Effects of temperature on ether lipid composition of Caldariella acidophila

Rosa, Mario De; Esposito, Enrico; Gambacorta, Agata; Nicolaus, Barbara; Bu’Lock, J. D.

doi:10.1016/0031-9422(80)85120-x

Cited by 200 publications

(145 citation statements)

References 12 publications

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“…Surprisingly, little is known about membrane adaptation mechanisms of archaea to temperature. Insertion of more pentacyclic rings can increase thermal tolerance in archaea with membrane-spanning glycerol diphytane glycerol tetra ether lipids (Derosa et al, 1980), but these compounds were not detected ( Supplementary Methods 1.3).…”

Section: Discussionmentioning

confidence: 99%

Metagenomic and lipid analyses reveal a diel cycle in a hypersaline microbial ecosystem

et al. 2015

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Marine microbial communities experience daily fluctuations in light and temperature that can have important ramifications for carbon and nutrient cycling. Elucidation of such short time scale community-wide dynamics is hindered by system complexity. Hypersaline aquatic environments have lower species richness than marine environments and can be well-defined spatially, hence they provide a model system for diel cycle analysis. We conducted a 3-day time series experiment in a well-defined pool in hypersaline Lake Tyrrell, Australia. Microbial communities were tracked by combining cultivation-independent lipidomic, metagenomic and microscopy methods. The ratio of total bacterial to archaeal core lipids in the planktonic community increased by up to 58% during daylight hours and decreased by up to 32% overnight. However, total organism abundances remained relatively consistent over 3 days. Metagenomic analysis of the planktonic community composition, resolved at the genome level, showed dominance by Haloquadratum species and six uncultured members of the Halobacteriaceae. The post 0.8 μm filtrate contained six different nanohaloarchaeal types, three of which have not been identified previously, and cryo-transmission electron microscopy imaging confirmed the presence of small cells. Notably, these nano-sized archaea showed a strong diel cycle, with a pronounced increase in relative abundance over the night periods. We detected no eukaryotic algae or other photosynthetic primary producers, suggesting that carbon resources may derive from patchily distributed microbial mats at the sediment-water interface or from surrounding land. Results show the operation of a strong community-level diel cycle, probably driven by interconnected temperature, light abundance, dissolved oxygen concentration and nutrient flux effects.

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Section: Discussionmentioning

confidence: 99%

Metagenomic and lipid analyses reveal a diel cycle in a hypersaline microbial ecosystem

et al. 2015

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“…In Bacillus spp., temperature adaptation is regulated by changing iso/anteiso fatty acid composition. 75) In some archaea, control of the property of hydrocarbon chains is carried out by regulation of number of cyclopentane rings (Sulfoloobus solfataricus), 76) and by regulation of the ratio of caldarchaeol/cyclic archaeol/archaeol (Methanocaldcoccus jannschii).…”

Section: Physiological Significance Of Archaeal Lipids-what Is a mentioning

confidence: 99%

Recent Advances in Structural Research on Ether Lipids from Archaea Including Comparative and Physiological Aspects

Koga

Morii

2005

Bioscience, Biotechnology, and Biochemistry

249

194

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“…The number of cyclopentane rings estimated by liquid SIMS is that estimated for the dominant species of the core lipid. Thus, we investigated the average number of cyclopentane rings in an isopranoid chain by GC analysis (7,39). First, TMS derivatives of biphytanyl alcohols in fraction P2 were analyzed by GC-MS. GC peaks with M ϩ values of 665.5 and 738.6 were assigned as the derivatives of biphytanyl alcohol with no cyclization, since both peaks were accompanied by the fragment ions at m/z values of 558.5 and 633.5, which are unique fragment ions of the noncyclized component (39).…”

Section: Liquid Sims Spectra Relative Masses Of the Deprotonated Maimentioning

confidence: 99%

Complete Polar Lipid Composition of Thermoplasma acidophilum HO-62 Determined by High-Performance Liquid Chromatography with Evaporative Light-Scattering Detection

Shimada¹,

Nemoto²,

Shida

et al. 2002

J Bacteriol

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Polar ether lipids of Thermoplasma acidophilum HO-62 were purified by high-performance liquid chromatography with an evaporative light-scattering detector. Structures of purified lipids were investigated by capillary gas chromatography, mass spectrometry, and nuclear magnetic resonance. Three types of ether lipids were found: phospholipids, glycolipids, and phosphoglycolipids. The two phospholipids had glycerophosphate as the phosphoester moiety. The seven glycolipids had different combinations of gulose, mannose, and glucose, which formed mono-or oligosaccharides. The eight phosphoglycolipids with two polar head groups contained glycerophosphate as the phosphoester moiety and gulose alone or gulose and mannose, which formed monoor oligosaccharides, as the sugar moiety. Although gulose is an unusual sugar in nature, several glyco-and phosphoglycolipids contained gulose as one of the sugar moieties in Thermoplasma acidophilum. All the ether lipids had isopranoid chains of C 40 or C 20 with zero to three cyclopentane rings. The structures of these lipids including four new glycolipids and three new phosphoglycolipids were determined, and a glycosylation process for biosynthesis of these glycolipids was suggested.Archaea have unique plasma membranes made of isopranoid glycerol ethers (10,14). The isopranoid chains of the archaeal membrane lipids consist of C 20 , C 25 , and C 40 hydrocarbons. The C 20 and/or C 25 chains are linked to glycerol to form a 2,3-di-O-alkyl-sn-glycerol diether (6, 15), and the C 40 (biphytanyl) chains are connected to two glycerols to form dibiphytanyl diglyceryl tetraether (6,14,19). Although the basic structures of the core lipids of archaea are common, many types of polar head groups have been reported (for reviews, see references 10 and 32). Investigation of the structures of the archaeal lipids not only is useful for rapid identification of archaea as chemical markers (10) but may provide evolutionary insights and the knowledge of how they can adapt to the extreme environments (9,13,16,17,20).Thermoplasma acidophilum is a facultative anaerobic, thermophilic, and acidophilic archaeon and grows optimally at pHs 1 to 2 and at 55 to 59°C. Tetraether-type glycolipids that have glucose and gulose (37) and lipopolysaccharide composed of mannose and glucose (31) have already been reported. Polar lipids are dominated by an MPL that occupies about half of the total lipids in Thermoplasma. The structures of MPL have been determined as ␤-L-gulopyranosyl-caldarchaetidyl-glycerol (35). Gulose is an unusual sugar component in nature. Some polar lipids have been resolved using thin-layer chromatography (19), and diglycosyl and triglycosyl variations of MPL-like lipids and phosphatidylglycerol diether lipid have been tentatively assigned based on the negativeion fast atom bombardment-mass spectrometry spectrum of the total lipid extract (35); however, the detailed properties of the polar lipids remain ambiguous.HPLC has been used to detect and separate ester lipids (8, 29). However, it is difficult t...

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Effects of temperature on ether lipid composition of Caldariella acidophila

Cited by 200 publications

References 12 publications

Metagenomic and lipid analyses reveal a diel cycle in a hypersaline microbial ecosystem

Metagenomic and lipid analyses reveal a diel cycle in a hypersaline microbial ecosystem

Recent Advances in Structural Research on Ether Lipids from Archaea Including Comparative and Physiological Aspects

Complete Polar Lipid Composition of Thermoplasma acidophilum HO-62 Determined by High-Performance Liquid Chromatography with Evaporative Light-Scattering Detection

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