Effects of temperature and salinity on oxygen consumption and ammonia excretion in different colour strains of the Manila clam,<i>Ruditapes philippinarum</i>

Nie, Hongtao; Chen, Peng; Huo, Zhongming; Chen, Yun; Hou, Xiaolin; Yang, Feng; Yan, Xiwu

doi:10.1111/are.13111

Cited by 49 publications

(25 citation statements)

References 34 publications

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“…Color is one of the most conspicuous phenotypic traits in R. philippinarum , and the diverse colors of molluscan shells are generally believed to be determined by the presence of biological pigments (Mann and Jackson, 2014, Lemer et al., 2015, Feng et al., 2018). Several studies have been conducted of the physiological, biochemical, and molecular responses to different types of biotic and abiotic stress in different shell color strains of the Manila clam (Zhang and Yan, 2010, Nie et al., 2017a, Nie et al., 2017b). In recent years, RNA sequencing (RNA-seq) has been used to identify the genes differentially expressed in the differently colored shells of several bivalves (Sun et al., 2015, Feng et al., 2015, Yue et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity

et al. 2019

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SummaryRuditapes philippinarum is an economically important bivalve with remarkable diversity in its shell coloration patterns. In this study, we sequenced the whole genome of the Manila clam and investigated the molecular basis of its adaptation to hypoxia, acidification, and parasite stress with transcriptome sequencing and an RNA sequence analysis of different tissues and developmental stages to clarify these major issues. A number of immune-related gene families are expanded in the R. philippinarum genome, such as TEP, C3, C1qDC, Hsp70, SABL, and lysozyme, which are potentially important for its stress resistance and adaptation to a coastal benthic life. The transcriptome analyses demonstrated the dynamic and orchestrated specific expression of numerous innate immune-related genes in response to experimental challenge with pathogens. These findings suggest that the expansion of immune- and stress-related genes may play vital roles in resistance to adverse environments and has a profound effect on the clam's adaptation to benthic life.

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Section: Introductionmentioning

confidence: 99%

Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity

et al. 2019

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“…Beyond critical temperature limits, metabolic depression or anaerobic energy production occurs, with stress protection mechanisms providing some limited plasticity 30 . In marine organisms, temperature has been shown to have profound effects on physiological functions such as oxygen consumption [32][33][34] , heart rates [35][36][37] , feeding 38,39 and activity 40 . Ectotherms, not able to control their body temperature, are most affected by changes in their thermal environmental 41,42 , which will likely be impacted by variability patterns.…”

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Warming and temperature variability determine the performance of two invertebrate predators

Lugo

Baumeister

Nour

et al. 2020

Sci Rep

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In a warming ocean, temperature variability imposes intensified peak stress, but offers periods of stress release. While field observations on organismic responses to heatwaves are emerging, experimental evidence is rare and almost lacking for shorter-scale environmental variability. for two major invertebrate predators, we simulated sinusoidal temperature variability (±3 °C) around todays' warm summer temperatures and around a future warming scenario (+4 °C) over two months, based on highresolution 15-year temperature data that allowed implementation of realistic seasonal temperature shifts peaking midpoint. Warming decreased sea stars' (Asterias rubens) energy uptake (Mytilus edulis consumption) and overall growth. Variability around the warming scenario imposed additional stress onto Asterias leading to an earlier collapse in feeding under sinusoidal fluctuations. Highpeak temperatures prevented feeding, which was not compensated during phases of stress release (low-temperature peaks). In contrast, increased temperatures increased feeding on Mytilus but not growth rates of the recent invader Hemigrapsus takanoi, irrespective of the scale at which temperature variability was imposed. This study highlights species-specific impacts of warming and identifies temperature variability at the scale of days to weeks/months as important driver of thermal responses. When species' thermal limits are exceeded, temperature variability represents an additional source of stress as seen from future warming scenarios.Global climate change is known to have profound impacts on marine ecosystems that range from distributional shifts and changes in species interactions to shifts in ecosystem function and diversity 1-4 . However, current knowledge on the impacts of climate change is based on experiments testing the effects of mean changes of stressors, rather than considering variability around means, or the role of climate extremes 5 .Environmental variability occurs at a variety of temporal (and spatial scales), from tidal to diurnal, over stochastic weekly to monthly patterns, and seasonal cycles, to large-scale inter-anneal variability. Irrespective of scale, this variability can impact organisms differently than changes in mean temperature (i.e. trends) 5,6 . High peaks of stressful temperatures might be lethal 7,8 , while excursions to below-stressful conditions can allow for stress relaxation 9 . Thus, in a warming ocean with already stressful mean temperature conditions 10 , environmental variability towards lower temperatures might create vital refuge from stress 9,11 . More theoretically, Jensen's inequality hypothesis postulates for non-linear functions (bell-shaped thermal performance curves, with an intermediate optimum), any variation around the mean should rise or lower the performance of an organism, when compared to constant environmental conditions 12 .Coastal waters are amongst the most valuable and productive ecosystems worldwide, providing a wide range of ecosystem services 1,13 . In the context of global climat...

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“…Compared with less-tolerant bivalves whose OCR decrease under moderate hypoxia [37], the Manila clam exhibited a progressive oxygen regulatory capacity, reflected in the relatively constant values of OCR, even at 1.0 mg L -1 DO. Combined with its strong tolerance to a wide range of water temperatures as well as salinities [38], the Manila clam can endure some of the most challenging shallow coastal environments. Slightly different from the values for OCR, AER was depressed in both the 0.5 and 1.0 mg L -1 DO treatment groups, providing evidence that, in Manila clam, protein catabolism is sensitive to reduced oxygen levels.…”

Section: Discussionmentioning

confidence: 99%

Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum)

Sun

Zhang

et al. 2019

PLoS ONE

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The Manila clam Ruditapes philippinarum has become a common and dominant macrobenthic species in coastal areas of the northwestern Pacific and temperate waters of Europe; it is also a major cultured shellfish, with annual worldwide production exceeding 3.3 million tonnes. This species faces greater risk of exposure to hypoxia as eutrophication worsens throughout its coastal habitats; however, its tolerance to hypoxia remains unclear, and the toxicological indicators including LC 50 and LT 50 have not yet been assessed. Previous studies on the effects of hypoxia on marine benthos have focused largely on functional responses, such as metabolism and gene expression, leaving potential structural damage to the mitochondria or the cells unknown. In this study we assessed the effects of hypoxia on Manila clam in terms of survival, behavior, metabolism and cellular damage, using a newly designed automated hypoxia simulation device that features exceptional accuracy and good stability. The clams exhibited strong tolerance to hypoxia as the 20-day LC 50 for dissolved oxygen (DO) was estimated to be 0.57 mg L -1 , and the LT 50 at 0.5 mg L -1 DO was 422 hours. Adaptations included fewer buried clams and a depressed metabolism, while the unexpected rise in the activities of key enzymes involved in glycolysis may indicate a diverse strategy of shellfish under hypoxia. Cellular damage was observed as collapse of the mitochondrial cristae and both cellular and mitochondrial vacuolization. This multi-level study complements and updates our knowledge of the effects of hypoxia on marine benthos, by improving our understanding of the potential for marine ecological transformation under hypoxic conditions and providing useful information for Manila clam farming.

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Effects of temperature and salinity on oxygen consumption and ammonia excretion in different colour strains of the Manila clam,Ruditapes philippinarum

Cited by 49 publications

References 34 publications

Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity

Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity

Warming and temperature variability determine the performance of two invertebrate predators

Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum)

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