovarian fluid for up to 10 h. This information can have practical application to routine hatchery practice for acipenserids, as well as for certain research protocols.

Early Weaning Effects on Survival, Growth, and Histopathology of Larval Sterlet Acipenser ruthenus

“…; Linhart et al. ). Now Sterlet is third in terms of the frequency of production, coming after Siberian Sturgeon Acipenser baeri and Russian Sturgeon Acipenser gueldenstaedti , and it is grown in 15 different countries (Bronzi et al.…”

Comparative coelomic fluid composition of sterlet sturgeon Acipenser ruthenus Linnaeus, 1758, Siberian sturgeon Acipenser baerii Brandt, 1869, and Russian sturgeon Acipenser gueldenstaedtii Brandt & Ratzeburg, 1833

“…The results of the present study may be useful to develop some artificial media for washing and short‐term storage of sturgeon eggs, which would be useful for transportation to the hatchery of gametes after stripping to synchronize insemination (Linhart et al., ; Suquet et al., ). Several artificial media were developed based on the ionic composition of body fluids and successfully used to store unfertilized eggs of rainbow trout ( Oncorhynchus mykiss ), Persian sturgeon ( Acipenser persicus ), and brown trout ( Salmo trutta ; Goetz & Coffman, ; Niksirat, Sarvi, Mojazi Amiri, & Hatef, ; Niksirat, Sarvi, Mojazi Amiri, Karami et al., ; Sohrabnezhad et al., ).…”

“…Sturgeon (Chondrostean, Acipenseridae) as one of the ancient groups of fishes are widely known for their caviar but critically endangered by overexploitation and various anthropogenic conditions. Assisted propagation of several types of sturgeon is becoming increasingly important for recovery efforts as well as for commercial production of these endangered species (Linhart, Shelton, Tučková, Rodina, & Siddique, ; Siddique, Butts, Psenicka, & Linhart, ). Although CF biochemistry plays a very important role in preserving the egg fertilizing ability and influences sperm motility traits, few studies have explored the CF biochemistry of Acipenserids.…”

Effects of feeding rate on the growth performance of gynogenetic albino sterlet,Acipenser ruthenus(Linnaeus, 1758) larvae

“…Sterlet Acipenser ruthenus is one of the leading candidate species for sturgeon aquaculture (Linhart, Shelton, Tučková, Rodina, & Siddique, 2016). Sterlet is the third most widely cultured species among acipenserids, being farmed in 15 countries (Bronzi, Rosenthal, & Gessner, 2011).…”