Effects of temperature during soybean seed development on defense-related gene expression and fungal pathogen accumulation

Abstract

Soybean [Glycine max (L.) Merr] plants were exposed to three temperature regimens during seed development to investigate the effect of temperature on the expression of eight defense-related genes and the accumulation of two fungal pathogens in inoculated seeds. In seeds prior to inoculation, either a day/night warm (34/26 °C) or a cool temperature (22/18 °C) relative to normal (26/22 °C) resulted in altered patterns of gene expression including substantially lower expression of PR1, PR3 and PR10. After seed inoculation with Cercospora kikuchii, pathogen accumulation was lowest in seeds produced at 22/18 °C in which of all defense genes, MMP2 was uniquely most highly induced. For seeds inoculated with Diaporthe phaseolorum, pathogen accumulation was lowest in seeds produced at 34/26 °C in which of all defense genes, PR10 was uniquely most highly induced. Our detached seed assays clearly demonstrated that the temperature regimens we applied during seed development produced significant changes in seed defense-related gene expression both pre- and post inoculation and our findings support the hypothesis that global climate change may alter plant-pathogen interactions and thereby potentially crop productivity.

DL-β-Aminobutyric Acid-Induced Resistance in Soybean against Aphis glycines Matsumura (Hemiptera: Aphididae)

“…glycinea and was also activated in response to wounding and dehydration in soybean [39]. MMP2 was increased in soybean leaves and seeds in response to the fungal pathogens [79]. In our study, MMP2 was found to participate in pest defense such as SA, revealing its broad-spectrum resistance in biotic stress.…”

Isolation and identification of a mercury resistant strain

“…Impacts of temperature and pH on Hg(II) removal by the isolate. Various incubation temperatures (10, 20, 30, 40 and 50 °C) were applied to investigate the efficiencies of Hg(II) removal by the isolate [10]. The concentration of Hg(II) in the LB medium was kept 30 mg•dm 3 .…”

“…heavy metal biological absorption have shown that temperature and pH are important parameters affecting the process[10].…”

Impact des changements climatiques sur les interactions moléculaires entre le nématode à kyste du soya (Heterodera glycines) et son hôte principal, le soya (Glycine max)

“…De plus, chez la tomate, la hausse du CO2 atmosphérique aurait également un effet inhibiteur sur les taux de production des phytohormones (AJ et AS) qui lui confèrent une résistance à différents nématodes à galles (Cooper et al 2005;Sun et al 2010Sun et al , 2011. Pour le soya, les températures élevées ont un effet négatif sur plusieurs gènes de défense contre différents champignons pathogènes (Upchurch et Ramirez 2011).…”